FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l02_n02_DI244G4_P3CW_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l02_n02_DI244G4_P3CW_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3523643
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG192730.5469623341524666No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG133300.3783016610933628No Hit
AATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA132860.37705295343484No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT129820.3684255187032285No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA129410.36726195020324137No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC127570.3620400818130554No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA97950.27797935261886636No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT96210.2730412814237992No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAA96010.27247368703356156No Hit
GAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATA93190.26447060613121137No Hit
TCCTCATTCTGGGCAAAGAGGACAAGAGATATGGGCCAGCACTAAGCATC89300.25343089524108997No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT83680.23748149287541334No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCC81030.22996086720476508No Hit
GTAGAAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTT80240.22771886936332653No Hit
AGCGAAAGCAGGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAAG78720.22340515199752073No Hit
AGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGC76900.21824004304635855No Hit
GATGTAAGGCTTGCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATC75830.2152034130585874No Hit
ATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAAAACCACCGTG73820.20949908943669945No Hit
GGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATG72360.20535565038796497No Hit
TGATAACACAGTTCGAGTCTCTGAAACTCTACAGAGATTCGCTTGGAGAA70140.19905535265632757No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT69320.19672821565635337No Hit
AAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCCGAAT67590.19181852418079812No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT66320.18821429980278934No Hit
CCTCAGCAGTATGTCCTGGAAGAGAAGGCAATGGTGAAATTTCGCCAACA64170.18211266010773508No Hit
GACATAATGGATCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCT63620.18055177553458168No Hit
GGTCCATGCTCATACCCAAGCAGAAAGTGGCAGGCCCTCTTTGTATCAGA62030.17603940013219274No Hit
TCTCTGTAGAGTTTCAGAGACTCGAACTGTGTTATCATTCCATTCAAGTC60800.1725486946322315No Hit
CTACAGAGATTCGCTTGGAGAAGCAGTAATGAGAATGGGAGACCTCCACT60600.17198110024199387No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACATCCCCTTGC60360.17129998697370874No Hit
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT60090.17053373454688797No Hit
TCAATGAACTGAGCAACCTTGCGAAAGGAGAGAAGGCTAATGTGCTAATT57850.16417667737622682No Hit
GATACAAAGAGGGCCTGCCACTTTCTGCTTGGGTATGAGCATGGACCAGT57600.16346718438842983No Hit
GTTTTGGAGTGAGTGGAGGTCTCCCATTCTCATTACTGCTTCTCCAAGCG56250.159635922254326No Hit
GTTCTTATCTCTTGCTCCACTTCAAGCAATAGATGTAAGGCTTGCATAAA55810.15838721459580327No Hit
CTCTTGCTCCACTTCAAGCAATAGATGTAAGGCTTGCATAAATGTTATTT55420.15728040553483993No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA55130.15645739366899541No Hit
TCTTATTTCTTCAAACTTCTGACCTAATTGTTCCCGCCATTTCTCGTTTC53600.15211529658367776No Hit
GAATGGACCAGGCGATCATGGATAAGAACATCATACTGAAAGCGAACTTC53290.15123552527880946No Hit
GAATGAGGAATCCCCTCAGTCTTCTCCTGTCTTCCTGATGTGTACTTCTT52360.14859621136420462No Hit
GATAAGAACATCATACTGAAAGCGAACTTCAGTGTGATTTTTGACCGGCT52170.14805699669347888No Hit
GAGTGGAGGTCTCCCATTCTCATTACTGCTTCTCCAAGCGAATCTCTGTA51810.1470353267910512No Hit
CTTTGTATCAGAATGGACCAGGCGATCATGGATAAGAACATCATACTGAA51730.14680828903495616No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT50150.14232429335207908No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA50100.1421823947545197No Hit
AAACTATTCTCTGTTATCTTCAGTTTGTGTCTCACTTCTTCAATCAACCA50070.14209725559598405No Hit
CTCCAAAACAGAAACGAGAAATGGCGGGAACAATTAGGTCAGAAGTTTGA49580.1407066493399019No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA49160.13951470112040296No Hit
ACCTAACTGACATGACTCTTGAGGAAATGTCAAGGGACTGGTCCATGCTC48740.13832275290090398No Hit
CTGCTGAGGATGTCAAAAATGCAGTTGGAGTCCTCATCGGGGGACTTGAA48420.13741460187652382No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG47610.13511584459606152No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA46130.13091564610830325No Hit
GGAGAAGACTGAGGGGATTCCTCATTCTGGGCAAAGAGGACAAGAGATAT45710.12972369788880428No Hit
GGTCCATTCTGATACAAAGAGGGCCTGCCACTTTCTGCTTGGGTATGAGC45510.12915610349856668No Hit
GTTCTTATCCATGATCGCCTGGTCCATTCTGATACAAAGAGGGCCTGCCA45060.12787901612053207No Hit
GGTACAGAGGCCATGGTCATTTTAAGTGCCTCATCGGATTCTTCTTTCAG44950.1275668392059014No Hit
GTCTCGCACCCGCGAGATACTCACAAAAACCACCGTGGACCATATGGCCA44730.12694248537664005No Hit
TCTCATTACTGCTTCTCCAAGCGAATCTCTGTAGAGTTTCAGAGACTCGA44570.12648840986444995No Hit
ATGTAAGGCTTGCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATCT44290.1256937777181173No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG43910.12461534837666585No Hit
GTCTTCTCCTGTCTTCCTGATGTGTACTTCTTGATTATGGCCATATGGTC43700.12401937426691637No Hit
CTGTACCTGCGTCGCGTTACCTAACTGACATGACTCTTGAGGAAATGTCA43050.12217469249864416No Hit
ATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTC42970.12194765474254912No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT42690.12115302259621648No Hit
ATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTC42370.1202448715718363No Hit
ATCAAGAAGTACACATCAGGAAGACAGGAGAAGACTGAGGGGATTCCTCA42330.12013135269378879No Hit
AGTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATC42120.11953537858403931No Hit
GTTCATTGATGCTTAGTGCTGGCCCATATCTCTTGTCCTCTTTGCCCAGA42100.11947861914501554No Hit
CTTCTGACCTAATTGTTCCCGCCATTTCTCGTTTCTGTTTTGGAGTGAGT41920.1189677841938017No Hit
TCCTAGCTCCAGTGCTGGTCTGAAAAATGATCTTCTTGAAAATTTGCAGG41820.11868398699868289No Hit
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCTGCTGT41620.11811639260844528No Hit
GTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCCGCAAACGAGT41110.11666902691333941No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACAT40890.11604467308407804No Hit
GAACTAGGTGATGCCCCATTCCTTGATCGGCTTCGCCGAGATCAGAAATC40660.1153919395353048No Hit
GCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATCTTCAGTTTGTGT40320.11442702907190087No Hit
CTTAAAATGACCATGGCCTCTGTACCTGCGTCGCGTTACCTAACTGACAT40050.1136607766450801No Hit
ACCTAATTGTTCCCGCCATTTCTCGTTTCTGTTTTGGAGTGAGTGGAGGT39950.1133769794499613No Hit
GCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATTTCACCAT39880.11317832141337814No Hit
CATTACTGCTTCTCCAAGCGAATCTCTGTAGAGTTTCAGAGACTCGAACT39880.11317832141337814No Hit
CCCTAAGAGGAAGGGGCAGCACCCTCGGTCTGGACATCGAGACAGCCACA39540.11221341094997421No Hit
CCTCCACTCACTCCAAAACAGAAACGAGAAATGGCGGGAACAATTAGGTC39540.11221341094997421No Hit
CTTCCAGGACATACTGCTGAGGATGTCAAAAATGCAGTTGGAGTCCTCAT39500.1120998920719267No Hit
GTTATCTTCAGTTTGTGTCTCACTTCTTCAATCAACCATCTTATTTCTTC39390.111787715157296No Hit
GTGCTGGCCCATATCTCTTGTCCTCTTTGCCCAGAATGAGGAATCCCCTC38290.10866594601098921No Hit
CTGTTATCTTCAGTTTGTGTCTCACTTCTTCAATCAACCATCTTATTTCT38040.10795645302319218No Hit
CTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAAT37560.10659422648662195No Hit
GTCGTACTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA37520.10648070760857441No Hit
GCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGCT37490.1063955684500388No Hit
TTCTGATACAAAGAGGGCCTGCCACTTTCTGCTTGGGTATGAGCATGGAC37350.10599825237687246No Hit
GTCCATGCTCATACCCAAGCAGAAAGTGGCAGGCCCTCTTTGTATCAGAA37170.1054874174256586No Hit
GGTATGTGCAACCTGTGAACAGATTGCTGACTCCCAGCATCGGTCTCATA36960.10489144331590912No Hit
GTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGTATGATGTT36900.10472116499883787No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCCCCGTTCCC36040.10228050912081615No Hit
GCATGGACCAGTCCCTTGACATTTCCTCAAGAGTCATGTCAGTTAGGTAA35690.10128721893790035No Hit
GTATGATGTTCTTATCCATGATCGCCTGGTCCATTCTGATACAAAGAGGG35550.10088990286473404No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA10550.0131.346161
AGCGAAA33100.097.317611
GCAAAAG55150.095.7684253
AGCAAAA56350.093.986492
CCACGGG259.3938067E-486.98816145
ACTAATA3650.075.497038
ATCCGCC1450.074.98979145
TCTCGGG10850.074.82852145
ATCGCCC1000.072.490135145
CGTAGGG300.001937195472.49013145
ATTAGTA3850.071.607641
TACTAAT4000.068.888117
AGGTGTT2850.068.67486145
ATGTGGG1200.066.44928145
TAAACTA46700.064.471495
TTTTTAA46700.064.471491
GCAGGGT48400.063.5271879
AAGGGTG38050.062.7303475
TTTTAAA48000.062.5742382
GAAACAA53750.062.4940954
Produced by FastQC (version 0.11.5)