FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l02_n02_DI222G4_P2CW_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l02_n02_DI222G4_P2CW_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4119949
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG497741.2081217510216753No Hit
ATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA450651.0938242196687387No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA369090.8958606040997109No Hit
GAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATA241420.5859781273991499No Hit
AGCGAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAG226030.5486232960650727No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT219300.5322881424017627No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCC207140.5027732139402696No Hit
AAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCCGAAT180490.4380879472051717No Hit
ATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAAAACCACCGTG178230.43260244240887447No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT173000.4199081105130185No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTTGC163500.396849572652477No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG150520.3653443282914424No Hit
TCAATGAACTGAGCAACCTTGCGAAAGGAGAGAAGGCTAATGTGCTAATT150340.36490742967934797No Hit
GGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATG137200.33301383099645165No Hit
ATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACCCAGCACTTAGGAT129930.31536798149685835No Hit
GTTCATTGATGCTTAGTGCTGGCCCATATTTCCGTTATCCTCTTGTCTGC129370.3140087413703422No Hit
ATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTC128300.31141162184289173No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA123730.3003192515247155No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG123300.299275549284712No Hit
GTCTCGCACCCGCGAGATACTCACAAAAACCACCGTGGACCATATGGCCA116790.283474382813962No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA116280.2822365034130277No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT114120.2769937200678941No Hit
AGTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATC112910.27405679050881454No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGT112380.2727703668176475No Hit
GAGCGAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA109540.26587707760460144No Hit
GCTTAGTGCTGGCCCATATTTCCGTTATCCTCTTGTCTGCTGTAATTGGA108220.26267315444924194No Hit
GTGCTGGCCCATATTTCCGTTATCCTCTTGTCTGCTGTAATTGGATATTT99810.24226028040638367No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC98850.23993015447521318No Hit
ATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACC92180.22374063368260141No Hit
GCACTAAGCATCAATGAACTGAGCAACCTTGCGAAAGGAGAGAAGGCTAA91190.22133769131608183No Hit
ATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTC90390.21939591970677308No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT88500.21480848427978114No Hit
TCGCAAGGTTGCTCAGTTCATTGATGCTTAGTGCTGGCCCATATTTCCGT86810.2107064917551164No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA86360.20961424522488023No Hit
ATCCTCTTGTCTGCTGTAATTGGATATTTCATTGCCATCATCCATTTCAT86230.20929870733836756No Hit
GCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAGA86110.2090074415969712No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT85190.20677440424626617No Hit
GAAATATCCAATTACAGCAGACAAGAGGATAACGGAAATATGGGCCAGCA84120.20417728471881566No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA80050.1942985216564574No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA78750.19114314279133066No Hit
GGCCAGCACTAAGCATCAATGAACTGAGCAACCTTGCGAAAGGAGAGAAG77810.18886156115039288No Hit
ATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTTGCCC77260.18752659316899312No Hit
GGATATTTCATTGCCATCATCCATTTCATCCTAAGTGCTGGGTTCTTCTC76330.1852692836731717No Hit
CCATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACC76230.18502656222200808No Hit
AATTAGCACATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGA76080.1846624800452627No Hit
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT75160.18242944269455763No Hit
CATCCATTTCATCCTAAGTGCTGGGTTCTTCTCCTGTCTTCCTGATGTGT75130.18235662625920854No Hit
GTACACATCAGGAAGACAGGAGAAGAACCCAGCACTTAGGATGAAATGGA73910.1793954245550127No Hit
GTGCTGGGTTCTTCTCCTGTCTTCCTGATGTGTACTTCTTGATTATGGCC72760.17660412786663138No Hit
GTAGAAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTT72100.17500216628895163No Hit
ACCCAGCACTTAGGATGAAATGGATGATGGCAATGAAATATCCAATTACA71760.1741769133549954No Hit
AGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAG71620.17383710332336638No Hit
CTTCTTGATTATGGCCATATGGTCCACGGTGGTTTTTGTGAGTATCTCGC70500.17111862307033412No Hit
GATGTAAGGCTTGCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATC69480.16864286426846548No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA68100.16529330824240784No Hit
CAATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCG67290.16332726448798274No Hit
GCCATATGGTCCACGGTGGTTTTTGTGAGTATCTCGCGGGTGCGAGACTG67250.1632301759075173No Hit
CATCAGGAAGACAGGAGAAGAACCCAGCACTTAGGATGAAATGGATGATG66510.1614340371689067No Hit
TGATAACACAGTTCGAGTCTCTGAAACTCTACAGAGATTCGCTTGGAGAA64770.15721068391866014No Hit
AGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGC63040.15301160281352996No Hit
CATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGATGCTTAGT60260.14626394647118204No Hit
CTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGG59670.14483188990931684No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC59430.1442493584265242No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT58980.14315711189628802No Hit
GAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAA58910.1429872068804735No Hit
CCTCAGCAGTATGTCCTGGAAGAGAAGGCAATGGTGAAATTTCGCCAACA58790.14269594113907721No Hit
GTGCTAATTGGGCAAGGAGACGTGGTGTTGGTAATGAAACGGAAACGGGA58620.1422833146720991No Hit
GGTCCATGCTCATACCCAAGCAGAAAGTGGCAGGCCCTCTTTGTATCAGA57560.139710467289765No Hit
AAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTT57120.13864249290464517No Hit
GTAATTGGATATTTCATTGCCATCATCCATTTCATCCTAAGTGCTGGGTT56910.13813277785720163No Hit
CACCACGTCTCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTCGCAA56840.1379628728413871No Hit
GGATGAAATGGATGATGGCAATGAAATATCCAATTACAGCAGACAAGAGG55900.1356812912004493No Hit
TCCAATTACAGCAGACAAGAGGATAACGGAAATATGGGCCAGCACTAAGC54770.13293853880230072No Hit
TAATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACCCAGCACTTAGG54560.13242882375485715No Hit
GATGTGTACTTCTTGATTATGGCCATATGGTCCACGGTGGTTTTTGTGAG54400.1320404694329954No Hit
GTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAA54120.13136084936973735No Hit
TTCCTGATGTGTACTTCTTGATTATGGCCATATGGTCCACGGTGGTTTTT54050.13119094435392284No Hit
TTTCATTGCCATCATCCATTTCATCCTAAGTGCTGGGTTCTTCTCCTGTC53660.13024433069438482No Hit
ATTTCATCCTAAGTGCTGGGTTCTTCTCCTGTCTTCCTGATGTGTACTTC53490.1298317042274067No Hit
TCCTAGCTCCAGTGCTGGTCTGAAAAATGATCTTCTTGAAAATTTGCAGG53360.12951616634089402No Hit
TCTCTGTAGAGTTTCAGAGACTCGAACTGTGTTATCATTCCATTCAAGTC51470.1249287309139021No Hit
CCTCTTGTCTGCTGTAATTGGATATTTCATTGCCATCATCCATTTCATCC51170.12420056656041131No Hit
ACCAACACCACGTCTCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTT51150.12415202227017859No Hit
CTACAGAGATTCGCTTGGAGAAGCAGTAATGAGAATGGGAGACCTCCACT50780.12325395290087328No Hit
ATATTTCCGTTATCCTCTTGTCTGCTGTAATTGGATATTTCATTGCCATC50410.122355883531568No Hit
CTAGCATACTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGGCC50340.12218597851575347No Hit
CTCTTGCTCCACTTCAAGCAATAGATGTAAGGCTTGCATAAATGTTATTT50240.12194325706458987No Hit
GACATAATGGATCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCT50190.12182189633900808No Hit
GAGAAGAACCCAGCACTTAGGATGAAATGGATGATGGCAATGAAATATCC50170.12177335204877535No Hit
TTACCAACACCACGTCTCCTTGCCCAATTAGCACATTAGCCTTCTCTCCT49860.12102091555016821No Hit
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCTGCTGT49640.12048692835760831No Hit
GATACAAAGAGGGCCTGCCACTTTCTGCTTGGGTATGAGCATGGACCAGT49450.12002575760039746No Hit
AAATGGATGATGGCAATGAAATATCCAATTACAGCAGACAAGAGGATAAC48920.11873933390923043No Hit
GAATGGACCAGGCGATCATGGATAAGAACATCATACTGAAAGCGAACTTC48850.11856942889341592No Hit
AAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAAAACCA48650.11808398599108873No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCCCCGTTCCC48450.11759854308876154No Hit
ATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACCCA48260.1171373723315507No Hit
GTTCTTATCTCTTGCTCCACTTCAAGCAATAGATGTAAGGCTTGCATAAA48190.11696746731573619No Hit
TCGCAGTCTCGCACCCGCGAGATACTCACAAAAACCACCGTGGACCATAT48120.11679756229992168No Hit
GAAGTACACATCAGGAAGACAGGAGAAGAACCCAGCACTTAGGATGAAAT47450.1151713285771256No Hit
GAGTGGAGGTCTCCCATTCTCATTACTGCTTCTCCAAGCGAATCTCTGTA47440.11514705643200923No Hit
GTTTTGGAGTGAGTGGAGGTCTCCCATTCTCATTACTGCTTCTCCAAGCG46880.1137878163054931No Hit
CTTTGTATCAGAATGGACCAGGCGATCATGGATAAGAACATCATACTGAA46250.11225867116316246No Hit
TCTTATTTCTTCAAACTTCTGACCTAATTGTTCCCGCCATTTCTCGTTTC45840.11126351321339172No Hit
GGTATGTGCAACCTGTGAACAGATTGCTGACTCCCAGCATCGGTCTCATA45230.10978291236129378No Hit
GATAAGAACATCATACTGAAAGCGAACTTCAGTGTGATTTTTGACCGGCT44930.10905474800780302No Hit
GAAATGGATGATGGCAATGAAATATCCAATTACAGCAGACAAGAGGATAA44690.10847221652501038No Hit
AAACTATTCTCTGTTATCTTCAGTTTGTGTCTCACTTCTTCAATCAACCA44500.10801104576779956No Hit
CTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACC44460.10791395718733413No Hit
GAGCAACCTTGCGAAAGGAGAGAAGGCTAATGTGCTAATTGGGCAAGGAG44450.10788968504221776No Hit
GACAAGAGGATAACGGAAATATGGGCCAGCACTAAGCATCAATGAACTGA44360.10767123573617052No Hit
GGAGAAGAACCCAGCACTTAGGATGAAATGGATGATGGCAATGAAATATC44310.10754987501058871No Hit
CTGCTGAGGATGTCAAAAATGCAGTTGGAGTCCTCATCGGGGGACTTGAA44220.10733142570454147No Hit
CTCCAAAACAGAAACGAGAAATGGCGGGAACAATTAGGTCAGAAGTTTGA44170.1072100649789597No Hit
TCTTGATTATGGCCATATGGTCCACGGTGGTTTTTGTGAGTATCTCGCGG43190.10483139475755646No Hit
ATTAGCACATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGAT43080.10456440116127651No Hit
GTCCACGGTGGTTTTTGTGAGTATCTCGCGGGTGCGAGACTGCGACATTA42620.10344788248592399No Hit
TACCAACACCACGTCTCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTT42390.10288962314824772No Hit
ACCTTGCGAAAGGAGAGAAGGCTAATGTGCTAATTGGGCAAGGAGACGTG42140.10228281952033873No Hit
CTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGGCCATCAATTA41910.10172456018266245No Hit
CAAGAGGATAACGGAAATATGGGCCAGCACTAAGCATCAATGAACTGAGC41710.10123911728033526No Hit
GATTATGGCCATATGGTCCACGGTGGTTTTTGTGAGTATCTCGCGGGTGC41610.10099639582917168No Hit
GGTACAGAGGCCATGGTCATTTTAAGTGCCTCATCGGATTCTTCTTTCAG41610.10099639582917168No Hit
ACCTAACTGACATGACTCTTGAGGAAATGTCAAGGGACTGGTCCATGCTC41500.1007294022328917No Hit
ATGTAAGGCTTGCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATCT41320.10029250362079724No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA19150.0135.628361
TTAGGGG256.471364E-6115.985306145
GTAATTT2400.0114.777115145
AGCAAAA92500.0109.412952
GCAAAAG99650.0101.562453
AGCGAAA52300.089.7509161
CAAAAGC127100.079.913244
TGACGCC6050.076.684494145
ACTAATA6200.076.025198
GACGCTC1550.074.82922145
GCCGACC6750.073.02778145
AGTAGAA41150.072.109041
GCAGGGT44800.071.8688669
GCAGGGG4850.071.7687459
TATTAGT3550.071.527981
CGAAAGC66100.070.903443
TACTAAT6650.070.880637
AGCAGGG50300.070.786328
AAGCAGG183000.069.821747
TACTCCC17700.067.57618145
Produced by FastQC (version 0.11.5)