FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l02_n01_WT_PR8_M2_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l02_n01_WT_PR8_M2_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3418441
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT215120.6292927097469284No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC188410.5511576768474284No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT167520.4900479487579279No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA149840.43832846610487064No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT147270.4308104191355065No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA130430.38154819697049036No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA110650.32368556309733004No Hit
GTTAAGGACAGAAGCCCTTATAGGGCCTTAATGAGCTGCCCTGTCGGTGA88850.25991380281245163No Hit
ATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA83230.2434735600234142No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG81710.23902708866410158No Hit
ATATACAGCAAAGACAATAGCATAAGAATTGGTTCCAAAGGAGACGTTTT79490.23253290023142129No Hit
GTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCGCTATACCCTG78470.22954908392451412No Hit
GTCCTTAACAGTCCCATTTGAATGCTTGTCATTCAGTAAGGCACCTTGGG74870.21901796754719474No Hit
GAAGCAGTTTTCTTGATTCAGCTGAAAATCCTTCTAGTTGTGGAGATGCA73100.2138401686616794No Hit
GTATATAGCCCACCCACGGATGGGACAAAGAGATGAATTGCCGGTTAATA71710.2097739876159922No Hit
GTTCATTGATGCTTAGTGCTGGCCCATATCTCTTGTCTTCTTTGCCCAGA67100.19628830803281377No Hit
TTTTTGAACAGACTACTTGTCAATGCTGAATGGCAACTCAGCACCGTCTG64610.18900428587183454No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTT63520.18581569785759064No Hit
GGTATGGTAATGGTGTTTGGATAGGAAGGACCAAAAGTCACAGTTCCAGA58510.17115989423248784No Hit
TAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAA58310.17057483221152567No Hit
GTATTAAAATACAACGGCATAATAACTGAAACCATAAAAAGTTGGAGGAA54430.15922462900485923No Hit
GCAATGAACTGAGCAACCTTGCGAAAGGAGAGAAGGCTAATGTGCTAATT53060.15521695416126827No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG52020.15217463165226489No Hit
GCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTC51140.14960035876003125No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA51060.1493663339516464No Hit
GTTCATTGCTGGCCCATATCTCTTGTCTTCTTTGCCCAGAATGAGGAATC48320.14135098426446444No Hit
CAATATGGATTAGCCATTCAATTCAAACTGGAAGTCAAAACCATACTGGA47930.14021011332358815No Hit
GCTGTATATAGCCCACCCACGGATGGGACAAAGAGATGAATTGCCGGTTA47760.1397128106057703No Hit
TACCATACCTATATGAAAATCCCTTTACTCCGTTTGCTCCATCAACATAC47610.13927401409004866No Hit
GCCTCATACAGTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCG46300.13544185785274632No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTTGC45560.13327712837518624No Hit
GGATTAGCCATTCAATTCAAACTGGAAGTCAAAACCATACTGGAATATGC44750.1309076271902894No Hit
GAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATA44130.1290939349253066No Hit
AGCGAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAG44080.12894766942006605No Hit
GACTACTTGTCAATGCTGAATGGCAACTCAGCACCGTCTGGCCAAGACCA44050.12885991011692172No Hit
ATATAGCCCACCCACGGATGGGACAAAGAGATGAATTGCCGGTTAATATC43850.12827484809595951No Hit
GCACTAAGCATCAATGAACTGAGCAACCTTGCGAAAGGAGAGAAGGCTAA43310.12669518063936164No Hit
GTGTAAATGGTTCATGTTTTACTATAATGACTGATGGCCCGAGTGATGGG43130.12616862482049565No Hit
CCCTTATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATACA42870.1254080441932448No Hit
GCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCATTG41890.12254124029053011No Hit
ATATGAAGCAATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTA39350.11511095262431031No Hit
ACTCAGCACCGTCTGGCCAAGACCAATCTACAGTATCACTATTCACGCCA38970.11399933478448217No Hit
GCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAGA38950.11394082858238595No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT38560.11279995764150968No Hit
GGATACATCTGCAGTGGGGTTTTCGGTGACAACCCGCGTCCCGAAGATGG38170.11165908670063342No Hit
GGGTAAAGGACACAACTTCAGTGATATTAACCGGCAATTCATCTCTTTGT37860.11075224056814202No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGTGAACTCTTCTTTCTTT36980.1081779676759084No Hit
ACATACACTGGACCACAGCTGCCTGTTCCATCTTCGGGACGCGGGTTGTC36470.10668605952245483No Hit
ATCAAATAGGATACATCTGCAGTGGGGTTTTCGGTGACAACCCGCGTCCC35930.10510639206585692No Hit
GGGCTAGACTGTATGAGGCCGTGCTTCTGGGTTGAATTAATCAGGGGACG35840.10484311415642393No Hit
GAGCGAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT35480.10379000251869201No Hit
CCATTTACACAGGCACATTCAGACTCTTGTGTCCTCAATATTTTCTTCCT35190.10294166258829683No Hit
CTTAGGGACAACCTTGAACCTGGGACCTTTGATCTTGGGGGGCTATATGA35060.10256137227467141No Hit
TTACCATACCTATATGAAAATCCCTTTACTCCGTTTGCTCCATCAACATA34710.10153751373798757No Hit
GTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAGCAAAACCCAGGGATCA34650.10136199513169893No Hit
GCCTAATATTGCAAATAGGGAATATAATCTCAATATGGATTAGCCATTCA34530.10101095791912162No Hit
GGAATATGCAACCAAAACATCATTACCTATAAAAATAGCACCTGGGTAAA34450.10077693311073674No Hit
CCCATGGCCGGTTCGAACCATGCCAGTTGTCTCTGCACACACACATCACT34440.10074768000968863No Hit
GTCTGGAACTGTGACTTTTGGTCCTTCCTATCCAAACACCATTACCATAC34370.10054290830235185No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGGT100.0070885634145.020814
GAGCGAA14700.0137.649981
AGCAGGC31100.0100.937288
GCAAAAG125500.098.9723743
CAAAAGC139400.089.1035464
ACCGGAG259.383351E-487.01253
GTATAAT353.436021E-582.869035
GCAGGGG34250.082.128769
AAGCAGG176550.081.839647
GCAGGGT14700.078.909049
AAAAGCA168850.073.6484155
GAGCAAA170700.073.5024641
AGCAGGG56050.070.8808758
AGCGAAA28550.070.859562
TATTAGT2100.069.071671
AAAGCAG210100.068.816586
GTAGTTG851.6916601E-1068.259071
GCAGGCA45800.068.22389
GCAGGTC38950.067.937499
TAAACTA20150.066.572835
Produced by FastQC (version 0.11.5)