FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l02_n01_WT_PR8_F1_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l02_n01_WT_PR8_F1_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2758245
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT209930.7610999022929436No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTT132360.4798703523436098No Hit
CCATAGCCTCCACCATACTGGATATTCCGACTGGTCTTCTGTATGAACTG92290.3345968179041383No Hit
CCCTTATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATACA91620.33216773709369546No Hit
GGACATGGTTTCCAGAGCCCGAATTGATGCACGGATTGATTTCGAATCTG77900.2824259628858205No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA75850.2749937007046147No Hit
CCTTACAGCCATGGGACAGGAACAGGATACACCATGGATACTGTCAACAG74120.2687215965224264No Hit
ATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATACACCATG69410.2516455209743877No Hit
ATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA68300.24762122291529576No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC67750.24562720135448446No Hit
TTCTTTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGGGCTCT66030.23939135210976542No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG65660.23804991942340145No Hit
GGATACACCATGGATACTGTCAACAGGACACATCAGTACTCAGAAAAGGG63470.2301100881176255No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT62890.2280073017444063No Hit
GGACACATCAGTACTCAGAAAAGGGAAGATGGACAACAAACACCGAAACT62540.22673837893298093No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA60460.21919735193936724No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA60350.218798547627205No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGTGAACTCTTCTTTCTTT57720.20926349907277997No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT55560.20143243257941193No Hit
GTTAAGGACAGAAGCCCTTATAGGGCCTTAATGAGCTGCCCTGTCGGTGA54710.1983507628945217No Hit
GCTTTTCAAAAATACCAGGATGGGATTCCTCAAGGAAAGCCATTGCTTCC50890.1845013767812504No Hit
ATTTTGTGCGACAATGCTTCAATCCGATGATTGTCGAGCTTGCGGAAAAA46850.16985438204365458No Hit
ATATACAGCAAAGACAATAGCATAAGAATTGGTTCCAAAGGAGACGTTTT45430.16470618092301445No Hit
GTCCTTAACAGTCCCATTTGAATGCTTGTCATTCAGTAAGGCACCTTGGG44710.1620958254252251No Hit
CTTAGGGACAACCTTGAACCTGGGACCTTTGATCTTGGGGGGCTATATGA44680.1619870606128172No Hit
GAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT44530.16144323655077775No Hit
CTTCTTTCTTTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGG44120.1599567841145366No Hit
GTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCGCTATACCCTG43840.15894164586539627No Hit
GGACAAGCTAAATTCACTATTTTTGCCGTCTGAGCTCTTCAATGGTGGAA42100.15263328674573867No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTTTGCCG41670.15107432443455893No Hit
GTATATAGCCCACCCACGGATGGGACAAAGAGATGAATTGCCGGTTAATA41420.15016795099782654No Hit
GCTAAATTCACTATTTTTGCCGTCTGAGCTCTTCAATGGTGGAACAGATC40530.14694126156305912No Hit
CATCAGTACTCAGAAAAGGGAAGATGGACAACAAACACCGAAACTGGAGC39340.14262692400421284No Hit
GATCTTCATGATCTCAGTGAACTCTTCTTTCTTTATCCTTCCAGATTCGA37800.13704366363394116No Hit
TCTTAAAAGTGCCAGCACAAAATGCTATAAGCACAACTTTCCCTTATACT36420.13204048226317822No Hit
GTATTAAAATACAACGGCATAATAACTGAAACCATAAAAAGTTGGAGGAA36140.13102534401403793No Hit
GAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATA36050.13069904957681425No Hit
GCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTTTC35070.12714606570482317No Hit
GGTATGGTAATGGTGTTTGGATAGGAAGGACCAAAAGTCACAGTTCCAGA32760.11877117514941565No Hit
CAATATGGATTAGCCATTCAATTCAAACTGGAAGTCAAAACCATACTGGA32600.11819109614990692No Hit
ATCCAGTATGGTGGAGGCTATGGTTTCCAGAGCCCGAATTGATGCACGGA32500.11782854677521395No Hit
TACCATACCTATATGAAAATCCCTTTACTCCGTTTGCTCCATCAACATAC32010.11605205483921843No Hit
GGATTAGCCATTCAATTCAAACTGGAAGTCAAAACCATACTGGAATATGC31700.11492815177767023No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG31140.11289787527938962No Hit
TTTTTGAACAGACTACTTGTCAATGCTGAATGGCAACTCAGCACCGTCTG30510.11061381421882392No Hit
CTTCCAATACACAATCTGTTTGGGCATAACCACTTGGTTCATTGTCTTCT30460.11043253953147744No Hit
TCTTTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGGGCTCTG30440.11036002965653885No Hit
CCATTTGAATGGATGTCAATCCGACCTTACTTTTCTTAAAAGTGCCAGCA29820.10811222353344246No Hit
AGCGAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAG29620.10738712478405653No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT29080.1054293581607145No Hit
ATACTGGATATTCCGACTGGTCTTCTGTATGAACTGCTTTTCAAAAATAC28930.10488553409867506No Hit
TAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAA28780.10434171003663562No Hit
GCTGTATATAGCCCACCCACGGATGGGACAAAGAGATGAATTGCCGGTTA28620.10376163103712688No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTTT28440.10310904216267952No Hit
GTGTAAATGGTTCATGTTTTACTATAATGACTGATGGCCCGAGTGATGGG28150.10205764897606992No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA15750.0128.013061
GCAAAAG89400.098.4656143
AGCAGGC46300.095.9851768
AGTAGAA34350.086.143691
CAAAAGC107450.081.992354
GAGCAAA110000.081.0965651
AAGCAGG140300.077.4067467
GCAGGCA54000.077.196719
GTCACGA406.6537104E-572.525391
AGCGAAA28450.070.8542562
AGCAAAA127850.069.646782
GCAGGGG26700.068.153349
GTAGAAA47350.062.7866482
GCAGGGT11750.060.4662329
AGCAGGG55500.059.304498
CGCTCCC1600.058.9151276
AAAGCAG185900.058.5470126
AGGGTGT8800.058.5031366
CTATTCG17500.058.41259
AAGGGTG8700.058.3421255
Produced by FastQC (version 0.11.5)