FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l02_n01_WT_PR8_F1_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l02_n01_WT_PR8_F1_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2619586
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT213240.8140217576365121No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT99880.3812816223632284No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA96610.36879873384572986No Hit
CCATAGCCTCCACCATACTGGATATTCCGACTGGTCTTCTGTATGAACTG81360.3105834280684047No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTT79720.30432289682415464No Hit
CCCTTATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATACA66790.25496395231918323No Hit
GTTAAGGACAGAAGCCCTTATAGGGCCTTAATGAGCTGCCCTGTCGGTGA66410.2535133414211253No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT65650.25061211962500946No Hit
GGACATGGTTTCCAGAGCCCGAATTGATGCACGGATTGATTTCGAATCTG62090.23702218594846666No Hit
ATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA60730.2318305258922593No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA57740.22041650856280343No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA56340.21507215262258997No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC56300.2149194567385839No Hit
ATATACAGCAAAGACAATAGCATAAGAATTGGTTCCAAAGGAGACGTTTT56050.21396510746354577No Hit
GTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCGCTATACCCTG55220.2107966678704192No Hit
ATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATACACCATG54840.2093460569723613No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG52710.20121500114903654No Hit
GGATACACCATGGATACTGTCAACAGGACACATCAGTACTCAGAAAAGGG52600.20079508746801977No Hit
GTCCTTAACAGTCCCATTTGAATGCTTGTCATTCAGTAAGGCACCTTGGG51850.19793203964290543No Hit
GCTTTTCAAAAATACCAGGATGGGATTCCTCAAGGAAAGCCATTGCTTCC51230.19556525344081088No Hit
CCTTACAGCCATGGGACAGGAACAGGATACACCATGGATACTGTCAACAG49580.1892665482255593No Hit
ATTTTGTGCGACAATGCTTCAATCCGATGATTGTCGAGCTTGCGGAAAAA47440.18109731843123303No Hit
GTATATAGCCCACCCACGGATGGGACAAAGAGATGAATTGCCGGTTAATA44980.17170652156485797No Hit
GGACACATCAGTACTCAGAAAAGGGAAGATGGACAACAAACACCGAAACT44860.17124843391283967No Hit
GAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT44060.1681945162327177No Hit
TTCTTTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGGGCTCT40360.15407014696215357No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG40340.1539937990201505No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGTGAACTCTTCTTTCTTT39590.15113075119503616No Hit
GTATTAAAATACAACGGCATAATAACTGAAACCATAAAAAGTTGGAGGAA38170.14571004731281967No Hit
CTTAGGGACAACCTTGAACCTGGGACCTTTGATCTTGGGGGGCTATATGA37200.14200717212567177No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAAACCTACT35200.13437237792536683No Hit
GATCTTCATGATCTCAGTGAACTCTTCTTTCTTTATCCTTCCAGATTCGA35030.13372342041834093No Hit
GGTATGGTAATGGTGTTTGGATAGGAAGGACCAAAAGTCACAGTTCCAGA34370.13120393833224028No Hit
GCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTTTC33530.1279973247681122No Hit
CAATATGGATTAGCCATTCAATTCAAACTGGAAGTCAAAACCATACTGGA33350.12731019329008478No Hit
TACCATACCTATATGAAAATCCCTTTACTCCGTTTGCTCCATCAACATAC33080.1262794960730436No Hit
TTTTTGAACAGACTACTTGTCAATGCTGAATGGCAACTCAGCACCGTCTG32300.12330192633492468No Hit
GCCTCATACAGTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCG31870.12166044558185912No Hit
GAGCAAAAGCAGGGAGACAGGGAGGGAATCTGGGACTGAGAGGGGGAGGG31610.12066792233581948No Hit
GGACAAGCTAAATTCACTATTTTTGCCGTCTGAGCTCTTCAATGGTGGAA31450.1200571387997951No Hit
GTGTAAATGGTTCATGTTTTACTATAATGACTGATGGCCCGAGTGATGGG30050.11471278285958163No Hit
GAAGCAGTTTTCTTGATTCAGCTGAAAATCCTTCTAGTTGTGGAGATGCA29610.11303312813551454No Hit
GCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCATTG29550.11280408430950539No Hit
CATCAGTACTCAGAAAAGGGAAGATGGACAACAAACACCGAAACTGGAGC29240.11162069120845813No Hit
GACTACTTGTCAATGCTGAATGGCAACTCAGCACCGTCTGGCCAAGACCA29180.11139164738244899No Hit
GGATTAGCCATTCAATTCAAACTGGAAGTCAAAACCATACTGGAATATGC28970.11058999399141697No Hit
TAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAA28300.1080323379343148No Hit
GCTGTATATAGCCCACCCACGGATGGGACAAAGAGATGAATTGCCGGTTA28250.10784146807930718No Hit
AGCGAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAG28180.10757425028229652No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA27620.10543650790621113No Hit
CTTCTTTCTTTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGG27500.10497842025419284No Hit
TTACCATACCTATATGAAAATCCCTTTACTCCGTTTGCTCCATCAACATA27100.10345146141413186No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTTTGCCG26850.10249711213909374No Hit
GAGCGAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA26800.10230624228408612No Hit
GATCTATGATCTCAGTGAACTCTTCTTTCTTTATCCTTCCAGATTCGAAA26560.10139006698004951No Hit
CTTCCAATACACAATCTGTTTGGGCATAACCACTTGGTTCATTGTCTTCT26480.10108467521203733No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC100.0070896605145.012663
GAGCGAA19050.0133.642821
GCAAAAG103400.0101.186313
AGCAGGC47050.095.996338
GAGCAAA124200.084.855011
CAAAAGC125650.083.2683264
AAGCAGG158850.081.785647
AGCAAAA134200.078.4494862
AGCGAAA32200.078.360882
GCAGGCA55800.075.616239
AGTAGAA29150.073.901191
GCAGGGG34750.072.602359
AAAGCAG198800.065.5401846
GCAGGGT14800.065.150279
AGCAGGG66950.064.322378
AGCAGGT62800.063.7243848
AAAAGCA167950.062.512165
GCCGAAT350.00356591362.1482933
GCAGGTC29050.062.141179
CGAAAGC41850.060.8117684
Produced by FastQC (version 0.11.5)