FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l02_n01_WT_PBS_M1_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l02_n01_WT_PBS_M1_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2206854
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGAGACAGGGAGGGAATCTGGGACTGAGAGGGGGAGGG172360.7810213090671154No Hit
GAGTAGGGGCTGGTGGGAGAAGTGTGTGATGTGGACCATGGTAAAAGCTA79260.359153799934205No Hit
AAACAAGGTGGAGTAGGGGCTGGTGGGAGAAGTGTGTGATGTGGACCATG61860.28030852969883824No Hit
GGTGAGTGCAAAGGGTTGCAACTGAGCTGCCTTCTGTCATTTATCTAGCT54660.24768290063592788No Hit
GAGCAAAAGCAGGCCCAAGCAGCCAAAGAGAAGAAGAAGAGGAGGAGGAG51760.23454202226336676No Hit
GAGATAAGGAGGGAGGATGGGAAAGAAAGGGAGGGGAGGAGAGGTCAAGG51560.23363575478939705No Hit
GCAAAAGCAGGGAGACAGGGAGGGAATCTGGGACTGAGAGGGGGAGGGGA48420.21940735544807222No Hit
GAGCGAAAGCAGGGAGACAGGGAGGGAATCTGGGACTGAGAGGGGGAGGG44360.2010101257264867No Hit
AGCGAAAGCAGGGAGACAGGGAGGGAATCTGGGACTGAGAGGGGGAGGGG40440.1832472832366799No Hit
GTAATGGGCCCTGCTCCTTTGTGAGACAAAGGTGAGTGCAAAGGGTTGCA40100.18170662853093136No Hit
GGTGGGAGAAGTGTGTGATGTGGACCATGGTAAAAGCTACAGTAATGGGC39510.17903313948272065No Hit
AGTAGAAACAAGGTGGAGTAGGGGCTGGTGGGAGAAGTGTGTGATGTGGA38380.17391272825479165No Hit
AGCAAAAGCAGGGAGACAGGGAGGGAATCTGGGACTGAGAGGGGGAGGGG37330.16915482401645057No Hit
ATCTGGGACTGAGAGGGGGAGGGGAATGTTTGGAGGCAGAGAGAGAGAGG35870.1625390714564715No Hit
GAGCAAAAGCAGGGGTACCCTTGTAAGAAATTTTCACCACAGAAGGAGCC32990.14948881983130735No Hit
GAGCAAAAGCAGGCAAAAGAGTCAGAGACAGTCCCACTCCCACTGTTAGG32540.14744971801487547No Hit
GTAGAAACAAGGTGGAGTAGGGGCTGGTGGGAGAAGTGTGTGATGTGGAC31590.14314494751351925No Hit
GTGGAGTAGGGGCTGGTGGGAGAAGTGTGTGATGTGGACCATGGTAAAAG31540.14291838064502682No Hit
AGGTGGAGTAGGGGCTGGTGGGAGAAGTGTGTGATGTGGACCATGGTAAA31100.14092459220229342No Hit
GGGTTGCAACTGAGCTGCCTTCTGTCATTTATCTAGCTTTGCCTTCCCTC29570.13399164602642494No Hit
GAGACAGGGAGGGAATCTGGGACTGAGAGGGGGAGGGGAATGTTTGGAGG26600.12053357403797442No Hit
CAGGGAGACAGGGAGGGAATCTGGGACTGAGAGGGGGAGGGGAATGTTTG26340.11935542632181377No Hit
GAGCAAAAGCAGGAGGGATCAAGTAAGAGGGAGATGGAGGGAGAAATTAT25060.11355531448840749No Hit
AAAGCAGGGAGACAGGGAGGGAATCTGGGACTGAGAGGGGGAGGGGAATG24670.1117880929141665No Hit
GAGCAAAAGCAGGCAAAAGTGTCAGAGACAGCCTCTGCTCCCGCTGTTAG24650.11169746616676952No Hit
TCCTTGACCTCTCCTCCCCTCCCTTTCTTTCCCATCCTCCCTCCTTATCT24550.11124433242978467No Hit
GATATAAACAGGGGAGATATAAACAAGGGAGATATAGACTGAATAAATGT24050.10897866374486034No Hit
CCTTATCTCCCTCCATGTGTCTTCCCCTCCCTCACTCTCAGCCCCATCTT22230.10073162973173577No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA31700.0107.776941
GAGCAAA112450.0106.371531
AGCAAAA116800.0101.747362
CAAAAGC132250.089.805984
GCAAAAG142800.083.374253
AAAAGCA154900.076.95515
AGCGAAA50750.066.578942
AAAGCAG233550.065.4762346
GCGAAAG53200.063.3765143
AAGCAGG249150.062.239787
CGAAAGC54450.061.7884254
GCAGGCC28900.059.201449
AGCAGGC86150.057.8963668
GCAGGCA61350.044.195219
AGCAGGT35800.043.7410138
AGCAGGG163050.043.3068168
GGAGCGA5550.040.516591
GCAGGTG29700.038.0790639
ATAACGC1354.970625E-737.5968323
AGTAGAA27300.036.9331471
Produced by FastQC (version 0.11.5)