FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l02_n01_WT_PBS_F1_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l02_n01_WT_PBS_F1_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1896019
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGAGACAGGGAGGGAATCTGGGACTGAGAGGGGGAGGG106160.5599100009018897No Hit
GAGTAGGGGCTGGTGGGAGAAGTGTGTGATGTGGACCATGGTAAAAGCTA55630.2934042327634902No Hit
AAACAAGGTGGAGTAGGGGCTGGTGGGAGAAGTGTGTGATGTGGACCATG47350.24973378431334287No Hit
GAGATAAGGAGGGAGGATGGGAAAGAAAGGGAGGGGAGGAGAGGTCAAGG41100.2167699796257316No Hit
GAGCAAAAGCAGGCCCAAGCAGCCAAAGAGAAGAAGAAGAGGAGGAGGAG40340.21276158097571807No Hit
GGTGAGTGCAAAGGGTTGCAACTGAGCTGCCTTCTGTCATTTATCTAGCT36640.19324700860065222No Hit
GCAAAAGCAGGGAGACAGGGAGGGAATCTGGGACTGAGAGGGGGAGGGGA32120.16940758505057174No Hit
GTAATGGGCCCTGCTCCTTTGTGAGACAAAGGTGAGTGCAAAGGGTTGCA32100.16930210087557138No Hit
AGTAGAAACAAGGTGGAGTAGGGGCTGGTGGGAGAAGTGTGTGATGTGGA30370.1601777197380406No Hit
ATCTGGGACTGAGAGGGGGAGGGGAATGTTTGGAGGCAGAGAGAGAGAGG27850.14688671368799575No Hit
GAGCAAAAGCAGGGGTACCCTTGTAAGAAATTTTCACCACAGAAGGAGCC26760.14113782615047635No Hit
GGTGGGAGAAGTGTGTGATGTGGACCATGGTAAAAGCTACAGTAATGGGC25840.13628555410045998No Hit
GTAGAAACAAGGTGGAGTAGGGGCTGGTGGGAGAAGTGTGTGATGTGGAC25540.13470329147545462No Hit
GAGCGAAAGCAGGGAGACAGGGAGGGAATCTGGGACTGAGAGGGGGAGGG24820.1309058611754418No Hit
AGCAAAAGCAGGGAGACAGGGAGGGAATCTGGGACTGAGAGGGGGAGGGG23830.12568439451292418No Hit
GTGGAGTAGGGGCTGGTGGGAGAAGTGTGTGATGTGGACCATGGTAAAAG22510.1187224389629007No Hit
AGCGAAAGCAGGGAGACAGGGAGGGAATCTGGGACTGAGAGGGGGAGGGG22450.11840598643789962No Hit
AGGTGGAGTAGGGGCTGGTGGGAGAAGTGTGTGATGTGGACCATGGTAAA21620.11402839317538485No Hit
GGGTTGCAACTGAGCTGCCTTCTGTCATTTATCTAGCTTTGCCTTCCCTC21280.11223516220037878No Hit
TCCTTGACCTCTCCTCCCCTCCCTTTCTTTCCCATCCTCCCTCCTTATCT20200.10653901675035957No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA19600.0101.755251
GAGCAAA79900.093.128111
AGCAAAA81800.089.611462
GCAAAAG92750.079.344673
CAAAAGC95650.077.014834
AAAAGCA118300.062.820995
AGCGAAA32150.061.341392
GCGAAAG32250.061.1511763
AGCAGGC55250.059.7049568
CGAAAGC33500.058.869424
AAAGCAG162450.057.664496
AAGCAGG172350.054.347877
GCAGGCC24100.051.741949
CGTGCGA951.0293333E-438.157307145
AGTAGAA22000.037.9101371
GTACGGG800.00203643836.2523082
AGCAGGG123800.036.190888
GTAGAAA28200.035.747521
GCAGGCA42450.034.840459
TAGAAAC29000.034.5021972
Produced by FastQC (version 0.11.5)