FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l02_n01_WT_DI244_F2_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l02_n01_WT_DI244_F2_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3833309
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAA641061.6723410505127556No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG471241.2293295427005755No Hit
AATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA326300.85122279471861No Hit
GAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATA232030.6052994945098347No Hit
TCCTCATTCTGGGCAAAGAGGACAAGAGATATGGGCCAGCACTAAGCATC227230.5927776758930731No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT220910.5762906147143368No Hit
AGCGAAAGCAGGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAAG207740.5419338748845971No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCC191840.5004553507165741No Hit
GAGCGAAAGCAGGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAA182860.4770291150543825No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACATCCCCTTGC181480.47342909220206353No Hit
ATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAAAACCACCGTG178750.4663073078637804No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT174290.4546724513990393No Hit
AAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCCGAAT170890.44580282987883313No Hit
GGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATG166430.4341679734140921No Hit
GCAAAAGCAGGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAAGA163550.42665488224403514No Hit
TCAATGAACTGAGCAACCTTGCGAAAGGAGAGAAGGCTAATGTGCTAATT152720.39840252898996664No Hit
GAATGAGGAATCCCCTCAGTCTTCTCCTGTCTTCCTGATGTGTACTTCTT149450.38987204005729775No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT144410.376724130509698No Hit
AGCAAAAGCAGGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAAG123600.3224368293816126No Hit
GTCTTCTCCTGTCTTCCTGATGTGTACTTCTTGATTATGGCCATATGGTC121770.3176628860339722No Hit
GGAGAAGACTGAGGGGATTCCTCATTCTGGGCAAAGAGGACAAGAGATAT120550.3144802571355453No Hit
GTGCTGGCCCATATCTCTTGTCCTCTTTGCCCAGAATGAGGAATCCCCTC119420.3115324123361826No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG118760.30981066227637793No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA118620.30944544256672235No Hit
GTTCATTGATGCTTAGTGCTGGCCCATATCTCTTGTCCTCTTTGCCCAGA116090.30284540067080423No Hit
GTCTCGCACCCGCGAGATACTCACAAAAACCACCGTGGACCATATGGCCA114870.2996627717723773No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT112600.2937409950515338No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACAT110800.28904531307024817No Hit
ATCAAGAAGTACACATCAGGAAGACAGGAGAAGACTGAGGGGATTCCTCA108460.28294092649457686No Hit
ATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTC108140.2821061385867928No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC108080.28194961585408324No Hit
AGTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATC107790.2811930893126539No Hit
ATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTC98150.2560451035906576No Hit
GGCCAGCACTAAGCATCAATGAACTGAGCAACCTTGCGAAAGGAGAGAAG92690.24180153491409118No Hit
GCTTAGTGCTGGCCCATATCTCTTGTCCTCTTTGCCCAGAATGAGGAATC86260.22502751539205423No Hit
GTTAAGGACAGAAGCCCTTATAGGGCCTTAATGAGCTGCCCTGTCGGTGA86080.22455794719392566No Hit
TCGCAAGGTTGCTCAGTTCATTGATGCTTAGTGCTGGCCCATATCTCTTG85160.22215793195904635No Hit
CCACATCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTCGCAAGG85140.2221057577148098No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA83430.2176448598325885No Hit
GCACTAAGCATCAATGAACTGAGCAACCTTGCGAAAGGAGAGAAGGCTAA82570.2154013673304187No Hit
ATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACC78980.2060360904899657No Hit
ACCAACACCACATCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTT78810.20559260941395543No Hit
GTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCGCTATACCCTG77470.20209693505010945No Hit
GTCCTTAACAGTCCCATTTGAATGCTTGTCATTCAGTAAGGCACCTTGGG76910.20063605621148725No Hit
ATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACATCCCCTTGCCC76000.1982621280987262No Hit
ATATACAGCAAAGACAATAGCATAAGAATTGGTTCCAAAGGAGACGTTTT73070.19061860131807795No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA72550.18926207096792877No Hit
AATTAGCACATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGA71540.18662727163398515No Hit
GTATATAGCCCACCCACGGATGGGACAAAGAGATGAATTGCCGGTTAATA68600.17895765773121866No Hit
CTTCTTGATTATGGCCATATGGTCCACGGTGGTTTTTGTGAGTATCTCGC65620.17118369533997913No Hit
GCCATATGGTCCACGGTGGTTTTTGTGAGTATCTCGCGGGTGCGAGACTG64270.1676619338540149No Hit
CACCACATCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTCGCAA63320.1651836572527808No Hit
CCATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAAGACTG62470.16296625187272928No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC61700.16095754346962377No Hit
CTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGG61670.16087928210326902No Hit
GAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAA61370.1600966684397214No Hit
CTCTTGTCCTCTTTGCCCAGAATGAGGAATCCCCTCAGTCTTCTCCTGTC60850.15874013808957224No Hit
GTACACATCAGGAAGACAGGAGAAGACTGAGGGGATTCCTCATTCTGGGC60060.1566792554422302No Hit
TTCCTGATGTGTACTTCTTGATTATGGCCATATGGTCCACGGTGGTTTTT59290.15467054703912467No Hit
TATCTCTTGTCCTCTTTGCCCAGAATGAGGAATCCCCTCAGTCTTCTCCT58530.15268792575813742No Hit
CATCAGGAAGACAGGAGAAGACTGAGGGGATTCCTCATTCTGGGCAAAGA58500.15260966439178267No Hit
GTGCTAATTGGGCAAGGGGATGTGGTGTTGGTAATGAAACGGAAACGGGA58420.15240096741483664No Hit
CTAGCATACTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGGCC57600.15026182340113983No Hit
CATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGATGCTTAGT57540.15010530066843034No Hit
GTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAA56880.1483835506086256No Hit
GATGTGTACTTCTTGATTATGGCCATATGGTCCACGGTGGTTTTTGTGAG56410.1471574558690677No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA55810.14559222854197248No Hit
GGTATGGTAATGGTGTTTGGATAGGAAGGACCAAAAGTCACAGTTCCAGA55350.14439222092453283No Hit
AAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACATCCCCTT54970.1434009102840392No Hit
TCGCAGTCTCGCACCCGCGAGATACTCACAAAAACCACCGTGGACCATAT54270.14157481173576147No Hit
TAATCAAGAAGTACACATCAGGAAGACAGGAGAAGACTGAGGGGATTCCT51100.1333051940242751No Hit
GCTGTATATAGCCCACCCACGGATGGGACAAAGAGATGAATTGCCGGTTA50840.13262692884920052No Hit
GTATTAAAATACAACGGCATAATAACTGAAACCATAAAAAGTTGGAGGAA50260.13111387576634181No Hit
AAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAAAACCA50150.13082691742304103No Hit
CAATATGGATTAGCCATTCAATTCAAACTGGAAGTCAAAACCATACTGGA49720.12970517117195612No Hit
GGATTAGCCATTCAATTCAAACTGGAAGTCAAAACCATACTGGAATATGC49460.12902690599688155No Hit
GAAGCAGTTTTCTTGATTCAGCTGAAAATCCTTCTAGTTGTGGAGATGCA48930.12764428852461412No Hit
CTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGGCCATCAATTA48320.12605297407540067No Hit
GGCCCATATCTCTTGTCCTCTTTGCCCAGAATGAGGAATCCCCTCAGTCT47480.12386165581746737No Hit
TACCATACCTATATGAAAATCCCTTTACTCCGTTTGCTCCATCAACATAC47260.12328773913086578No Hit
CTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACC47110.122896432299092No Hit
GCCTCATACAGTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCG46460.1212007693614055No Hit
AGATAATGGAGCAGTGGCTGTATTAAAATACAACGGCATAATAACTGAAA46170.12044424281997616No Hit
ACCTTGCGAAAGGAGAGAAGGCTAATGTGCTAATTGGGCAAGGGGATGTG45690.1191920609583No Hit
AGGTTGCTCAGTTCATTGATGCTTAGTGCTGGCCCATATCTCTTGTCCTC45210.11793987909662383No Hit
ATTAGCACATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGAT45070.11757465938696829No Hit
TTTTTGAACAGACTACTTGTCAATGCTGAATGGCAACTCAGCACCGTCTG44880.11707900406672148No Hit
TCTTGATTATGGCCATATGGTCCACGGTGGTTTTTGTGAGTATCTCGCGG43930.11460072746548738No Hit
GAGCAACCTTGCGAAAGGAGAGAAGGCTAATGTGCTAATTGGGCAAGGGG43820.1143137691221866No Hit
GTGTAAATGGTTCATGTTTTACTATAATGACTGATGGCCCGAGTGATGGG43710.11402681077888582No Hit
TCTCCTGTCTTCCTGATGTGTACTTCTTGATTATGGCCATATGGTCCACG42910.11193984100942554No Hit
GGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCG42910.11193984100942554No Hit
ATATAGCCCACCCACGGATGGGACAAAGAGATGAATTGCCGGTTAATATC42860.11180940539883427No Hit
GATTATGGCCATATGGTCCACGGTGGTTTTTGTGAGTATCTCGCGGGTGC42510.1108963561246954No Hit
CTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTCCG42130.10990504548420177No Hit
CCATTATCTGGACCTGAAATTCCGATTGTTAGCCAGCCCATGCCATCATG41800.1090441704542994No Hit
GACTACTTGTCAATGCTGAATGGCAACTCAGCACCGTCTGGCCAAGACCA41510.10828764391287006No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA41420.10805285981380579No Hit
GCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCATTG41130.10729633327237642No Hit
GGGTAAAGGACACAACTTCAGTGATATTAACCGGCAATTCATCTCTTTGT40070.10453109832784155No Hit
AGATATGGGCCAGCACTAAGCATCAATGAACTGAGCAACCTTGCGAAAGG39950.10421805286242251No Hit
GGACAAGAGATATGGGCCAGCACTAAGCATCAATGAACTGAGCAACCTTG39180.102209344459317No Hit
GAAGTACACATCAGGAAGACAGGAGAAGACTGAGGGGATTCCTCATTCTG39160.10215717021508049No Hit
ACCGTGGACCATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGA38840.10132238230729639No Hit
GTCCACGGTGGTTTTTGTGAGTATCTCGCGGGTGCGAGACTGCGACATTA38780.10116585957458686No Hit
CACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGT38720.10100933684187735No Hit
ATATGGGCCAGCACTAAGCATCAATGAACTGAGCAACCTTGCGAAAGGAG38580.1006441171322218No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA26650.0139.61731
GTCCGGT203.860493E-4108.795361
CCGGTAG203.863901E-4108.7712253
GTACGGT203.863901E-4108.7712252
GCAAAAG155350.0100.4042053
GTACGGA301.5967309E-596.7069851
CAAAAGC170400.091.621464
ATTAGTA6500.089.267981
GAGCAAA179450.087.4647141
TACCCCC259.389785E-486.99768145
AGCAAAA182950.085.455322
GCAGGTC151800.083.2915959
GCAGGGG34750.082.4078759
AAAAGCA191100.082.000565
AAGCAGG236050.081.973047
AGCAGGT196400.079.1424948
TATTAGT2150.077.590491
AGCAGGC2250.077.331278
AGCGAAA56250.075.947211
TTAGTAG7800.074.373492
Produced by FastQC (version 0.11.5)