FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l02_n01_WT_Control_F_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l02_n01_WT_Control_F_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2229279
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGAGACAGGGAGGGAATCTGGGACTGAGAGGGGGAGGG273671.2276166419725838No Hit
GAGTAGGGGCTGGTGGGAGAAGTGTGTGATGTGGACCATGGTAAAAGCTA124040.5564130824360701No Hit
AAACAAGGTGGAGTAGGGGCTGGTGGGAGAAGTGTGTGATGTGGACCATG92750.4160538003542849No Hit
GGTGAGTGCAAAGGGTTGCAACTGAGCTGCCTTCTGTCATTTATCTAGCT89750.40259653457463157No Hit
GAGCGAAAGCAGGGAGACAGGGAGGGAATCTGGGACTGAGAGGGGGAGGG73850.3312730259424684No Hit
GCAAAAGCAGGGAGACAGGGAGGGAATCTGGGACTGAGAGGGGGAGGGGA72600.3256658318676128No Hit
GAGATAAGGAGGGAGGATGGGAAAGAAAGGGAGGGGAGGAGAGGTCAAGG68170.3057939360663246No Hit
GTAATGGGCCCTGCTCCTTTGTGAGACAAAGGTGAGTGCAAAGGGTTGCA66260.29722614351994525No Hit
GAGCAAAAGCAGGCCCAAGCAGCCAAAGAGAAGAAGAAGAGGAGGAGGAG64260.2882546330001763No Hit
AGCGAAAGCAGGGAGACAGGGAGGGAATCTGGGACTGAGAGGGGGAGGGG61130.27421421903673787No Hit
AGTAGAAACAAGGTGGAGTAGGGGCTGGTGGGAGAAGTGTGTGATGTGGA59270.2658707142533528No Hit
GGTGGGAGAAGTGTGTGATGTGGACCATGGTAAAAGCTACAGTAATGGGC58240.26125038633567177No Hit
ATCTGGGACTGAGAGGGGGAGGGGAATGTTTGGAGGCAGAGAGAGAGAGG55440.24869027160799523No Hit
AGCAAAAGCAGGGAGACAGGGAGGGAATCTGGGACTGAGAGGGGGAGGGG55420.24860055650279753No Hit
GGGTTGCAACTGAGCTGCCTTCTGTCATTTATCTAGCTTTGCCTTCCCTC51050.22899780601710237No Hit
GTAGAAACAAGGTGGAGTAGGGGCTGGTGGGAGAAGTGTGTGATGTGGAC49270.22101316165450804No Hit
AGGTGGAGTAGGGGCTGGTGGGAGAAGTGTGTGATGTGGACCATGGTAAA46020.20643445705988347No Hit
GTGGAGTAGGGGCTGGTGGGAGAAGTGTGTGATGTGGACCATGGTAAAAG44930.2015449838266094No Hit
TCCTTGACCTCTCCTCCCCTCCCTTTCTTTCCCATCCTCCCTCCTTATCT42840.19216975533345088No Hit
GAGCAAAAGCAGGGGTACCCTTGTAAGAAATTTTCACCACAGAAGGAGCC42060.18867086623074097No Hit
GAGACAGGGAGGGAATCTGGGACTGAGAGGGGGAGGGGAATGTTTGGAGG40690.18252538152469924No Hit
CAGGGAGACAGGGAGGGAATCTGGGACTGAGAGGGGGAGGGGAATGTTTG38940.17467530981990143No Hit
AAAGCAGGGAGACAGGGAGGGAATCTGGGACTGAGAGGGGGAGGGGAATG36630.1643132151695683No Hit
GGTAAAAGCTACAGTAATGGGCCCTGCTCCTTTGTGAGACAAAGGTGAGT35170.15776401249013694No Hit
GAGAGGGGGAGGGGAATGTTTGGAGGCAGAGAGAGAGAGGAAGGGAAGTT34290.1538165478614386No Hit
GGGAGGGAATCTGGGACTGAGAGGGGGAGGGGAATGTTTGGAGGCAGAGA34120.15305396946725824No Hit
CCTTATCTCCCTCCATGTGTCTTCCCCTCCCTCACTCTCAGCCCCATCTT33570.1505868040743218No Hit
GATGTGGACCATGGTAAAAGCTACAGTAATGGGCCCTGCTCCTTTGTGAG31520.1413910057915586No Hit
GAAACAAGGTGGAGTAGGGGCTGGTGGGAGAAGTGTGTGATGTGGACCAT30120.13511094842772034No Hit
GTGGGAGAAGTGTGTGATGTGGACCATGGTAAAAGCTACAGTAATGGGCC30030.13470723045433075No Hit
GACTAAGAGTGGGGAGGGGAGGAAGTGTGGAGGCAGAGAAAGGGAGGGAA29620.1328680707977781No Hit
ATGTGGACCATGGTAAAAGCTACAGTAATGGGCCCTGCTCCTTTGTGAGA28710.12878603351128326No Hit
GGGAGACAGGGAGGGAATCTGGGACTGAGAGGGGGAGGGGAATGTTTGGA26650.11954537767592123No Hit
GAGTGAGGGAGGGGAAGACACATGGAGGGAGATAAGGAGGGAGGATGGGA26280.11788564822976398No Hit
GGTCAAGGAAGGAGGGAAGGCAAAGCTAGATAAATGACAGAAGGCAGCTC25950.11640534899400211No Hit
GATAAGGAGGGAGGATGGGAAAGAAAGGGAGGGGAGGAGAGGTCAAGGAA25890.11613620367840903No Hit
GAGAAAGGGAGGGAAGATGGGGCTGAGAGTGAGGGAGGGGAAGACACATG25440.114117613811461No Hit
GTATAATAACAATCTGAACGGAATCTTAGCTGATGAAATGGGGCTAGGCA25180.11295131744389106No Hit
GAGAGAGAGAGGAAGGGAAGTTGGGACTAAGAGTGGGGAGGGGAGGAAGT24950.11191959373411761No Hit
TGGGAGAAGTGTGTGATGTGGACCATGGTAAAAGCTACAGTAATGGGCCC24350.10922814057818693No Hit
GTTATTATACAGGGAAACCATCCATTCCAGGCCCTGGAGCTGGTAATGCT24180.10846556218400656No Hit
GAGCGAAAGCAGGCCCAAGCAGCCAAAGAGAAGAAGAAGAGGAGGAGGAG23710.10635725721186089No Hit
CAGGGAGGGAATCTGGGACTGAGAGGGGGAGGGGAATGTTTGGAGGCAGA23510.10546010615988398No Hit
AGAGTGGGGAGGGGAGGAAGTGTGGAGGCAGAGAAAGGGAGGGAAGATGG23410.10501153063389554No Hit
GTGTGTGATGTGGACCATGGTAAAAGCTACAGTAATGGGCCCTGCTCCTT23020.10326208608254059No Hit
GAGCAAAAGCAGGCTGCACCACGCTGCACAGCGGCAAAGGATGGTGATGA22620.10146778397858681No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA28050.0121.77561
GAGCAAA118400.0112.397491
AGCAAAA126800.0104.015482
GCAAAAG149000.088.6152043
CAAAAGC150050.087.9467854
AAAAGCA171200.077.251275
AGCGAAA48700.069.5300452
AAAGCAG253650.065.5185556
GCGAAAG52200.064.8680653
AAGCAGG262100.063.370077
CGAAAGC53950.062.8983084
AGCAGGC72550.062.155438
GCAGGCC28200.058.358339
AGTAGAA23900.058.260681
AGCAGGT24000.052.2589848
CGTAATG450.00963659748.3385165
GCAGGGA146100.047.63739
GCAGGCA42450.046.1118139
GGCGAAA803.7293274E-545.3265081
TAGAAAC31000.045.1419333
Produced by FastQC (version 0.11.5)