FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l02_n01_Plasmid_control_HA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l02_n01_Plasmid_control_HA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2704973
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATACATCATGGTCATAAGTTCCATTTCTGATTGACCCTATGCAGGCAT132430.48957974811578525No Hit
GATGTATACAGAGATGAAGCATTAAACAACCGGTTCCAGATCAAAGGTGT99250.3669167862303986No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTCCAAAATAA71040.2626273903658188No Hit
GTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAG69940.2585608063370688No Hit
ATCTGGGACAGGCCCCATATGTGATCCTGTTTACATTTTGAAATGGTTTG61350.2268044819671028No Hit
CATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATC60600.22403181103840963No Hit
GTATTATCCCGTGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACA60220.2226269911012051No Hit
ACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAAT59520.22003916490109143No Hit
TCTAATAACAGTTTCTTTAGTAGATTGAATTGGTTGACCCACTTAAAATT58580.21656408400379598No Hit
CCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTT56400.20850485383772777No Hit
GGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAA54660.20207225728315958No Hit
ATTGTATGTTGGTTCTCCAGGGCAACAAGAAGCTCCGCGTTGTATGACCA54150.2001868410516482No Hit
CTAAAATAGATCTCTGGTCATACAACGCGGAGCTTCTTGTTGCCCTGGAG49530.18310718813089816No Hit
CCCTTACCCTGGGTCTAGATCCGATATTCGGGATTACAGTTTGTTGGCTT49010.18118480295367087No Hit
GTTATTAGATCTCCTTTTGCAAGCAGAGCTTGTTCCATTTTGAGTGACTC48840.18055633087650041No Hit
CATATGGGGCCTGTCCCAGATATGTTAAGCAAAACACTCTGAAATTGGCA48390.17889272831928452No Hit
TCATAAGGGTAACAGTTGCTGTGGGCTTTGCTGCGTTCAACAAAAAGGTC47040.17390192064763677No Hit
TAGTAAAACCGGGAGACATACTTTTGATTAACAGCACAGGGAATCTAATT45930.16979836767317086No Hit
ATTTTAGTGTCCTCAACATATTTCTCGAGGTCCTGAATTCTCCCTTCTAC45530.16831960984453448No Hit
CTATTGGACAATAGTAAAACCGGGAGACATACTTTTGATTAACAGCACAG44900.1659905662644322No Hit
GTCCCAGATATGTTAAGCAAAACACTCTGAAATTGGCAACAGGGATGCGA42980.15889252868697765No Hit
ACATACAATTGATCTAACTGACTCAGAAATGAACAAACTGTTTGAAAGAA42560.15733983296690948No Hit
TCTCAAAAGCACTCAAGCAGCAATCAACCAAATCAATGGGAAGCTGAATA42410.15678529878117084No Hit
GATGAGGACTGTCGCATATTCCACCTGTTGAGGAACTCTGAACCAGCTCA42220.15608288881256857No Hit
GTCCAATAGATGCTTATTCTGCTGGGGATATCCCTTACCCTGGGTCTAGA42150.15582410619255718No Hit
GCATTAATGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGC41230.15242296318669354No Hit
GTCCGTACCCGGGTGGTGAACTCCCCAAATGTACAATTTGTCAAAATTTT40420.14942847858370492No Hit
GAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGG40100.14824547232079582No Hit
CTATTGGGAGACCCTCAGTGTGATGGCTTCCAAAATAAGAAATGGGACCT39150.14473342247778442No Hit
ATGCAGGCATTGTCACATTTGTGGTATATTTTGAAACAACCATTGCCCAT38680.1429958820291367No Hit
CTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGT37940.14026018004615942No Hit
ACATTCTATGTCTGGTTTTCGCTCAAAAACTTCCCGGAAATGACAACAGC37360.1381159811946367No Hit
TCTCTGTATACATCATGGTCATAAGTTCCATTTCTGATTGACCCTATGCA37120.13722872649745485No Hit
CTGTATACATCATGGTCATAAGTTCCATTTCTGATTGACCCTATGCAGGC37080.13708085071459122No Hit
TCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATAC36990.13674813020314805No Hit
CTATTTTAGTGTCCTCAACATATTTCTCGAGGTCCTGAATTCTCCCTTCT36630.13541724815737533No Hit
GGTACGGACAATGACCAAATCAGCCTATATGCTCAAGCATCAGGAAGAAT36210.13386455243730713No Hit
CCCATATCCTCAGCATTTTCCCTCAGTTGCTTCTTTGTTCTTTCAAACAG35690.13194216726007985No Hit
ATACAGAGATGAAGCATTAAACAACCGGTTCCAGATCAAAGGTGTTGAGC35190.1300937199742844No Hit
GTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCT35150.12994584419142075No Hit
ACCCTATGCAGGCATTGTCACATTTGTGGTATATTTTGAAACAACCATTG34720.12835617952563666No Hit
GTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACT33570.1241047507683071No Hit
TTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGT33540.12399384393115939No Hit
GTTTTACTATTGTCCAATAGATGCTTATTCTGCTGGGGATATCCCTTACC33360.12332840290827302No Hit
TGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATC32960.12184964507963665No Hit
ATCTATTAGTGTGCAGTTTTCTCCATCAAGGATCTGATGAGGACTGTCGC32800.12125814194818212No Hit
GTCCTGGGGTTGACCAGAGGGCCCCGGGCGCTCCGTGTGTGGCTGCGATG31530.11656308584226163No Hit
CTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCA31520.11652611689654574No Hit
ACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATA30930.1143449490993071No Hit
ATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAAT30900.11423404226215937No Hit
GGGTAAGGGATATCCCCAGCAGAATAAGCATCTATTGGACAATAGTAAAA30870.11412313542501164No Hit
GTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAATGTTGCCTTT30840.1140122285878639No Hit
TTCTATGTCTGGTTTTCGCTCAAAAACTTCCCGGAAATGACAACAGCACG30780.11379041491356846No Hit
GTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGT30460.11260740865065937No Hit
CTATTAACCATGAAGACTATCTTTGCTTTGAGCTACATTCTATGTCTGGT30420.11245953286779573No Hit
GGTATATCTTTCGCTCCGAGTCGGCATTTTGGGCCGCCGGGTTATTAGTA30260.11186802973634118No Hit
GCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAG30130.11138743344203436No Hit
AACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAG29970.1107959303105798No Hit
TATGGGGCCTGTCCCAGATATGTTAAGCAAAACACTCTGAAATTGGCAAC29960.1107589613648639No Hit
GCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCA29880.11046320979913662No Hit
CCCTTATGATGTGCCGGATTATGCCTCCCTTAGGTCACTAGTTGCCTCAT29670.10968686193910254No Hit
ATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTG29400.10868870040477299No Hit
ATGTGATCCTGTTTACATTTTGAAATGGTTTGTCATTGGGAATGCTTCCA29350.10850385567619344No Hit
GTCATTGGGAATGCTTCCATTTGGAGTGATGCATTCAGAATTGCATTTGC29210.10798629043617071No Hit
TCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTG28980.10713600468470481No Hit
GAATAGGACAAGCAGCAGATCTCAAAAGCACTCAAGCAGCAATCAACCAA28970.1070990357389889No Hit
ATTGGGAGACCCTCAGTGTGATGGCTTCCAAAATAAGAAATGGGACCTTT28770.1063596568246707No Hit
ATTAGATTCCCTGTGCTGTTAATCAAAAGTATGTCTCCCGGTTTTACTAT28700.10610087420465934No Hit
CACTAAAATAGATCTCTGGTCATACAACGCGGAGCTTCTTGTTGCCCTGG28630.10584209158464798No Hit
CAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCG28370.10488089899603434No Hit
GTCCTATTCCCTCAGAATTTTGATGCCTGAAACCGTACCAACCATCCACC28360.10484393005031843No Hit
TTTGGGGAGTTCACCACCCGGGTACGGACAATGACCAAATCAGCCTATAT28320.10469605426745479No Hit
GTCCTGAATTCTCCCTTCTACTTCTGAGAATTCTTTTTCAATCTGATGGA28320.10469605426745479No Hit
TTCTTTAGTAGATTGAATTGGTTGACCCACTTAAAATTCAAATACCCAGC28250.10443727164744343No Hit
GATCTAGACCCAGGGTAAGGGATATCCCCAGCAGAATAAGCATCTATTGG28170.10414152008171615No Hit
TACTAATGCTACTGAGCTGGTTCAGAGTTCCTCAACAGGTGGAATATGCG27970.10340214116739799No Hit
ACTAAAGAAACTGTTATTAGATCTCCTTTTGCAAGCAGAGCTTGTTCCAT27760.10262579330736388No Hit
GTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCAT27590.10199732123019342No Hit
TTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATT27270.10081431496728434No Hit
GATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGA27270.10081431496728434No Hit
GTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAG27120.1002597807815457No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGGA3350.071.42382
TTCGGAC3650.065.553353
CGGACAT3750.063.805265
CTTTCGG3850.062.167521
GGACATC4200.062.1479846
TCGGACA3850.062.147984
GACATCT4250.057.9983527
AGAGTGG3350.051.9445845
GAGTGGC3650.049.661636
GTCCAGA3600.048.3525161
ATAAGAG657.335828E-444.6190643
CCAGAGT4050.042.9665073
AGTGGCC4150.041.9265177
GAGCCCC2650.038.300797145
CCGCTAG2600.036.252992
TATAAGA1001.391401E-436.252992
CTGGACT4100.035.368774
GTAATAC850.00274169934.120463
CATGGTC57250.034.0645379
GCTAGCT2800.033.663494
Produced by FastQC (version 0.11.5)