FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l02_n01_PR8_P5TC_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l02_n01_PR8_P5TC_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3343791
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG252250.7543832733564987No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC140140.419105141439761No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT120420.3601301636376197No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA118910.3556143311588553No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA102420.3062990479967199No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT95100.2844077276360873No Hit
AGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGC85410.2554286437160696No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA83370.24932778394343427No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT81360.2433166426968671No Hit
GATGTAAGGCTTGCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATC73130.2187038603788335No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA71080.2125730944308421No Hit
GCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGCT70120.2097021015966608No Hit
GTAGAAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTT68290.20422927150650266No Hit
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT68280.20419936533114658No Hit
GGTCCATGCTCATACCCAAGCAGAAAGTGGCAGGCCCTCTTTGTATCAGA66410.19860691053956422No Hit
TGATAACACAGTTCGAGTCTCTGAAACTCTACAGAGATTCGCTTGGAGAA66380.19851719201349605No Hit
GACATAATGGATCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCT66140.1977994438049507No Hit
CCTCAGCAGTATGTCCTGGAAGAGAAGGCAATGGTGAAATTTCGCCAACA62690.1874818133071116No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA61950.18526875633076348No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG61700.1845211019468621No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT60920.1821884202690898No Hit
GAGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG58720.1756090616907576No Hit
AGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGC55320.16544096206969874No Hit
GAGTGGAGGTCTCCCATTCTCATTACTGCTTCTCCAAGCGAATCTCTGTA53770.16080550488951015No Hit
GAATGGACCAGGCGATCATGGATAAGAACATCATACTGAAAGCGAACTTC53130.15889150966672258No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG52540.15712704532071534No Hit
CTCTTGCTCCACTTCAAGCAATAGATGTAAGGCTTGCATAAATGTTATTT51270.15332896105049626No Hit
TCTCTGTAGAGTTTCAGAGACTCGAACTGTGTTATCATTCCATTCAAGTC50730.15171402758126928No Hit
GATACAAAGAGGGCCTGCCACTTTCTGCTTGGGTATGAGCATGGACCAGT50400.15072712379451944No Hit
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCTGCTGT49910.14926172120207273No Hit
GATAAGAACATCATACTGAAAGCGAACTTCAGTGTGATTTTTGACCGGCT46830.14005061919240766No Hit
GTTTTGGAGTGAGTGGAGGTCTCCCATTCTCATTACTGCTTCTCCAAGCG46830.14005061919240766No Hit
GTCGTACTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA46720.13972165126349106No Hit
TCCTAGCTCCAGTGCTGGTCTGAAAAATGATCTTCTTGAAAATTTGCAGG46630.13945249568528656No Hit
TCTCATTACTGCTTCTCCAAGCGAATCTCTGTAGAGTTTCAGAGACTCGA46030.13765812516392323No Hit
CTTTGTATCAGAATGGACCAGGCGATCATGGATAAGAACATCATACTGAA45710.13670112755252944No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCCCCGTTCCC45620.13643197197432494No Hit
CTGCTGAGGATGTCAAAAATGCAGTTGGAGTCCTCATCGGGGGACTTGAA45570.13628244109754467No Hit
GTTCTTATCTCTTGCTCCACTTCAAGCAATAGATGTAAGGCTTGCATAAA45500.13607309787005228No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT45260.13535534966150695No Hit
ACCTAACTGACATGACTCTTGAGGAAATGTCAAGGGACTGGTCCATGCTC44220.13224510742447718No Hit
ATGTAAGGCTTGCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATCT44180.13212548272305297No Hit
GGTACAGAGGCCATGGTCATTTTAAGTGCCTCATCGGATTCTTCTTTCAG43850.13113857893630312No Hit
GGTATGTGCAACCTGTGAACAGATTGCTGACTCCCAGCATCGGTCTCATA43760.13086942335809865No Hit
CTACAGAGATTCGCTTGGAGAAGCAGTAATGAGAATGGGAGACCTCCACT43530.13018158132490937No Hit
GGTCCATTCTGATACAAAGAGGGCCTGCCACTTTCTGCTTGGGTATGAGC43350.12964327016850036No Hit
CTGTACCTGCGTCGCGTTACCTAACTGACATGACTCTTGAGGAAATGTCA42250.12635359087933426No Hit
TCTTATTTCTTCAAACTTCTGACCTAATTGTTCCCGCCATTTCTCGTTTC41750.1248582821115315No Hit
AAACTATTCTCTGTTATCTTCAGTTTGTGTCTCACTTCTTCAATCAACCA41410.1238414721494256No Hit
CCTGTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGAT40740.12183775840056989No Hit
GTTCTTATCCATGATCGCCTGGTCCATTCTGATACAAAGAGGGCCTGCCA40720.12177794604985778No Hit
CTTCCAGGACATACTGCTGAGGATGTCAAAAATGCAGTTGGAGTCCTCAT40500.12112001019202456No Hit
GCATGGACCAGTCCCTTGACATTTCCTCAAGAGTCATGTCAGTTAGGTAA39980.11956488907350966No Hit
GAACTAGGTGATGCCCCATTCCTTGATCGGCTTCGCCGAGATCAGAAATC39110.11696305181753285No Hit
CCTATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTA38480.11507896277010138No Hit
GTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGTATGATGTT38140.11406215280799549No Hit
CTCCAAAACAGAAACGAGAAATGGCGGGAACAATTAGGTCAGAAGTTTGA36860.11023416236242038No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAAACCTACT36470.10906782152353423No Hit
TCCTGGAAGAGAAGGCAATGGTGAAATTTCGCCAACAATTGCTCCCTCTT35810.10709401395003455No Hit
GTCCTGGAAGAGAAGGCAATGGTGAAATTTCGCCAACAATTGCTCCCTCT35700.10676504602111796No Hit
GTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCCGCAAACGAGT35520.10622673486470895No Hit
CATTACTGCTTCTCCAAGCGAATCTCTGTAGAGTTTCAGAGACTCGAACT35250.10541926813009544No Hit
AGTAGAAACAAGGTAGTTTTTTACTCCAGCTCTATGCTGACAAAATGACC35070.10488095697368646No Hit
TCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCCCCGTTCCCA34830.10416320876514114No Hit
AAACAAGGTAGTTTTTTACTCCAGCTCTATGCTGACAAAATGACCATCGT34750.10392395936229268No Hit
AGCATGGACCAGTCCCTTGACATTTCCTCAAGAGTCATGTCAGTTAGGTA34630.10356508525802002No Hit
CTTAAAATGACCATGGCCTCTGTACCTGCGTCGCGTTACCTAACTGACAT34240.10239874441913385No Hit
CTCTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCACAGAGACTTG34140.10209968266557329No Hit
CTGTTATCTTCAGTTTGTGTCTCACTTCTTCAATCAACCATCTTATTTCT34090.10195015178879302No Hit
CTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCACAGAGACTTGAA33950.10153146533380825No Hit
CTCTCTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCACAGAGACT33880.10132212210631586No Hit
GTCCATGCTCATACCCAAGCAGAAAGTGGCAGGCCCTCTTTGTATCAGAA33730.10087352947597503No Hit
GTATGATGTTCTTATCCATGATCGCCTGGTCCATTCTGATACAAAGAGGG33580.10042493684563418No Hit
GTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGAGTG33560.10036512449492208No Hit
ATGATGTTCTTATCCATGATCGCCTGGTCCATTCTGATACAAAGAGGGCC33500.10018568744278576No Hit
CTCATAGACTTTGGCACTCCTTCCGTAGAAGGCCCTCCTTTCAGTCCGTA33460.10006606274136153No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACG100.007092199144.99591145
GAGCGAA12600.0136.430481
GCAAAAG79800.099.1405943
AGCAAAA81850.096.746132
ACTAATA2550.093.820888
AGCAGGC6300.092.060898
ATTAGTA3900.083.691531
GCAGGGG13500.082.1643459
AGCGAAA28750.079.9751361
GCAGGGT73550.077.672869
AGCAGGG89700.076.0541468
TACTAAT3400.070.365667
CAAAAGC115950.068.794164
TATTAGT1500.067.697151
AAGCAGG145450.066.3920757
CTACTAA3650.065.5598146
GGTACTT451.1944333E-464.442627
AAGGGTG30550.063.1393475
TCTACTA3800.062.9738125
AGGGTGT31250.062.1872636
Produced by FastQC (version 0.11.5)