FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l02_n01_No_index_control.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l02_n01_No_index_control.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAA65.607476635514018No Hit
AATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA32.803738317757009No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT21.8691588785046727No Hit
ATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACC21.8691588785046727No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT21.8691588785046727No Hit
ATAGTAAGTTCTCTGTTAGGCAAGATGTTGTGGCAATGACTGATTGGTCA10.9345794392523363No Hit
GCTGTATATAGCCCACCCACGGATGGGACAAAGAGATGAATTGCCGGTTA10.9345794392523363No Hit
TACCAACACCACATCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTT10.9345794392523363No Hit
TCCATTAGGATCCCAAATCATCTCAAACCCATGTCTGGAACTGTGACTTT10.9345794392523363No Hit
CCCTTAGTCAGAGGTGACAGGATTGGTCTTGTCTTTAGCCATTCCATGAG10.9345794392523363No Hit
AATATGCACTCACTTGGAAGTATGCTTCATGTATTCAGATTTTCAGAAGG10.9345794392523363No Hit
AGCATAAGAATTGGTTCCAAAGGAGACGTTTTTGTCATAAGAGAGCCCTT10.9345794392523363No Hit
TGTGCTAATTGGGCAAGGGGATGTGGTGTTGGTAATGAAACGGAAACGGG10.9345794392523363No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACATCCCCTTGC10.9345794392523363No Hit
CAATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTAATGCTTCT10.9345794392523363No Hit
GGATACATCTGCAGTGGGGTTTTCGGTGACAACCCGCGTCCCGAAGATGG10.9345794392523363No Hit
CTTAGGGACAACCTTGAACCTGGGACCTTTGATCTTGGGGGGCTATATGA10.9345794392523363No Hit
TAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAA10.9345794392523363No Hit
ACTTATCAGAGGACAAGAGCTCTTGTTCGCACCGGAATGGATCCCAGGAT10.9345794392523363No Hit
GCAAAAGCAGGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAAGA10.9345794392523363No Hit
GTGTAAATGGTTCATGTTTTACTATAATGACTGATGGCCCGAGTGATGGG10.9345794392523363No Hit
CTCTTGTGTCCTCAATATTTTCTTCCTCCAACTTTTTATGGTTTCAGTTA10.9345794392523363No Hit
TTAGTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCA10.9345794392523363No Hit
CTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAG10.9345794392523363No Hit
AGGCAGCTGTGGTCCAGTGTATGTTGATGGAGCAAACGGAGTAAAGGGAT10.9345794392523363No Hit
GATTTTAGGATTTGTGTTCACGCTCACCGTGCCCAGTGAGCGAGGACTGC10.9345794392523363No Hit
GGAGATATGCTTATAAGAAGTGCCATAGGCCAGGTTTCAAGGCCCATGTT10.9345794392523363No Hit
TTCCATGAGAACCTCAAGATCAGTGTTCTTCCCTGCAAAGACATCTTCAA10.9345794392523363No Hit
TCAGTCATTGCCACAACATCTTGCCTAACAGAGAACTTACTATCAGTCTC10.9345794392523363No Hit
GCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCATTG10.9345794392523363No Hit
GACATGCACTTGCTGACCAAGCAACCGATTCAAATCTTAAATTGTACGGG10.9345794392523363No Hit
ACATCCCATGGGGCCAAAGAAATCTCACTCAGTTATTCTGCTGGTGCACT10.9345794392523363No Hit
CTATAATGACTGATGGCCCGAGTGATGGGCTGGCCTCGTACAAAATTTTC10.9345794392523363No Hit
AGGCTATGGAGCAAATGGCTGGATCGAGTGAGCAAGCAGCAGAGGCCATG10.9345794392523363No Hit
AAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACATCCCCTT10.9345794392523363No Hit
CTGTTAAGGACAGAAGCCCTTATAGGGCCTTAATGAGCTGCCCTGTCGGT10.9345794392523363No Hit
GTTGCAGACCAAGAACTAGGTGATGCCCCATTCCTTGATCGGCTTCGCCG10.9345794392523363No Hit
TCAGAGACTCGAACTGTGTTATCATTCCATTCAAGTCCCCCGATGAGGAC10.9345794392523363No Hit
ACTGCTATCCTGTTCCTGTCCCATGGCTGTAAGGAGGGTCTCCAGTATAA10.9345794392523363No Hit
CTCCTCTCAGTCCGTATTTAAAGCGACGGTAAATGCATTTGAAAAAAAGA10.9345794392523363No Hit
GGACTTCTTTCCCTTTTTTGTTCACATAAGAATTTTTCAGCTTTGGGTAT10.9345794392523363No Hit
ATAGCGAGAGGATCACTTGAACCGTTGCATCTGCACCCCCATTCGTTTCT10.9345794392523363No Hit
CCTATATGAAAATCCCTTTACTCCGTTTGCTCCATCAACATACACTGGAC10.9345794392523363No Hit
TTCCTGATGTGTACTTCTTGATTATGGCCATATGGTCCACGGTGGTTTTT10.9345794392523363No Hit
TTACTAAATCAATAGAGTTGAATGCACCTAATTCTCACTATGAGGAATGT10.9345794392523363No Hit
GATGTATCCTATTTGATAATCCAGGTTTTGATCGAAAGACACCCATGGCC10.9345794392523363No Hit
TACATCTGCAGTGGGGTTTTCGGTGACAACCCGCGTCCCGAAGATGGAAC10.9345794392523363No Hit
TTGCAATGTGTTCAATTGGAGCCACATCTTCTCCAATCTCATCGAGCTCT10.9345794392523363No Hit
GGACACAAGAGTCTGAATGTGCCTGTGTAAATGGTTCATGTTTTACTATA10.9345794392523363No Hit
GAGGGAATACCGCTATATGATGCAATCAAATGCATGAGAACATTCTTTGG10.9345794392523363No Hit
GTCTGAATGTGCCTGTGTAAATGGTTCATGTTTTACTATAATGACTGATG10.9345794392523363No Hit
GTATGAGGCCGTGCTTCTGGGTTGAATTAATCAGGGGACGACCTAAAGAA10.9345794392523363No Hit
GCCTGATCCCTGTTCATTCTGATGATGATAACCATACCATCCATCTATCA10.9345794392523363No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA10.9345794392523363No Hit
TGCAATATTAGGCTAATTAGTCCGACTACCAGACAGATTGATCCAATGGT10.9345794392523363No Hit
ATATGGATTAGCCATTCAATTCAAACTGGAAGTCAAAACCATACTGGAAT10.9345794392523363No Hit
GTGTTGCAAATACTGTTTACTACTGTCCAGGCCATTGTGCGATCTCTTCC10.9345794392523363No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG10.9345794392523363No Hit
GTCTCTGCACACACACATCACTTTGCCGGTATCAGGGTAACAGGAACATT10.9345794392523363No Hit
GATCTTCATGATCTCAGTGAACTCTTCTTTCTTTATCCTTCCAGATTCGA10.9345794392523363No Hit
TCATTAATCAGGCACTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAA10.9345794392523363No Hit
CAGCATCCACAGCACTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGAC10.9345794392523363No Hit
TTCTGATACAAAGAGGGCCTGCCACTTTCTGCTTGGGTATGAGCATGGAC10.9345794392523363No Hit
GGCCTGGTATGTGCAACCTGTGAACAGATTGCTGACTCCCAGCATCGGTC10.9345794392523363No Hit
TTATATTCCCTATTTGCAATATTAGGCTAATTAGTCCGACTACCAGACAG10.9345794392523363No Hit
CGGGGACGGAGCTTCACCGACAGGGCAGCTCATTAAGGCCCTATAAGGGC10.9345794392523363No Hit
ATTTTAAGGCATCCATCAGCAGGAATTTGGACCGCTGAGAACAGGGAGGC10.9345794392523363No Hit
ACAATGAAAGAGTATGGGGAGGACCTGAAAATCGAAACAAACAAATTTGC10.9345794392523363No Hit
GAGTGCATATTGCTGCAAATTTGTTTGTTTCGATTTTCAGGTCCTCCCCA10.9345794392523363No Hit
TACCCTGACCAATCAGTCATTGCCACAACATCTTGCCTAACAGAGAACTT10.9345794392523363No Hit
TAGTCTGACTAATTAGCCTAATATTGCAAATAGGGAATATAATCTCAATA10.9345794392523363No Hit
ATTCTGGGCAAAGAGGACAAGAGATATGGGCCAGCACTAAGCATCAATGA10.9345794392523363No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAACTCTTTTGGTCG10.9345794392523363No Hit
TACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCCGATGAT10.9345794392523363No Hit
GTTTTGGAGTGAGTGGAGGTCTCCCATTCTCATTACTGCTTCTCCAAGCG10.9345794392523363No Hit
TCTCCTATCTCAAGAACACAGTACTTCTCCCATTTATGTGGTTCAAGTCT10.9345794392523363No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG10.9345794392523363No Hit
TCTCAAACCCATGTCTGGAACTGTGACTTTTGGTCCTTCCTATCCAAACA10.9345794392523363No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG10.9345794392523363No Hit
GAATGTGCCTGTGTAAATGGTTCATGTTTTACTATAATGACTGATGGCCC10.9345794392523363No Hit
AAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCCGAAT10.9345794392523363No Hit
CTCACAAAAACCACCGTGGACCATATGGCCATAATCAAGAAGTACACATC10.9345794392523363No Hit
CCCATGGCTGTAAGGAGGGTCTCCAGTATAAGGGAAAGTTGTGCTTATAG10.9345794392523363No Hit
GGTGTATGTTGATGGAGCAAACGGAGTAAAGGGATTTTCATATAGGTATG10.9345794392523363No Hit
CTTGATTATGGCCATATGGTCCACGGTGGTTTTTGTGAGTATCTCGCGGG10.9345794392523363No Hit
CTAAATCAATAGAGTTGAATGCACCTAATTCTCACTATGAGGAATGTTCC10.9345794392523363No Hit
GATATTAACCGGCAATTCATCTCTTTGTCCCATCCGTGGGTGGGCTATAT10.9345794392523363No Hit
GTCTGATTAGTGGATTGGTTGTTGTCACCATTTGCCTATGAGACCGATGC10.9345794392523363No Hit
ATCAAGAAGTACACATCAGGAAGACAGGAGAAGACTGAGGGGATTCCTCA10.9345794392523363No Hit
CCCATATCTCTTGTCCTCTTTGCCCAGAATGAGGAATCCCCTCAGTCTTC10.9345794392523363No Hit
CTGTAGTGTTGCAAATACTGTTTACTACTGTCCAGGCCATTGTGCGATCT10.9345794392523363No Hit
ATATAGCCCACCCACGGATGGGACAAAGAGATGAATTGCCGGTTAATATC10.9345794392523363No Hit
AAAATGATCTTCTTGAAAATTTGCAGGCCTATCAGAAACGAATGGGGGTG10.9345794392523363No Hit
GGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATG10.9345794392523363No Hit
GACAGAGACTGATAGTAAGTTCTCTGTTAGGCAAGATGTTGTGGCAATGA10.9345794392523363No Hit
ATATGGTCCACGGTGGTTTTTGTGAGTATCTCGCGGGTGCGAGACTGCGA10.9345794392523363No Hit
GGTGTTCTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTATCTCTTG10.9345794392523363No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers