FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l02_n01_MRTPCR_NC.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l02_n01_MRTPCR_NC.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7217
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGAAAGCAGGCCGGCCTGCTGCCGCGCGACTACCCGGTGAGCATCCAC500.6928086462519053No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT480.665096300401829No Hit
GGGATGAATGCCGCGGCGGGCGACGATAGGGCCTGAAATCGGCTAAAGGT400.5542469170015242No Hit
GTCTCCGGTTACTCGGGAGGCGGAAGGGCCGCCGTCGAAGCATTCGAATC350.4849660523763337No Hit
GATGAATGCCGCGGCGGGCGACGATAGGGCCTGAAATCGGCTAAAGGTGA340.47110987945129557No Hit
AGCGAAAGCAGGGATGAATGCCGCGGCGGGCGACGATAGGGCCTGAAATC340.47110987945129557No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT250.34640432312595265RNA PCR Primer, Index 27 (95% over 23bp)
AAAGCAGGCCGGCCTGCTGCCGCGCGACTACCCGGTGAGCATCCACGCGG250.34640432312595265No Hit
AAGCAGGGATGAATGCCGCGGCGGGCGACGATAGGGCCTGAAATCGGCTA240.3325481502009145No Hit
GCGTTCGACAGTCCGAGCATCAAGTCGAGATTCTGCACGGCCGCACCGGA230.3186919772758764No Hit
TATCAAGACGGTGCTCTAACCGACTGAGCTACAGACCCCTGAGTCTGTCT230.3186919772758764No Hit
AGCAAAAGCAGGCCGGCCTGCTGCCGCGCGACTACCCGGTGAGCATCCAC230.3186919772758764No Hit
GGCTAAAGGTGAGCTTTTACGGGGGGCGTGTGCAGGCACCGGCGCGACGC230.3186919772758764No Hit
CTCTAAGGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCA220.3048358043508383No Hit
CCACTACGCCGGCGCGCGTCACGTCGACGTCACGCCGCTGGCTGACGCGC210.2909796314258002No Hit
GGCGACGATAGGGCCTGAAATCGGCTAAAGGTGAGCTTTTACGGGGGGCG190.263267285575724No Hit
CCTGGGGAGTACGGTCGCAAGATTAAAACTCAAAGGAATTGACGGGGACC190.263267285575724No Hit
CCGTAGGCCGGCACGAAGAACGGGCGGTGTGCGAGCCCGGCATGCAGCCG190.263267285575724No Hit
AGATACGTGATTGCGTGCGCGTCAGCCAGCGGCGTGACGTCGACGTGACG180.24941111265068586No Hit
GCAGGGATGAATGCCGCGGCGGGCGACGATAGGGCCTGAAATCGGCTAAA180.24941111265068586No Hit
GGCCTACGGTGCCTATCGGCAAGGCATCGTGCTCACCGTGCCGATCGAGC180.24941111265068586No Hit
CACGTATCTCGATCCGCAAGCGCTGAACGGCACCAACGACCTGCGGCTCG170.23555493972564778No Hit
GGTTTAGGATGCTCGCAAACGCCAAGGCGCTTACAACCACCAACACGTCA170.23555493972564778No Hit
CTTCATTGACGAACACGCCGAGCCGCAGGTCGTTGGTGCCGTTCAGCGCT170.23555493972564778No Hit
TTTACGGGGGGCGTGTGCAGGCACCGGCGCGACGCGGATCGGCCTGCCGG160.22169876680060968No Hit
CCCTGGTAGTCCACGCCCTAAACGATGTCAACTAGTTGTTGGGGATTCAT160.22169876680060968No Hit
GTCTAACCGCAAGGAGGACGGTCACCACGGTAGGATTCATGACTGGGGTG160.22169876680060968No Hit
TGTACACACCGCCCGTCACACCATGGGAGTGGGTTTTACCAGAAGTGGCT160.22169876680060968No Hit
ATTCTGCACGGCCGCACCGGACGCCCCTTTGCCGAGATTGTCGAACACGG160.22169876680060968No Hit
CCCTTGACATGGTCGGAATTCCGCTGAGAGGTGGAAGTGCTCGAAAGAGA150.20784259387557155No Hit
GTCCGAGCATCAAGTCGAGATTCTGCACGGCCGCACCGGACGCCCCTTTG150.20784259387557155No Hit
GGCGGAAGGGCCGCCGTCGAAGCATTCGAATCCGGCGACGCCACCGCACG140.19398642095053348No Hit
TGTCAAGGGTAGGTAAGGTTTTTCGCGTTGCATCGAATTAATCCACATCA140.19398642095053348No Hit
CTCGCACACCGCCCGTTCTTCGTGCCGGCCTACGGTGCCTATCGGCAAGG140.19398642095053348No Hit
TCATTGACGAACACGCCGAGCCGCAGGTCGTTGGTGCCGTTCAGCGCTTG140.19398642095053348No Hit
GACGAACACGCCGAGCCGCAGGTCGTTGGTGCCGTTCAGCGCTTGCGGAT140.19398642095053348No Hit
TAGCTAACGCGTGAAGTTGACCGCCTGGGGAGTACGGTCGCAAGATTAAA140.19398642095053348No Hit
ATGTACAGGTATCCTCAGGATTCCAGCCTGTCGGTTTAGGATGCTCGCAA140.19398642095053348No Hit
TAGTAACGTAGCTAACGCGTGAAGTTGACCGCCTGGGGAGTACGGTCGCA140.19398642095053348No Hit
CCCGGTGAGCATCCACGCGGTCTCCGGTTACTCGGGAGGCGGAAGGGCCG140.19398642095053348No Hit
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTG140.19398642095053348RNA PCR Primer, Index 27 (96% over 30bp)
AGCATGTTCCCGAGATCCGGCTGCATGCCGGGCTCGCACACCGCCCGTTC130.18013024802549535No Hit
ACCCGGTGAGCATCCACGCGGTCTCCGGTTACTCGGGAGGCGGAAGGGCC130.18013024802549535No Hit
GTATCGGAAGGTGCGGCTGGATCACCTCCTTTCCAGAGCTTCTCGCAAAG130.18013024802549535No Hit
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCT130.18013024802549535RNA PCR Primer, Index 27 (96% over 25bp)
GTGCTCACCGTGCCGATCGAGCTGCGCCTGCTTCCGGCCGGCGTGACCGG130.18013024802549535No Hit
GAGTTGCAGAGTGCAATCCGGACTACGATCGGTTTTCTGGGATTAGCTCC130.18013024802549535No Hit
TCATACAATGGTCGGAACAGAGGGTTGCCAACCCGCGAGGGGGAGCTAAT130.18013024802549535No Hit
TCCTAAACCGACAGGCTGGAATCCTGAGGATACCTGTACATGGGGGCATA130.18013024802549535No Hit
AGCGAAAGCAGGTGCTCTCCCAGCTGAGCTATGCCCCCATGTACAGGTAT130.18013024802549535No Hit
GTCCCGCAACGAGCGCAACCCTTGTCCTTAGTTGCTACGCAAGAGCACTC120.16627407510045725No Hit
TTCATTTCCTTAGTAACGTAGCTAACGCGTGAAGTTGACCGCCTGGGGAG120.16627407510045725No Hit
GCCTGCTGCCGCGCGACTACCCGGTGAGCATCCACGCGGTCTCCGGTTAC120.16627407510045725No Hit
TCAATGAAGAACGCGGACAGGTGTTGCTGTCCGCCGTGTTCGACAATCTC120.16627407510045725No Hit
GCGTAGTGGTGCGCGAGACACGCATGCAGCTGCTCACCGGTCACGCCGGC120.16627407510045725No Hit
TCAGCCAGCGGCGTGACGTCGACGTGACGCGCGCCGGCGTAGTGGTGCGC110.15241790217541915No Hit
AGCTGCGCCTGCTTCCGGCCGGCGTGACCGGTGAGCAGCTGCATGCGTGT110.15241790217541915No Hit
CCTTAGTAACGTAGCTAACGCGTGAAGTTGACCGCCTGGGGAGTACGGTC110.15241790217541915No Hit
GAGCTACAGACCCCTGAGTCTGTCTTTAATTTACAGCCGATAAGCGTGAG110.15241790217541915No Hit
GCCTGGCGCTGGAGCACAAGCATGTTCCCGAGATCCGGCTGCATGCCGGG110.15241790217541915No Hit
CTGCACGGCCGCACCGGACGCCCCTTTGCCGAGATTGTCGAACACGGCGG110.15241790217541915No Hit
CCCTAAACGATGTCAACTAGTTGTTGGGGATTCATTTCCTTAGTAACGTA110.15241790217541915No Hit
CCGGTTACTCGGGAGGCGGAAGGGCCGCCGTCGAAGCATTCGAATCCGGC110.15241790217541915No Hit
CCCCTTTGCCGAGATTGTCGAACACGGCGGACAGCAACACCTGTCCGCGT110.15241790217541915No Hit
TTCCAGCTTCATGCACTCGAGTTGCAGAGTGCAATCCGGACTACGATCGG110.15241790217541915No Hit
TGCGTGTCTCGCGCACCACTACGCCGGCGCGCGTCACGTCGACGTCACGC110.15241790217541915No Hit
CCCATGGTGTGACGGGCGGTGTGTACAAGACCCGGGAACGTATTCACCGC110.15241790217541915No Hit
ATTCCAGCCTGTCGGTTTAGGATGCTCGCAAACGCCAAGGCGCTTACAAC110.15241790217541915No Hit
TTCCAGAGCTTCTCGCAAAGTTGAGCGCTCACGCTTATCGGCTGTAAATT110.15241790217541915No Hit
AAGCAGGCCGGCCTGCTGCCGCGCGACTACCCGGTGAGCATCCACGCGGT110.15241790217541915No Hit
CCTTAGAGTGCTCTTGCGTAGCAACTAAGGACAAGGGTTGCGCTCGTTGC100.13856172925038104No Hit
CTCGGGAGGCGGAAGGGCCGCCGTCGAAGCATTCGAATCCGGCGACGCCA100.13856172925038104No Hit
GTGTACGGCCTGGCGCTGGAGCACAAGCATGTTCCCGAGATCCGGCTGCA100.13856172925038104No Hit
GTGTGCGAGCCCGGCATGCAGCCGGATCTCGGGAACATGCTTGTGCTCCA100.13856172925038104No Hit
ACCCTTGACATGGTCGGAATTCCGCTGAGAGGTGGAAGTGCTCGAAAGAG100.13856172925038104No Hit
TTCGAATCCGGCGACGCCACCGCACGCGTGCTGCCGCTGCAGGTGTACGG100.13856172925038104No Hit
GGCTCGCACACCGCCCGTTCTTCGTGCCGGCCTACGGTGCCTATCGGCAA100.13856172925038104No Hit
AGCGAAAGCAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCA100.13856172925038104No Hit
ACGTGATTGCGTGCGCGTCAGCCAGCGGCGTGACGTCGACGTGACGCGCG100.13856172925038104No Hit
CTTCCACCTCTCAGCGGAATTCCGACCATGTCAAGGGTAGGTAAGGTTTT100.13856172925038104No Hit
TTGCGGGACTTAACCCAACATCTCACGACACGAGCTGACGACAGCCATGC100.13856172925038104No Hit
CTTCCGGCCGGCGTGACCGGTGAGCAGCTGCATGCGTGTCTCGCGCACCA100.13856172925038104No Hit
CTACGCCGGCGCGCGTCACGTCGACGTCACGCCGCTGGCTGACGCGCACG100.13856172925038104No Hit
ACTAAGGACAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATCTCACG100.13856172925038104No Hit
AGCCAGCGGCGTGACGTCGACGTGACGCGCGCCGGCGTAGTGGTGCGCGA100.13856172925038104No Hit
CAGCAACACCTGTCCGCGTTCTTCATTGACGAACACGCCGAGCCGCAGGT100.13856172925038104No Hit
CCCCTGAGTCTGTCTTTAATTTACAGCCGATAAGCGTGAGCGCTCAACTT90.12470555632534293No Hit
GGCCGGCCTGCTGCCGCGCGACTACCCGGTGAGCATCCACGCGGTCTCCG90.12470555632534293No Hit
TCCACGCGGTCTCCGGTTACTCGGGAGGCGGAAGGGCCGCCGTCGAAGCA90.12470555632534293No Hit
GCTGCCGCGCGACTACCCGGTGAGCATCCACGCGGTCTCCGGTTACTCGG90.12470555632534293No Hit
CTCCCCAGGCGGTCAACTTCACGCGTTAGCTACGTTACTAAGGAAATGAA90.12470555632534293No Hit
GGGCGTGTGCAGGCACCGGCGCGACGCGGATCGGCCTGCCGGGCAGACCT90.12470555632534293No Hit
GGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCAACTAGTTGTT90.12470555632534293No Hit
ATCCACCGCTTGTGCGGGTCCCCGTCAATTCCTTTGAGTTTTAATCTTGC90.12470555632534293No Hit
GCCGCACCGGACGCCCCTTTGCCGAGATTGTCGAACACGGCGGACAGCAA90.12470555632534293No Hit
GAATGCCGCGGCGGGCGACGATAGGGCCTGAAATCGGCTAAAGGTGAGCT90.12470555632534293No Hit
TGCAGCACCTGTGCGCCGGTTCTCTTTCGAGCACTTCCACCTCTCAGCGG90.12470555632534293No Hit
ACGATAGGGCCTGAAATCGGCTAAAGGTGAGCTTTTACGGGGGGCGTGTG90.12470555632534293No Hit
GTCATGAATCCTACCGTGGTGACCGTCCTCCTTGCGGTTAGACTAGCCAC90.12470555632534293No Hit
TTGTATGACGTGTGAAGCCCTACCCATAAGGGCCATGAGGACTTGACGTC90.12470555632534293No Hit
GCAACACCTGTCCGCGTTCTTCATTGACGAACACGCCGAGCCGCAGGTCG90.12470555632534293No Hit
CCAACGACCTGCGGCTCGGCGTGTTCGTCAATGAAGAACGCGGACAGGTG90.12470555632534293No Hit
ACTACCCGGTGAGCATCCACGCGGTCTCCGGTTACTCGGGAGGCGGAAGG90.12470555632534293No Hit
GTGTTGCTGTCCGCCGTGTTCGACAATCTCGGCAAAGGGGCGTCCGGTGC90.12470555632534293No Hit
GGCTGACGCGCACGCAATCACGTATCTCGATCCGCAAGCGCTGAACGGCA90.12470555632534293No Hit
TTCCGATACGGCTACCTTGTTACGACTTCACCCCAGTCATGAATCCTACC90.12470555632534293No Hit
GGTCGTTGGTGCCGTTCAGCGCTTGCGGATCGAGATACGTGATTGCGTGC90.12470555632534293No Hit
GGCCTGAAATCGGCTAAAGGTGAGCTTTTACGGGGGGCGTGTGCAGGCAC90.12470555632534293No Hit
GGTGAGCACGATGCCTTGCCGATAGGCACCGTAGGCCGGCACGAAGAACG90.12470555632534293No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG90.12470555632534293No Hit
ACGCGGATCGGCCTGCCGGGCAGACCTTTGACGGTGCCTGCGCGAGATTC90.12470555632534293No Hit
ATCACGTATCTCGATCCGCAAGCGCTGAACGGCACCAACGACCTGCGGCT90.12470555632534293No Hit
ATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCAACTAGTTGTTGG90.12470555632534293No Hit
GTCTTTAATTTACAGCCGATAAGCGTGAGCGCTCAACTTTGCGAGAAGCT90.12470555632534293No Hit
GGTGTACGGCCTGGCGCTGGAGCACAAGCATGTTCCCGAGATCCGGCTGC90.12470555632534293No Hit
GTGTGTACAAGACCCGGGAACGTATTCACCGCGGCATGCTGATCCGCGAT90.12470555632534293No Hit
GGTGTTGCTGTCCGCCGTGTTCGACAATCTCGGCAAAGGGGCGTCCGGTG90.12470555632534293No Hit
ACCTTTGACGGTGCCTGCGCGAGATTCACGCTGCGTTCGACAGTCCGAGC80.11084938340030484No Hit
AGATCCGGCTGCATGCCGGGCTCGCACACCGCCCGTTCTTCGTGCCGGCC80.11084938340030484No Hit
GAGCAAAAGCAGGGATGAATGCCGCGGCGGGCGACGATAGGGCCTGAAAT80.11084938340030484No Hit
CTGCAACTCGAGTGCATGAAGCTGGAATCGCTAGTAATCGCGGATCAGCA80.11084938340030484No Hit
GTGTACAAGACCCGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTA80.11084938340030484No Hit
TGTCTGCACAGGCAGAAAACTGTGCAGTCAGGCACAGCGCGGGTTCTGTG80.11084938340030484No Hit
GTTCAGCGCTTGCGGATCGAGATACGTGATTGCGTGCGCGTCAGCCAGCG80.11084938340030484No Hit
TCCTCCTTGCGGTTAGACTAGCCACTTCTGGTAAAACCCACTCCCATGGT80.11084938340030484No Hit
GCGAAAGCAGGCCGGCCTGCTGCCGCGCGACTACCCGGTGAGCATCCACG80.11084938340030484No Hit
ATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGTCCTTAGTTGCTAC80.11084938340030484No Hit
GTAGTGGTGCGCGAGACACGCATGCAGCTGCTCACCGGTCACGCCGGCCG80.11084938340030484No Hit
GCATCAAGTCGAGATTCTGCACGGCCGCACCGGACGCCCCTTTGCCGAGA80.11084938340030484No Hit
TTGCCGAGATTGTCGAACACGGCGGACAGCAACACCTGTCCGCGTTCTTC80.11084938340030484No Hit
CTTTTACGGGGGGCGTGTGCAGGCACCGGCGCGACGCGGATCGGCCTGCC80.11084938340030484No Hit
GTGCGGCTGGATCACCTCCTTTCCAGAGCTTCTCGCAAAGTTGAGCGCTC80.11084938340030484No Hit
AACGTAGCTAACGCGTGAAGTTGACCGCCTGGGGAGTACGGTCGCAAGAT80.11084938340030484No Hit
GTCGAACGCAGCGTGAATCTCGCGCAGGCACCGTCAAAGGTCTGCCCGGC80.11084938340030484No Hit
CGTGTGCAGGCACCGGCGCGACGCGGATCGGCCTGCCGGGCAGACCTTTG80.11084938340030484No Hit
CCATGGGAGTGGGTTTTACCAGAAGTGGCTAGTCTAACCGCAAGGAGGAC80.11084938340030484No Hit
CGCCAAGGCGCTTACAACCACCAACACGTCAGACAATGGTGGGTCTGGTT80.11084938340030484No Hit
ATTAAAGACAGACTCAGGGGTCTGTAGCTCAGTCGGTTAGAGCACCGTCT80.11084938340030484No Hit
GTTCGACAGTCCGAGCATCAAGTCGAGATTCTGCACGGCCGCACCGGACG80.11084938340030484No Hit
CATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCT80.11084938340030484RNA PCR Primer, Index 27 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGCT100.0069463444145.0145
CGAAAGC203.710376E-4108.753
AGCGAAA203.710376E-4108.751
GAAAGCA259.010114E-487.04
GCGAAAG300.001858352772.52
AAAGCAG400.005810721354.3755
AAAAAAA5651.09048415E-86.159292135-139
Produced by FastQC (version 0.11.5)