FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l02_n01_Lib_prep_NC.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l02_n01_Lib_prep_NC.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAA53.546099290780142No Hit
GCAAAAGCAGGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAAGA21.4184397163120568No Hit
GCTTAGTGCTGGCCCATATCTCTTGTCCTCTTTGCCCAGAATGAGGAATC21.4184397163120568No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTTC21.4184397163120568No Hit
AGCAAAAGCAGGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAAG21.4184397163120568No Hit
TAGTGACCTTCCCCTTTTCGATCTTGAAAATTTTGTACGAGGCCAGCCCA10.7092198581560284No Hit
AAGCAGAGAGCACCATTCTCTCTATTGTTAAGCTGTTTTGGATCAACCGT10.7092198581560284No Hit
ATCAGGAAGACAGGAGAAGACTGAGGGGATTCCTCATTCTGGGCAAAGAG10.7092198581560284No Hit
GCTGTATATAGCCCACCCACGGATGGGACAAAGAGATGAATTGCCGGTTA10.7092198581560284No Hit
TCCATTAGGATCCCAAATCATCTCAAACCCATGTCTGGAACTGTGACTTT10.7092198581560284No Hit
CACTAGTCCAGATTGTTTTTTCTTTAGGTCGTCCCCTGATTAATTCAACC10.7092198581560284No Hit
TCGGTGACAACCCGCGTCCCGAAGATGGAACAGGCAGCTGTGGTCCAGTG10.7092198581560284No Hit
CTCATAGACTTTGGCACTCCTTCCGTAGAAGGCCCTCCTTTCAGTCCGTA10.7092198581560284No Hit
ATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACC10.7092198581560284No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT10.7092198581560284No Hit
ATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAAAACCACCGTG10.7092198581560284No Hit
CATCAGCTGTGTGATCAGTGCAGTGACATGTCACAATGTCCCTGTGGCCA10.7092198581560284No Hit
GCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTC10.7092198581560284No Hit
TAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAA10.7092198581560284No Hit
GGCCTATATCTCTTGTCCTCTTTGCCCAGAATGAGGAATCCCCTCAGTCT10.7092198581560284No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT10.7092198581560284No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCCCCGTTCCC10.7092198581560284No Hit
GTCTCGCACCCGCGAGATACTCACAAAAACCACCGTGGACCATATGGCCA10.7092198581560284No Hit
GAGGGAAGAGATCGCACAATGGCCTGGACAGTAGGCTCTTAGGGACAACC10.7092198581560284No Hit
TCCAGATGAGCTCATATTTTAAAAGGGCACAGAAAGGGTCTATATCCAAG10.7092198581560284No Hit
TCATATAGGTATGGTAATGGTGTTTGGATAGGAAGGACCAAAAGTCACAG10.7092198581560284No Hit
AATTAGCACATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGA10.7092198581560284No Hit
CTCTTAGGGATTTCTGATCTCGGCGAAGCCGATCAAGGAATGGGGCATCA10.7092198581560284No Hit
GGTATTAAAAGCATGAGCCATTGGGCCGGGTCAGGTCTTCTTTAATTTCT10.7092198581560284No Hit
GGATAGAGGCACCCTCTAAAAAGACAAGTTACTCACATTTGATCTGATGG10.7092198581560284No Hit
AACCTACCTGGGTTTTAGAAAAAATAAATCTGAGGTTGGGTTTGTTTCGG10.7092198581560284No Hit
CTGCTCCATTATCTGGACCTGAAATTCCGATTGTTAGCCAGCCCATGCCA10.7092198581560284No Hit
GAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATA10.7092198581560284No Hit
GAGAGGGGGAGGGGAATGTTTGGAGGCAGAGAGAGAGAGGAAGGGAAGTT10.7092198581560284No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCC10.7092198581560284No Hit
TTCTCTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCACAGAGACT10.7092198581560284No Hit
ACATGCACTTGCTGACCAAGCAACCGATTCAAATCTTGAATTGTACGGGG10.7092198581560284No Hit
ATCTGGAAAAGGCCAATAAAATTAAATCTGAGAAAACACACATCCACACT10.7092198581560284No Hit
GTCTAAGTGGTCGTGGTGTTGTTTTCAAAAAAGGTTCAATTCTAGCATTT10.7092198581560284No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA10.7092198581560284No Hit
GTTAAAGAATTTACCATACCGGATAATGCCCGAATATTTGATGGTTACGT10.7092198581560284No Hit
CCTACGAGCGTGTCGTAACCGTCGGACAACCGCATGATCAGGTCTGTCTC10.7092198581560284No Hit
CACTTGAGCCCAGGAAGTTGAGGCTGCAGCAAGCTGTAATCATGCCACAG10.7092198581560284No Hit
CAAATAGGGAATATAATCTCAATATGGATTAGCCATTCAATTCAAACTGG10.7092198581560284No Hit
CCTAAGAGCCTGAACGATAAGAAGCAGTTTTCTTGATTCAGCTGAAAATC10.7092198581560284No Hit
CCCTATGGCCATGGATTTGAGGCAGTTAACACACAGCCAATCAAAACCCT10.7092198581560284No Hit
CTACCATTGACCTCAAAGCGGCTGAAATCTCCACTTGCAAATTCCAGAAA10.7092198581560284No Hit
ATATTAACCGGCAATTCATCTCTTTGTCCCATCCGTGGGTGGGCTATATA10.7092198581560284No Hit
CTTCCGCTATACCCTGACCAATCAGTCATTGCCACAACATCTTGCCTAAC10.7092198581560284No Hit
GTGATGTGTGTGTGCAGAGACAACTGGCATGGTTCGAACCGGCCATGGGT10.7092198581560284No Hit
GAACCCAATGTTGTTAAACCACACGAAAAGGGAATAAATCCAAATTATCT10.7092198581560284No Hit
CTCAAAAAAACCACCGTGGACCATATGGCCATAATCAAGAAGTACACGTC10.7092198581560284No Hit
TCTGACTAAGGGGATTTTAGGATTTGTGTTCACGCTCACCGTGCCCAGTG10.7092198581560284No Hit
CCTTTCGCAAGGTTGCTCAGTTCATTGATGCTTAGTGCTGGCCCATATCT10.7092198581560284No Hit
ATGACTGATTGGTCAGGGTATAGCGGAAGTTTCGTTCAACATCCTGAGCT10.7092198581560284No Hit
GTGGAGTCTGCCAAATTCTGCCTCTTACACTTTCAGCTACCACGCCATGT10.7092198581560284No Hit
GACCTAGCCTGCCCTGGGTTCTGATTCTGGGGTAAACAGAACAGTCCTTT10.7092198581560284No Hit
GGACACAAGAGTCTGAATGTGCCTGTGTAAATGGTTCATGTTTTACTATA10.7092198581560284No Hit
GACCCACACCATCACCTCGAAGCGCCGTCTCAAGTCCCTAGTAACCGACA10.7092198581560284No Hit
TTCTGACCCAAGGTGCCTTACTGAATGACAAGCATTCAAATGGGACTGTT10.7092198581560284No Hit
TCAGACTCTTGTGTCCTCAATATTTTCTTCCTCCAACTTTTTATGGTTTC10.7092198581560284No Hit
GACTGAAAGGAGGGCCTTCTACGGAAGGAGTGCCAAAGTCTATGAGGGAA10.7092198581560284No Hit
GAGTTGAACCAAGAAGCATTAAGCAAAACCCAGGGATCATTAATCAGGCA10.7092198581560284No Hit
TTTTTGTTGAATCTGCAAGTGGACATTTGGATAGATTTGAAGATTTCGTT10.7092198581560284No Hit
GAAATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAAAACCACC10.7092198581560284No Hit
ATCTTGAATTGTACGGGGACGGAGCTTCACCGACAGGGCAGCTCATTAAG10.7092198581560284No Hit
TCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCG10.7092198581560284No Hit
CAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTA10.7092198581560284No Hit
TGTTTCTACTAATAACCCGAGTAGAAACAAGGGTGTTTTTTATTATTAAA10.7092198581560284No Hit
CCTTGTGATCTGCCCACCTCGGCCTCCCAAAGTGCTGCGATTATAGGTGT10.7092198581560284No Hit
TCATTCAGTAAGGCACCTTGGGTCAGAAAAAAGGTCCTGCATTCCAAGTG10.7092198581560284No Hit
GGTCACACACATGATTATTAGGCTTCAGGTTTTTTACTGGCTGCTGAACC10.7092198581560284No Hit
TTCCCCTGCACTCGGGTTGATTCCATTCCATTCCATTACATTACATTCCA10.7092198581560284No Hit
GGTCAGGGTATAGCGGAAGTTTCGTTCAACATCCTGAGCTAACAGGGCTA10.7092198581560284No Hit
TCCCTCTACTCCGTTTGCTCCATCAACATACACTGGACCACAGCTGCCTG10.7092198581560284No Hit
GCCTCCAGCTCGCGGTCGGCGCGAACTCGCCGTACCTCCTCGGCAAGGAG10.7092198581560284No Hit
GATCTTCATGATCTCAGTGAACTCTTCTTTCTTTATCCTTCCAGATTCGA10.7092198581560284No Hit
AAAGCTTCCCTAGGCAAGTTTTTCACCTTAGTAATTTTCCACCCCAACCC10.7092198581560284No Hit
ACCTATAAAAATAGCACCTGGGTAAAGGACACAACTTCAGTGATATTAAC10.7092198581560284No Hit
AAAGCAGGGAGACAGGGAGGGAATCTGGGACTGAGAGGGGGAGGGGAATG10.7092198581560284No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT10.7092198581560284No Hit
CCATACTGGAATATGCAACCAAAACATCATTACCTATAAAAATAGCACCT10.7092198581560284No Hit
TATGTATGCATCTCCACAACTAGAAGGATTTTCAGCTGAATCAAGAAAAC10.7092198581560284No Hit
GGCGTGCACGCCTGCGCGTTCATCCTGTCGAGTACCCCACTGCTTGACCT10.7092198581560284No Hit
GTCTCTGTCCATCCATTAGGATCCCAAATCATCTCAAACCCATGTCTGGA10.7092198581560284No Hit
TAAATGACCTGGTTCTGAGTAACTGGTTGGACAGCTGTACCCAAGAGGTT10.7092198581560284No Hit
GGACGAAGGTGCTCCCAAGAGGGAAGCTTTCCACTAGAGGAGTTCAAATT10.7092198581560284No Hit
ACCTTGTTTCTACTAATAACCCGAGAAACAAGGTCGTTTTTAAACTATTC10.7092198581560284No Hit
GCACCAATGTATGCTTTCGCACTGAGTAGAGGCTTTGGGTCCGGCATCAT10.7092198581560284No Hit
GGTTTAAAATGAATCCAAATCAGAAAATAACAACCATTGGATCAATCTGT10.7092198581560284No Hit
GAAGGAGTTGAACCAAGAAGCATTAAGCAAAACCCAGGGATCATTAATCA10.7092198581560284No Hit
AGTCCAGATTGTTTTTTCTTTAGGTCGTCCCCTGATTAATTCAACCCAGA10.7092198581560284No Hit
TCATTATAGTAAAACATGAACCATTTACACAGGCACATTCAGACTCTTGT10.7092198581560284No Hit
CTTCTACACTCCCACAAAGTTCAGAGTTTAGCAGGGATGAGGAGCGTACT10.7092198581560284No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC10.7092198581560284No Hit
TCACTATTCACGCCACAAAAAGAAATGCTGCTCGCACTAGTCCAGATTGT10.7092198581560284No Hit
GGACCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATG10.7092198581560284No Hit
AGAGAGGAGAAGAGACAATTGAAGAAAGGTTTGAAATCACAGGAACAATG10.7092198581560284No Hit
GAGTTCTCTCATCCACTTTCCGTTTACTCTCCTGTATATAGGTCCTCCAG10.7092198581560284No Hit
AGACTTTGGCACTCCTTCCGTAGAAGGCCCTCCTTTCAGTCCGTATTTAA10.7092198581560284No Hit
TCTCTTGTCCTCTTTGCCCAGAATGAGGAATCCCCTCAGTCTTCTCCTGT10.7092198581560284No Hit
ATCCTTCTAGTTGTGGAGATGCATACAAGCTGTTGAATACCGACTTTGCT10.7092198581560284No Hit
AAGCCTTCTTTTCTAACTGCAGAGGGATAACTGCTGTCTCTTATACACAT10.7092198581560284No Hit
GTCAGTATATAGCGGAAGTTTCGTTCAACATCCTGAGCTAACAGGGCTAG10.7092198581560284No Hit
GTGTGGAACTTTGCAAACTCGGGTCCTCACCCGAGGACCGCACGGCCGAC10.7092198581560284No Hit
GTTAAGGACAGAAGCCCTTATAGGGCCTTAATGAGCTGCCCTGTCGGTGA10.7092198581560284No Hit
CTTCCCTTCTGACCCTTTGCAGATGGTAAAGGAAGGAACCTGTCTCTTAT10.7092198581560284No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG10.7092198581560284No Hit
GGAAGACAGGAGAAGACTGAGGGGATTCCTCACTCTGGGCAAAGAGGACA10.7092198581560284No Hit
CATAAGCAATTCTTGTTTTTCGTCCATTCTCACCTCTCCAGAAGTTCCGA10.7092198581560284No Hit
TCCATACCCAGTAAATGAAATCCCTTCCCTACCACACCTGTCTCTTATAC10.7092198581560284No Hit
GAGTAGAGGCTTTGGGTCCGGCATCATCACCTCAAACGCATCAATGCATG10.7092198581560284No Hit
ATTTTAATACAGCCACTGCTCCATTATCTGGACCTGAAATTCCGATTGTT10.7092198581560284No Hit
CATGAAATAAAGGGCTCTCTTATGACAAAAACGTCTCCTTTGGAACCAAT10.7092198581560284No Hit
AAAACGTCTCCTTTGGAACCAATTCTTATGCTATTGTCTTTGCTGTATAT10.7092198581560284No Hit
GTATGCATCTCCACGACTAGAAGGATTTTCAGCTGAATCAAGAAAACTGC10.7092198581560284No Hit
AATCTACAGTATCACTATTCACGCCACAAAAAGAAATGCTGCTCGCACTA10.7092198581560284No Hit
TTATCAAATAGGATACATCTGCAGTGGGGTTTTCGGTGACAACCCGCGTC10.7092198581560284No Hit
AGCCCTTCCTGACCTCCCTGACCAGGCCCATTTCCAATATTTTGTACTCA10.7092198581560284No Hit
TCCTAGTGGAGTTTAATAAGACCTGAACATTTTCTGTACCAATCCTGTCT10.7092198581560284No Hit
CCGTCGACCGGGCCACCGCGAGCAGTCCGTAGCTGATGGCGAACCAGACC10.7092198581560284No Hit
GGATGGGATTCCTCAAGGAAAGCCATTGCTTCCAATACACAATCTGTTTG10.7092198581560284No Hit
CTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGGCCATCAATTA10.7092198581560284No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA10.7092198581560284No Hit
CTTAAAAAGAATTTTGAAAATCTAATCAAGGTTAAAAGGCACCTTACAGA10.7092198581560284No Hit
TTCTCGACGTGGCACGCTACACCAAAGGCAAACGCTGTCTCTTATACACA10.7092198581560284No Hit
CTACTTGTCAATGCTGAATGGCAACTCAGCACCGTCTGGCCAAGACCAAT10.7092198581560284No Hit
GACCTTAAAAAGATTTGTGTATCTTTTTAAGGTCCCACTCATTAGGGTTT10.7092198581560284No Hit
GACCATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAAGAC10.7092198581560284No Hit
CAGTATCACTATTCACGCCACAAAAAGAAATGCTGCTCGCACTAGTCCAG10.7092198581560284No Hit
GTTCATTGATGCTTAGTGCTGGCCCATATCTCTTGTCCTCTTTGCCCAGA10.7092198581560284No Hit
TTCTTTACCCACAGCTGTGAATTGAATGTTCATTTTCTCGATAACAGTGT10.7092198581560284No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT10.7092198581560284No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers