FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l02_n01_KO_PR8_M2_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l02_n01_KO_PR8_M2_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4210919
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT567541.3477818024996444No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT436151.0357596524654118No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC398890.9472754047275666No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA388350.9222452391033882No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA294450.6992535358671113No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA236370.5613263992966856No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTT227670.5406658261533884No Hit
ATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA225880.536414972598618No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG213670.5074189268423354No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGTGAACTCTTCTTTCTTT127290.30228555809313834No Hit
CCCTTATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATACA120040.28506841380705733No Hit
TAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAA120010.2849971704513908No Hit
GGATACACCATGGATACTGTCAACAGGACACATCAGTACTCAGAAAAGGG119590.2839997634720592No Hit
TTCTTTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGGGCTCT116690.27711290575762676No Hit
AGCGAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAG111760.2654052476430917No Hit
GAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATA110880.2633154425435398No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTTGC109850.26086942066565516No Hit
GCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTC107660.2556686557019976No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT106600.2531513904684464No Hit
GCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAGA100850.23949641396569252No Hit
GAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT100740.23923518832824853No Hit
ATATGAAGCAATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTA98770.23455687463947894No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT97930.23256206068081575No Hit
GGGTCTTCAGTTATTTCTCTCAGGAATCATTTCCGTTATCCTCTTGTCTG96260.22859618054871159No Hit
GGACAAGCTAAATTCACTATTTTTGCCGTCTGAGCTCTTCAATGGTGGAA93930.22306294659194345No Hit
GTATGGTGGAGGCTATGGTTTCCAGAGCCCGAATTGATGCACGGATTGAT92450.21954827437906071No Hit
GAGCGAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA90930.21593861102528925No Hit
CTTAGGGACAACCTTGAACCTGGGACCTTTGATCTTGGGGGGCTATATGA90310.21446624834151407No Hit
GCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTTTC88610.21042912485374332No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCC85910.2040172228437545No Hit
ATATACTCACTTGGAAGTATGCTTCATGTATTCAGATTTTCACTTCATCA85420.20285358136786766No Hit
ATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATACACCATG82500.1959192280829909No Hit
CTTCTTTCTTTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGG78230.18577892379311975No Hit
AAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCCGAAT77710.18454403896156635No Hit
GAGCGAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT77390.18378410983445656No Hit
GTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAGCAAAACCCAGGGATCA77350.18368911869356783No Hit
ATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTAATGCTTCTTG76400.18143307909746068No Hit
AGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAG76120.18076814111123962No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAC75030.17817963252202193No Hit
GCTAAATTCACTATTTTTGCCGTCTGAGCTCTTCAATGGTGGAACAGATC74260.176351053059914No Hit
GCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCA73200.17383378782636286No Hit
CCATAGCCTCCACCATACTGGATATCCCGACTGGTCTTCTGTATGAACTG72130.1712927748075895No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTTTGCCG72040.17107904474058988No Hit
ATACTGGATATCCCGACTGGTCTTCTGTATGAACTGCTGGGGAAGAATTT70770.16806307601737294No Hit
AGCGAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTT70760.16803932823215073No Hit
ATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAAAACCACCGTG70750.16801558044692858No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT70400.16718440796415224No Hit
GAAGCAGTTTTCTTGATTCAGCTGAAAATCCTTCTAGTTGTGGAGATGCA69850.16587827977693229No Hit
CCTCCATACTGGATATCCCGACTGGTCTTCTGTATGAACTGCTGGGGAAG69620.16533208071682215No Hit
GGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATG67910.16127120944382922No Hit
AACTAACTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAG67470.1602263068940533No Hit
GAGTGCATATTGCTGCAAATTTGTTTGTTTCGATTTTCAGGTCCTCCCCA66770.15856396192850064No Hit
GTTAAGGACAGAAGCCCTTATAGGGCCTTAATGAGCTGCCCTGTCGGTGA66310.15747156380828034No Hit
CCTTACAGCCATGGGACAGGAACAGGATACACCATGGATACTGTCAACAG65930.15656914796983748No Hit
ACCTTGAACCTGGGACCTTTGATCTTGGGGGGCTATATGAAGCAATTGAG65680.15597545333928295No Hit
CATCAGTACTCAGAAAAGGGAAGATGGACAACAAACACCGAAACTGGAGC64700.15364817038750925No Hit
TCATTAATCAGGCACTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAA64000.15198582542195657No Hit
GCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAG63220.15013349817462648No Hit
TCAATGAACTGAGCAACCTTGCGAAAGGAGAGAAGGCTAATGTGCTAATT61920.147046286095743No Hit
ATCCAGTATGGTGGAGGCTATGGTTTCCAGAGCCCGAATTGATGCACGGA61860.14690379938440992No Hit
AGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGT60880.14457651643263622No Hit
GCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAAGGTTGTCCCT60130.14279543254097266No Hit
AGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTT59640.1416317910650858No Hit
ATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTC59180.14053939294486548No Hit
CATCATGGTTTCCAGAGCCCGAATTGATGCACGGATTGATTTCGAATCTG59030.14018317616653278No Hit
ATATACAGCAAAGACAATAGCATAAGAATTGGTTCCAAAGGAGACGTTTT58830.13970822046208917No Hit
GAGTATATTGCTGCAAATTTGTTTGTTTCGATTTTCAGGTCCTCCCCATA58410.13871081348275757No Hit
CTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAA58130.1380458754965365No Hit
CCACCATACTGGATATCCCGACTGGTCTTCTGTATGAACTGCTGGGGAAG57520.1365972605979835No Hit
GATGTGTACTTCTTGATTATGGCCATATGGTCCACGGTGGTTTTTGTGAG57420.1363597827457617No Hit
GTCCTTAACAGTCCCATTTGAATGCTTGTCATTCAGTAAGGCACCTTGGG57170.13576608811520716No Hit
GTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCACT57120.13564734918909627No Hit
GGATACACTGTCAACAGGACACATCAGTACTCAGAAAAGGGAAGATGGAC56600.13441246435754287No Hit
ATATGCACTCACTTGGAAGTATGCTTCATGTATTCAGATTTTCACTTCAT55800.13251264153976838No Hit
GTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCGCTATACCCTG55500.131800207983103No Hit
CCTCCACCATACTGGATATCCCGACTGGTCTTCTGTATGAACTGCTGGGG55100.13085029657421574No Hit
GGCCAGCACTAAGCATCAATGAACTGAGCAACCTTGCGAAAGGAGAGAAG54170.12864175254855295No Hit
TCTTTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGGGCTCTG53720.1275731022135548No Hit
GATCTTCATGATCTCAGTGAACTCTTCTTTCTTTATCCTTCCAGATTCGA53340.12667068637511195No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGT53180.12629072181155704No Hit
AGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTTT52850.12550704489922507No Hit
GTATATAGCCCACCCACGGATGGGACAAAGAGATGAATTGCCGGTTAATA52570.12484210691300403No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG52520.12472336798689312No Hit
GTACACATCAGGAAGACAGGAGAAGAACCCAGCACTTAGGATGAAATGGA51900.1232510053031179No Hit
AGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTTT51880.12320350973267355No Hit
ATGCTATAAGCACAACTTTCCCTTATACTGGAGACCCTCCTTACAGCCAT51370.12199237268634233No Hit
CAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCATTAA49950.11862018718479267No Hit
GTATGAACTGCTGGGGAAGAATTTTTCAAATAAATTGCAGCACCTTTGGT49590.11776526691679418No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA48620.11546173175024264No Hit
TTCATGATCTCAGTGAACTCTTCTTTCTTTATCCTTCCAGATTCGAAATC48290.11467805483791067No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTTT47770.11344317000635729No Hit
AGTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATC47720.1133244310802464No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA47300.1123270241009148No Hit
CCATTTGAATGGATGTCAATCCGACCTTACTTTTCTTAAAAGTGCCAGCA46150.10959602880036401No Hit
GTGTATCCTGTTCCTGTCCCATGGCTGTAAGGAGGGTCTCCAGTATAAGG45730.10859862182103242No Hit
GGATAGCAGTTCATACAGAAGACCAGTCGGGATATCCAGTATGGTGGAGG45720.10857487403581025No Hit
ATCAGGCACTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGTCCC45120.10715000692247939No Hit
GGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGA45030.10693627685547977No Hit
ATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAAGGTTGTCCCTAAGAG44510.10570139202392638No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTTC44490.10565389645348201No Hit
TGTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAGCAAAACCCAGGGATC44280.10515519296381622No Hit
ATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACC43970.10441901162192861No Hit
TCTTAAAAGTGCCAGCACAAAATGCTATAAGCACAACTTTCCCTTATACT43960.10439526383670641No Hit
GTCTCGCACCCGCGAGATACTCACAAAAACCACCGTGGACCATATGGCCA43450.10318412679037521No Hit
TTTCTTAAAAGTGCCAGCACAAAATGCTATAAGCACAACTTTCCCTTATA43110.10237670209282107No Hit
GTATTAAAATACAACGGCATAATAACTGAAACCATAAAAAGTTGGAGGAA42900.10187799860315527No Hit
GGTATGGTAATGGTGTTTGGATAGGAAGGACCAAAAGTCACAGTTCCAGA42580.10111806947604549No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC100.007092323144.9955145
GCTTAGA100.007092323144.9955145
GAGCGAA32950.0140.43171
GCAAAAG226450.0103.517713
GAGCAAA227150.0103.386541
AGCAGGC96950.0101.324868
AGTAGAA57450.0101.121231
CAAAAGC236900.099.043214
CGGCATG301.5992675E-596.67635
GCAGGCA103850.094.453029
ATTAGTA6300.094.400261
AAAAGCA248900.094.384665
AAGCAGG308500.091.015817
TGACGGC259.384699E-487.0097052
TATATAT259.385142E-487.008673
AAAGCAG334300.084.0666056
GCAGGGG25550.083.138329
GCAGGTC86200.081.412799
AGCAGGG38550.078.609758
GCAGGGT11600.078.1225749
Produced by FastQC (version 0.11.5)