FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l02_n01_KO_PR8_M1_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l02_n01_KO_PR8_M1_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3012314
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT275470.9144796989955231No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC239920.7964641136349No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT186310.6184946190868549No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA177230.5883516791410192No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA145600.4833493453869683No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT128880.42784384363648675No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA97280.32294110109371066No Hit
TAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAA80070.26580894289240764No Hit
GGTTCAAGGTTGTCCCTAAGAGCCTGAACGATAAGAAGCAGTTTTCTTGA80010.26560976046985807No Hit
GAAGCAGTTTTCTTGATTCAGCTGAAAATCCTTCTAGTTGTGGAGATGCA75420.25037230514481557No Hit
GTTAAGGACAGAAGCCCTTATAGGGCCTTAATGAGCTGCCCTGTCGGTGA74460.24718538638402238No Hit
GTCCTTAACAGTCCCATTTGAATGCTTGTCATTCAGTAAGGCACCTTGGG67830.22517572869229438No Hit
GTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCGCTATACCCTG66570.2209928978187533No Hit
GCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTC65700.21810475269178448No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTT65620.21783917612838502No Hit
ATATACAGCAAAGACAATAGCATAAGAATTGGTTCCAAAGGAGACGTTTT65180.21637850502968814No Hit
TTTGCAAGGTCTGCAGGACTTTATTAGCAAAGTCGGTATTCAACAGCTTG59600.1978545397325777No Hit
GTATATAGCCCACCCACGGATGGGACAAAGAGATGAATTGCCGGTTAATA55570.18447612035133124No Hit
ATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA53490.17757112970294597No Hit
GGTTCGTCCCTAAGAGCCTGAACGATAAGAAGCAGTTTTCTTGATTCAGC53250.17677440001274766No Hit
CCCTTATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATACA52850.1754465171957505No Hit
GTTCAAGGTTGTCCCTAAGAGCCTGAACGATAAGAAGCAGTTTTCTTGAT52810.17531372891405078No Hit
GAGCGAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT50270.16688167302611878No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA49090.16296441871597717No Hit
GGTATGGTAATGGTGTTTGGATAGGAAGGACCAAAAGTCACAGTTCCAGA46500.1543663774759205No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG46430.154133997982946No Hit
GTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAGCAAAACCCAGGGATCA46260.1535696477857222No Hit
ATATGAAGCAATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTA45920.15244094739127462No Hit
TCATTAATCAGGCACTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAA44480.1476605692500848No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG43510.1444404534188667No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAC42360.14062279031999983No Hit
GCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCA41700.13843178367195452No Hit
GTATTAAAATACAACGGCATAATAACTGAAACCATAAAAAGTTGGAGGAA41520.13783423640430578No Hit
AGCGAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTT41460.1376350539817562No Hit
GTACATGACCAAAGAGTTCTTTGAGAACAAATCAGAAACATGGCCCATTG41300.13710390085495736No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGTGAACTCTTCTTTCTTT40600.13478010592521233No Hit
AACTAACTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAG40390.1340829674462888No Hit
TACCATACCTATATGAAAATCCCTTTACTCCGTTTGCTCCATCAACATAC39960.13265549341801686No Hit
GTATGGGGAGGACCTGAAAATCGAAACAAACAAATTTGCAGCAATATGCA39730.13189196079824347No Hit
GCCTCATACAGTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCG39690.13175917251654376No Hit
CTTAGGGACAACCTTGAACCTGGGACCTTTGATCTTGGGGGGCTATATGA39590.13142720181229448No Hit
AGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGT38560.1280079035585268No Hit
GCTGTATATAGCCCACCCACGGATGGGACAAAGAGATGAATTGCCGGTTA37730.12525254671325764No Hit
GCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCATTG37470.12438942288220949No Hit
CAATATGGATTAGCCATTCAATTCAAACTGGAAGTCAAAACCATACTGGA37450.12432302874135963No Hit
GGATTAGCCATTCAATTCAAACTGGAAGTCAAAACCATACTGGAATATGC37000.12282916057223782No Hit
GCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAAGGTTGTCCCT36700.12183324845948995No Hit
GCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAG36470.12106971583971658No Hit
AGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTT36320.12057175978334264No Hit
CTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAA35940.11931027110719535No Hit
TTTTTGAACAGACTACTTGTCAATGCTGAATGGCAACTCAGCACCGTCTG34690.11516063730407919No Hit
GTGTAAATGGTTCATGTTTTACTATAATGACTGATGGCCCGAGTGATGGG34550.11469587831813018No Hit
ATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATACACCATG34350.11403193690963159No Hit
GGACAACCTTGAACCTGGGACCTTTGATCTTGGGGGGCTATATGAAGCAA34320.11393234569835682No Hit
GGATACACCATGGATACTGTCAACAGGACACATCAGTACTCAGAAAAGGG34210.1135671779236826No Hit
ATACTGGATATCCCGACTGGTCTTCTGTATGAACTGCTGGGGAAGAATTT34200.11353398085325765No Hit
GACTACTTGTCAATGCTGAATGGCAACTCAGCACCGTCTGGCCAAGACCA34140.11333479843070808No Hit
TTCTTTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGGGCTCT34080.11313561600815852No Hit
ATATAGCCCACCCACGGATGGGACAAAGAGATGAATTGCCGGTTAATATC34040.1130028277264588No Hit
GATCTTCATGATCTCAGTGAACTCTTCTTTCTTTATCCTTCCAGATTCGA33870.112438477529235No Hit
GAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT33100.10988230310651546No Hit
ATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTAATGCTTCTTG32880.10915196755716702No Hit
CCTTACAGCCATGGGACAGGAACAGGATACACCATGGATACTGTCAACAG32870.1091187704867421No Hit
ATCAGGCACTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGTCCC31650.10506872789490074No Hit
ACTCAGCACCGTCTGGCCAAGACCAATCTACAGTATCACTATTCACGCCA31320.10397322457087806No Hit
ACATACACTGGACCACAGCTGCCTGTTCCATCTTCGGGACGCGGGTTGTC31060.10311010073982992No Hit
GTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCACT30660.10178221792283275No Hit
CAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCATTAA30450.10108507944390924No Hit
TTACCATACCTATATGAAAATCCCTTTACTCCGTTTGCTCCATCAACATA30190.10022195561286108No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGTG100.007087823145.02562
CCGGTAC100.007087823145.02563
GAGCGAA16950.0138.208271
AGCAGGC34300.0102.512268
GCAAAAG116800.0102.436753
CAAAAGC130000.092.147034
GACGTGC259.374589E-487.03271
AAGCGGG259.389439E-486.998047
AAGCAGG166350.085.725447
ATTAGTA3450.084.089571
GCAGGGT13750.081.725429
GAGCAAA149750.080.397491
GCAGGGG31900.079.9981849
GCAGGCA44600.078.83799
TATTAGT1300.078.106271
AAAAGCA153350.077.974315
AGCAGGG48600.075.6318
AAAGCAG192850.074.108376
AGCGAAA31600.073.8896262
GGGCCGG300.001933237972.5272451
Produced by FastQC (version 0.11.5)