FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l02_n01_KO_PR8_F1_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l02_n01_KO_PR8_F1_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2623195
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT312741.192210262675859No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT203590.7761146235792612No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC203440.7755428018122938No Hit
TTTCTTAAAAGTGCCAGCACAAAATGCTATAAGCACAACTTTCCCTTATA203190.7745897655340148No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTT170370.6494751629215517No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA143100.5455179656868818No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA121620.4636330886571528No Hit
GGATACACCATGGATACTGTCAACAGGACACATCAGTACTCAGAAAAGGG102820.3919647605305744No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA88100.3358499844655087No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGTGAACTCTTCTTTCTTT82520.31457821473432207No Hit
CCTCTTTGACTTGTATTCAAGATGGATCGATTTACTTTGCCGGTATCAGG79830.30432354438004033No Hit
CCCTTATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATACA75800.2889605995741834No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT73290.27939211534026254No Hit
CCTTACAGCCATGGGACAGGAACAGGATACACCATGGATACTGTCAACAG71510.2726064970389163No Hit
ATACTGGATATCCCGACTGGTCTTCTGTATGAACTGCTGGGGAAGAATTT70250.26780319419639026No Hit
TAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAA62880.23970768471272627No Hit
GGACAAGCTAAATTCACTATTTTTGCCGTCTGAGCTCTTCAATGGTGGAA62400.23787785505843068No Hit
ATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA59460.22667014842587No Hit
GAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT57090.21763536450778534No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG57050.21748287870326072No Hit
CTTAGGGACAACCTTGAACCTGGGACCTTTGATCTTGGGGGGCTATATGA54780.2088293092964877No Hit
ATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATACACCATG54400.20738069415350366No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTTTGCCG51940.19800281717523857No Hit
GCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTC51940.19800281717523857No Hit
GCTAAATTCACTATTTTTGCCGTCTGAGCTCTTCAATGGTGGAACAGATC50310.19178902064085973No Hit
GCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTTTC49990.19056913420466265No Hit
ATATGAAGCAATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTA48510.18492715943725116No Hit
GTTAAGGACAGAAGCCCTTATAGGGCCTTAATGAGCTGCCCTGTCGGTGA48420.1845840663770707No Hit
GTCCTTAACAGTCCCATTTGAATGCTTGTCATTCAGTAAGGCACCTTGGG45060.17177525879700137No Hit
CTTTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGGGCTCTGG44590.1699835505938369No Hit
ACCTTGAACCTGGGACCTTTGATCTTGGGGGGCTATATGAAGCAATTGAG43100.16430345437529426No Hit
ATATACAGCAAAGACAATAGCATAAGAATTGGTTCCAAAGGAGACGTTTT42470.16190180295403125No Hit
GTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCGCTATACCCTG42330.16136810263819504No Hit
CCATAGCCTCCACCATACTGGATATCCCGACTGGTCTTCTGTATGAACTG41900.1597288802395552No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAC41820.15942390863050593No Hit
TTCTTTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGGGCTCT41530.15831838654770233No Hit
GTATATAGCCCACCCACGGATGGGACAAAGAGATGAATTGCCGGTTAATA40150.1530576262916024No Hit
CCACCATACTGGATATCCCGACTGGTCTTCTGTATGAACTGCTGGGGAAG39890.15206646856219228No Hit
GTATGAACTGCTGGGGAAGAATTTTTCAAATAAATTGCAGCACCTTTGGT38670.14741565152419092No Hit
GCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAAGGTTGTCCCT38500.14676758685496122No Hit
GAGCGAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT38280.14592891493007573No Hit
GCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCA37920.144556542689354No Hit
TCATTAATCAGGCACTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAA37870.14436593543369822No Hit
AGCGAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTT36990.14101124773415627No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTTT36720.13998196855361497No Hit
GTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAGCAAAACCCAGGGATCA35790.13643667359841719No Hit
AACTAACTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAG35760.13632230924502373No Hit
GAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATA34950.13323447170339986No Hit
CTTTAATTCGGGCTCTGGAAACCATAGCCTCCACCATACTGGATATCCCG34920.13312010735000637No Hit
GCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAG34460.13136652059797307No Hit
GGATACCAAAGGTGCTGCAATTTATTTGAAAAATTCTTCCCCAGCAGTTC34240.1305278486730876No Hit
AGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTTT34140.130146634161776No Hit
ATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTAATGCTTCTTG33700.12846929031200502No Hit
CTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAA33520.12778310419164415No Hit
GTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCACT33040.12595327453734853No Hit
CGTTCCTGTTACCCTGATACCGGCAAAGTAAATCGATCCATCTTGAATAC32380.12343725876269206No Hit
GTACTGATGTGTCCTGTTGACAGTATCCATGGTGTATCCTGTTCCTGTCC32250.122941679897987No Hit
GGTATGGTAATGGTGTTTGGATAGGAAGGACCAAAAGTCACAGTTCCAGA31110.11859583446903489No Hit
GTATTAAAATACAACGGCATAATAACTGAAACCATAAAAAGTTGGAGGAA30950.11798589125093636No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTTGC30710.11707097642378855No Hit
GTATTCAAGATGGATCGATTTACTTTGCCGGTATCAGGGTAACAGGAACG30490.11623230449890305No Hit
AGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGT30370.11577484708532916No Hit
GAAGCAGTTTTCTTGATTCAGCTGAAAATCCTTCTAGTTGTGGAGATGCA30370.11577484708532916No Hit
CCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAAGG30030.11447871774686975No Hit
AGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTTT29400.11207706632560675No Hit
AGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTT28820.10986602215999955No Hit
ATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAAGGTTGTCCCTAAGAG28430.10837928556588436No Hit
ATTCAAGATGGATCGATTTACTTTGCCGGTATCAGGGTAACAGGAACGTT28210.10754061364099887No Hit
AGCGAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAG28140.10727376348308075No Hit
GGCTAATGTGCTAATTGGGCAAGGAGACGTGGTGTTGGTAATGAAACGGA28010.10677818461837568No Hit
TTTCTTGCCAGCACAAAATGCTATAAGCACAACTTTCCCTTATACTGGAG27880.10628260575367061No Hit
GGTGTATCCTGTTCCTGTCCCATGGCTGTAAGGAGGGTCTCCAGTATAAG27870.10624448430253945No Hit
CAATATGGATTAGCCATTCAATTCAAACTGGAAGTCAAAACCATACTGGA27780.10590139124235903No Hit
GTCATCGTTCAGGCTCTTAGGGACAACCTTGAACCTGGGACCTTTGATCT27570.1051008407686047No Hit
CCATGGATACTGTCAACAGGACACATCAGTACTCAGAAAAGGGAAGATGG27240.10384283288127646No Hit
GGATTAGCCATTCAATTCAAACTGGAAGTCAAAACCATACTGGAATATGC27170.10357598272335836No Hit
ATCAGGCACTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGTCCC27150.10349973982109603No Hit
GCTGTATATAGCCCACCCACGGATGGGACAAAGAGATGAATTGCCGGTTA27130.10342349691883372No Hit
CAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCATTAA27060.10315664676091561No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG27050.10311852530978444No Hit
TACCATACCTATATGAAAATCCCTTTACTCCGTTTGCTCCATCAACATAC27020.10300416095639098No Hit
GGATATCCCGACTGGTCTTCTGTATGAACTGCTGGGGAAGAATTTTTCAA26850.10235609628716127No Hit
TTTTTGAACAGACTACTTGTCAATGCTGAATGGCAACTCAGCACCGTCTG26280.10018317357268522No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTTC26240.10003068776816058No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGGCG100.0070878766145.024891
GAGCGAA15950.0136.841661
CGCATGT203.8658842E-4108.756226
GCAAAAG109800.0104.398053
AGCAGGC60600.099.416428
CAAAAGC116700.098.225424
AGTAGAA39550.095.888761
GAGCAAA124000.093.096621
GCAGGCA66850.090.230149
AAAAGCA129400.088.8652045
AAGCAGG154900.088.130987
ACGGTGT353.4373443E-582.861884
ATTAGTA4500.082.180761
AAAGCAG171550.079.710186
GCAGGGG17050.076.1129159
GCAGGGT7350.073.978189
AGCGAAA29800.072.990752
CTCAACG300.001934776772.5124361
CGTCCAG300.001935654872.504145
GCAGGTC24850.071.769269
Produced by FastQC (version 0.11.5)