FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l02_n01_KO_PBS_M1_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l02_n01_KO_PBS_M1_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1707307
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGAGACAGGGAGGGAATCTGGGACTGAGAGGGGGAGGG108210.6338051680219199No Hit
GAGTAGGGGCTGGTGGGAGAAGTGTGTGATGTGGACCATGGTAAAAGCTA46370.27159731670988285No Hit
GAGCAAAAGCAGGCCCAAGCAGCCAAAGAGAAGAAGAAGAGGAGGAGGAG39990.2342285248054392No Hit
GGTGAGTGCAAAGGGTTGCAACTGAGCTGCCTTCTGTCATTTATCTAGCT37990.22251416997645998No Hit
AAACAAGGTGGAGTAGGGGCTGGTGGGAGAAGTGTGTGATGTGGACCATG34260.2006668982204138No Hit
GAGATAAGGAGGGAGGATGGGAAAGAAAGGGAGGGGAGGAGAGGTCAAGG31530.18467680387885715No Hit
GCAAAAGCAGGGAGACAGGGAGGGAATCTGGGACTGAGAGGGGGAGGGGA29610.17343102324303714No Hit
GAGCGAAAGCAGGGAGACAGGGAGGGAATCTGGGACTGAGAGGGGGAGGG26470.15503948616153979No Hit
GAGCAAAAGCAGGGGTACCCTTGTAAGAAATTTTCACCACAGAAGGAGCC25030.14660515068467475No Hit
GTAATGGGCCCTGCTCCTTTGTGAGACAAAGGTGAGTGCAAAGGGTTGCA22860.13389507569523232No Hit
AGCAAAAGCAGGGAGACAGGGAGGGAATCTGGGACTGAGAGGGGGAGGGG22400.1312007740845671No Hit
GGTGGGAGAAGTGTGTGATGTGGACCATGGTAAAAGCTACAGTAATGGGC22070.12926790553778553No Hit
AGTAGAAACAAGGTGGAGTAGGGGCTGGTGGGAGAAGTGTGTGATGTGGA20610.12071642651263072No Hit
ATCTGGGACTGAGAGGGGGAGGGGAATGTTTGGAGGCAGAGAGAGAGAGG19720.11550353861373497No Hit
GGGTTGCAACTGAGCTGCCTTCTGTCATTTATCTAGCTTTGCCTTCCCTC19700.11538639506544517No Hit
GTGGAGTAGGGGCTGGTGGGAGAAGTGTGTGATGTGGACCATGGTAAAAG17820.10437490152620471No Hit
GTAGAAACAAGGTGGAGTAGGGGCTGGTGGGAGAAGTGTGTGATGTGGAC17570.10291060717258231No Hit
AGGTGGAGTAGGGGCTGGTGGGAGAAGTGTGTGATGTGGACCATGGTAAA17330.1015048845931048No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA82700.0106.6393361
GAGCGAA23550.097.0083161
AGCAAAA91100.096.3062742
CAAAAGC101900.086.099134
GCAAAAG101750.086.083533
AACGCGA300.001936225972.49766145
AAAAGCA122200.071.855585
AAAGCAG171350.064.743316
AGCGAAA35200.064.680672
GCGAAAG36250.062.607143
CGAAAGC37300.060.8447464
AAGCAGG186600.060.337017
AGCAGGC63950.053.1687288
GCAGGCC24850.053.0968749
AGCAGGG118400.046.4744268
AGCAGGT33950.039.291818
CCGGTGT750.001482848738.671084
GCAGGGA85750.035.762699
GGAGCGA5100.034.1295361
AACAAGG33700.033.779822
Produced by FastQC (version 0.11.5)