FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l02_n01_KO_PBS_F1_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l02_n01_KO_PBS_F1_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1399090
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGAGACAGGGAGGGAATCTGGGACTGAGAGGGGGAGGG63500.45386644175857166No Hit
GAGCAAAAGCAGGCCCAAGCAGCCAAAGAGAAGAAGAAGAGGAGGAGGAG43010.3074141048824593No Hit
GAGCAAAAGCAGGGGTACCCTTGTAAGAAATTTTCACCACAGAAGGAGCC27870.19920090916238412No Hit
GAGTAGGGGCTGGTGGGAGAAGTGTGTGATGTGGACCATGGTAAAAGCTA26690.1907668555989965No Hit
AAACAAGGTGGAGTAGGGGCTGGTGGGAGAAGTGTGTGATGTGGACCATG21490.15359983989593234No Hit
GGTGAGTGCAAAGGGTTGCAACTGAGCTGCCTTCTGTCATTTATCTAGCT20720.14809626257067093No Hit
GAGCAAAAGCAGGCTGCACCACGCTGCACAGCGGCAAAGGATGGTGATGA18420.13165700562508487No Hit
GCAAAAGCAGGGAGACAGGGAGGGAATCTGGGACTGAGAGGGGGAGGGGA17640.12608195326962526No Hit
GTAATGGGCCCTGCTCCTTTGTGAGACAAAGGTGAGTGCAAAGGGTTGCA15370.1098571214146338No Hit
GAGCGAAAGCAGGCCCAAGCAGCCAAAGAGAAGAAGAAGAGGAGGAGGAG14810.10585451972353459No Hit
GAGCGAAAGCAGGGAGACAGGGAGGGAATCTGGGACTGAGAGGGGGAGGG14610.10442501911957058No Hit
GTTATTATACAGGGAAACCATCCATTCCAGGCCCTGGAGCTGGTAATGCT14260.10192339306263358No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA15400.0103.610381
GAGCAAA67600.099.993211
AGCAAAA70200.095.76292
GCAAAAG76000.088.7409443
CAAAAGC80450.084.012634
AAAAGCA98900.068.706535
AGCGAAA23600.065.759192
GCGAAAG24350.064.32943
CGAAAGC25250.062.898094
TACGGGA350.003563005762.159562
AAAGCAG135600.062.032886
GCAGGCC21750.061.6656049
AAGCAGG141200.059.5085337
AGCAGGC49550.058.8183568
CGGGTTG502.0085643E-458.0114366
CGGGAGT553.2151793E-452.7414444
AGCAGGG79600.047.5431488
AGCAGGT24100.041.2129828
GCAGGTG16500.036.0296829
AACAAGG23350.033.8527762
Produced by FastQC (version 0.11.5)