FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l02_n01_KO_DI244_F1_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l02_n01_KO_DI244_F1_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3035154
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAA557931.8382263305255682No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG289970.9553716220000699No Hit
AATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA212190.6991078541648957No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT168530.5552601284811248No Hit
GAGCGAAAGCAGGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAA161680.5326912571816784No Hit
AGCGAAAGCAGGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAAG158100.5208961390426977No Hit
GCAAAAGCAGGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAAGA136330.4491699597450409No Hit
TCCTCATTCTGGGCAAAGAGGACAAGAGATATGGGCCAGCACTAAGCATC135520.4465012318979531No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT133790.44080135637269147No Hit
GAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATA132720.4372759998339458No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACATCCCCTTGC127760.42093416017770435No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT118500.39042499985173734No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCC118450.3902602635648801No Hit
AGCAAAAGCAGGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAAG109790.36172793868120034No Hit
AAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCCGAAT109560.36097015176165687No Hit
GGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATG107510.3542159640005087No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC99850.32897836485397447No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT99100.32650732055111537No Hit
GAATGAGGAATCCCCTCAGTCTTCTCCTGTCTTCCTGATGTGTACTTCTT96960.3194566074736241No Hit
TCAATGAACTGAGCAACCTTGCGAAAGGAGAGAAGGCTAATGTGCTAATT95270.31388852097784825No Hit
ATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAAAACCACCGTG88730.2923410146569169No Hit
GTTAAGGACAGAAGCCCTTATAGGGCCTTAATGAGCTGCCCTGTCGGTGA85990.28331346613713837No Hit
GTCCTTAACAGTCCCATTTGAATGCTTGTCATTCAGTAAGGCACCTTGGG80970.2667739429366681No Hit
ATATACAGCAAAGACAATAGCATAAGAATTGGTTCCAAAGGAGACGTTTT78500.2586359703659188No Hit
GTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCGCTATACCCTG78030.2570874492694605No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACAT76900.2533644091864861No Hit
GTCTTCTCCTGTCTTCCTGATGTGTACTTCTTGATTATGGCCATATGGTC75780.2496743163608832No Hit
GTGCTGGCCCATATCTCTTGTCCTCTTTGCCCAGAATGAGGAATCCCCTC75310.2481257952644248No Hit
GTTCATTGATGCTTAGTGCTGGCCCATATCTCTTGTCCTCTTTGCCCAGA75140.2475656918891101No Hit
GTATATAGCCCACCCACGGATGGGACAAAGAGATGAATTGCCGGTTAATA74760.24631369610899478No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA74420.24519348935836535No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG71420.23530931214692896No Hit
ATCAAGAAGTACACATCAGGAAGACAGGAGAAGACTGAGGGGATTCCTCA70550.23244290075561239No Hit
ATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTC70460.2321463754392693No Hit
GGAGAAGACTGAGGGGATTCCTCATTCTGGGCAAAGAGGACAAGAGATAT70400.23194869189504058No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA67940.22384366658166274No Hit
AGTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATC67370.22196567291148983No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA66440.21890157797594456No Hit
ATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTC62120.20466836279147615No Hit
GCTTAGTGCTGGCCCATATCTCTTGTCCTCTTTGCCCAGAATGAGGAATC59950.1975188079418705No Hit
GGTATGGTAATGGTGTTTGGATAGGAAGGACCAAAAGTCACAGTTCCAGA58860.19392755688838195No Hit
GTCTCGCACCCGCGAGATACTCACAAAAACCACCGTGGACCATATGGCCA57260.1886559957089492No Hit
GGCCAGCACTAAGCATCAATGAACTGAGCAACCTTGCGAAAGGAGAGAAG55320.182264227778887No Hit
TCGCAAGGTTGCTCAGTTCATTGATGCTTAGTGCTGGCCCATATCTCTTG54420.1792989746154561No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA54030.17801403157796936No Hit
ATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACC53740.17705856111419718No Hit
ATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACATCCCCTTGCCC53250.1754441455029959No Hit
CCACATCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTCGCAAGG52380.17257773411167934No Hit
GCACTAAGCATCAATGAACTGAGCAACCTTGCGAAAGGAGAGAAGGCTAA51500.16967837546299133No Hit
GCTGTATATAGCCCACCCACGGATGGGACAAAGAGATGAATTGCCGGTTA51270.16892058854344788No Hit
GTATTAAAATACAACGGCATAATAACTGAAACCATAAAAAGTTGGAGGAA50260.16559291554893096No Hit
ATATAGCCCACCCACGGATGGGACAAAGAGATGAATTGCCGGTTAATATC49670.16364902736401513No Hit
AATTAGCACATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGA49070.16167219192172788No Hit
ACCAACACCACATCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTT49050.16160629740698496No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA48720.16051903791372693No Hit
GCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCATTG48110.15850925521406822No Hit
GCCTCATACAGTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCG47500.1564994725144095No Hit
CTTCTTGATTATGGCCATATGGTCCACGGTGGTTTTTGTGAGTATCTCGC47390.15613705268332348No Hit
GGATTAGCCATTCAATTCAAACTGGAAGTCAAAACCATACTGGAATATGC47330.15593936913909476No Hit
CAATATGGATTAGCCATTCAATTCAAACTGGAAGTCAAAACCATACTGGA47240.15564284382275168No Hit
TACCATACCTATATGAAAATCCCTTTACTCCGTTTGCTCCATCAACATAC45260.14911928686320364No Hit
GTGTAAATGGTTCATGTTTTACTATAATGACTGATGGCCCGAGTGATGGG43190.14229920458731254No Hit
TTTTTGAACAGACTACTTGTCAATGCTGAATGGCAACTCAGCACCGTCTG43180.14226625732994108No Hit
CACCACATCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTCGCAA41520.1367970126062796No Hit
GACTACTTGTCAATGCTGAATGGCAACTCAGCACCGTCTGGCCAAGACCA40440.1332387088101625No Hit
GAAGCAGTTTTCTTGATTCAGCTGAAAATCCTTCTAGTTGTGGAGATGCA40440.1332387088101625No Hit
AAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACATCCCCTT39960.13165724045633267No Hit
TATCTCTTGTCCTCTTTGCCCAGAATGAGGAATCCCCTCAGTCTTCTCCT39280.12941682695507378No Hit
TTCCTGATGTGTACTTCTTGATTATGGCCATATGGTCCACGGTGGTTTTT39070.12872493455027323No Hit
ACTCAGCACCGTCTGGCCAAGACCAATCTACAGTATCACTATTCACGCCA37520.12361810965769776No Hit
GCCATATGGTCCACGGTGGTTTTTGTGAGTATCTCGCGGGTGCGAGACTG37360.1230909535397545No Hit
GTACACATCAGGAAGACAGGAGAAGACTGAGGGGATTCCTCATTCTGGGC37250.12272853370866849No Hit
CTCTTGTCCTCTTTGCCCAGAATGAGGAATCCCCTCAGTCTTCTCCTGTC37200.12256379742181121No Hit
CATCAGGAAGACAGGAGAAGACTGAGGGGATTCCTCATTCTGGGCAAAGA36930.12167422147278194No Hit
GTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAA36600.12058696197952395No Hit
GGGTAAAGGACACAACTTCAGTGATATTAACCGGCAATTCATCTCTTTGT36040.11874191556672249No Hit
CATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGATGCTTAGT36040.11874191556672249No Hit
CCATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAAGACTG35910.11831360122089357No Hit
GATGTGTACTTCTTGATTATGGCCATATGGTCCACGGTGGTTTTTGTGAG35650.11745697252923575No Hit
ACATACACTGGACCACAGCTGCCTGTTCCATCTTCGGGACGCGGGTTGTC35350.11646855480809211No Hit
ATCAAATAGGATACATCTGCAGTGGGGTTTTCGGTGACAACCCGCGTCCC35250.11613908223437755No Hit
GGATACATCTGCAGTGGGGTTTTCGGTGACAACCCGCGTCCCGAAGATGG35090.1156119261164343No Hit
TTACCATACCTATATGAAAATCCCTTTACTCCGTTTGCTCCATCAACATA34850.11482119193951938No Hit
CCATTTACACAGGCACATTCAGACTCTTGTGTCCTCAATATTTTCTTCCT34700.11432698307894755No Hit
CTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGG34550.11383277421837573No Hit
GGGCTAGACTGTATGAGGCCGTGCTTCTGGGTTGAATTAATCAGGGGACG34010.1120536223203172No Hit
GTATGAGGCCGTGCTTCTGGGTTGAATTAATCAGGGGACGACCTAAAGAA32920.10846237126682864No Hit
GTCTGGTAGTCGGACTAATTAGCCTAATATTGCAAATAGGGAATATAATC32880.10833058223734282No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG32820.10813289869311409No Hit
GTGTATGTTGATGGAGCAAACGGAGTAAAGGGATTTTCATATAGGTATGG32540.10721037548671336No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT32530.10717742822934191No Hit
CTAGCATACTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGGCC32480.10701269194248464No Hit
GTGCTAATTGGGCAAGGGGATGTGGTGTTGGTAATGAAACGGAAACGGGA32420.10681500839825592No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC31890.10506880375756882No Hit
TAATCAAGAAGTACACATCAGGAAGACAGGAGAAGACTGAGGGGATTCCT31850.10493701472808299No Hit
GTCTGGAACTGTGACTTTTGGTCCTTCCTATCCAAACACCATTACCATAC31840.10490406747071153No Hit
GCCTAATATTGCAAATAGGGAATATAATCTCAATATGGATTAGCCATTCA31800.10477227844122572No Hit
GGCACATTCAGACTCTTGTGTCCTCAATATTTTCTTCCTCCAACTTTTTA31790.10473933118385426No Hit
GTCTCTGTCCATCCATTAGGATCCCAAATCATCTCAAACCCATGTCTGGA31670.1043439640953968No Hit
TCCCCGTACAATTCAAGATTTGAATCGGTTGCTTGGTCAGCAAGTGCATG31500.10378386072008208No Hit
ATTAAAATACAACGGCATAATAACTGAAACCATAAAAAGTTGGAGGAAGA31210.1028283902563099No Hit
CTATTGATTTAGTAACCTTCCCCTTTTCGATCTTGAAAATTTTGTACGAG31110.10249891768259534No Hit
GAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAA30990.10210355059413788No Hit
AACATACACTGGACCACAGCTGCCTGTTCCATCTTCGGGACGCGGGTTGT30650.10098334384350843No Hit
ATTAGCACATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGAT30580.10075271304190825No Hit
CATCTGCAGTGGGGTTTTCGGTGACAACCCGCGTCCCGAAGATGGAACAG30550.10065387126979389No Hit
TCTCCTGTCTTCCTGATGTGTACTTCTTGATTATGGCCATATGGTCCACG30530.10058797675505098No Hit
TAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAA30510.10052208224030808No Hit
GGAATATGCAACCAAAACATCATTACCTATAAAAATAGCACCTGGGTAAA30410.10019260966659352No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG100.0070868293145.032421
GAGCGAA24850.0140.9471
GCAAAAG142100.099.5022053
CAAAAGC150000.094.261764
AAAAGCA160600.087.99515
TACGGAT259.3839504E-487.010864
ACGCTAG259.3839504E-487.010863
GCAGGTC121700.086.557149
AAGCAGG205650.085.665387
GCAGGGG35550.085.448059
AGCAAAA168500.084.2568052
GAGCAAA168750.083.925441
TATTAGT1750.082.875681
AGCAGGT166750.082.258948
AGCAGGC2050.081.3395548
TCTAAAC451.2325781E-680.5656053
GCAGGGT8750.077.883889
AAAGCAG231550.076.15726
ATTAGTA4700.075.6020051
GTCAATT1650.074.706292
Produced by FastQC (version 0.11.5)