FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l02_n01_KO_Control_M_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l02_n01_KO_Control_M_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2045085
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGAGACAGGGAGGGAATCTGGGACTGAGAGGGGGAGGG181300.8865157193955263No Hit
GAGTAGGGGCTGGTGGGAGAAGTGTGTGATGTGGACCATGGTAAAAGCTA85790.4194935662820861No Hit
GAGCAAAAGCAGGCCCAAGCAGCCAAAGAGAAGAAGAAGAGGAGGAGGAG71210.3482006860350548No Hit
AAACAAGGTGGAGTAGGGGCTGGTGGGAGAAGTGTGTGATGTGGACCATG61430.30037871286523543No Hit
GGTGAGTGCAAAGGGTTGCAACTGAGCTGCCTTCTGTCATTTATCTAGCT55810.27289819249566644No Hit
GAGCAAAAGCAGGGGTACCCTTGTAAGAAATTTTCACCACAGAAGGAGCC48850.2388653772337091No Hit
GCAAAAGCAGGGAGACAGGGAGGGAATCTGGGACTGAGAGGGGGAGGGGA47840.2339267072028791No Hit
GAGCGAAAGCAGGGAGACAGGGAGGGAATCTGGGACTGAGAGGGGGAGGG43880.21456320886417923No Hit
GGTGGGAGAAGTGTGTGATGTGGACCATGGTAAAAGCTACAGTAATGGGC40310.19710672172550286No Hit
GTAATGGGCCCTGCTCCTTTGTGAGACAAAGGTGAGTGCAAAGGGTTGCA40060.19588427864856472No Hit
AGCGAAAGCAGGGAGACAGGGAGGGAATCTGGGACTGAGAGGGGGAGGGG37440.18307307520225322No Hit
ATCTGGGACTGAGAGGGGGAGGGGAATGTTTGGAGGCAGAGAGAGAGAGG35690.17451597366368635No Hit
AGCAAAAGCAGGGAGACAGGGAGGGAATCTGGGACTGAGAGGGGGAGGGG35520.17368471237136843No Hit
AGTAGAAACAAGGTGGAGTAGGGGCTGGTGGGAGAAGTGTGTGATGTGGA35410.17314683741751566No Hit
GAGATAAGGAGGGAGGATGGGAAAGAAAGGGAGGGGAGGAGAGGTCAAGG35150.1718754966175No Hit
AGGTGGAGTAGGGGCTGGTGGGAGAAGTGTGTGATGTGGACCATGGTAAA31630.15466349809421123No Hit
GGGTTGCAACTGAGCTGCCTTCTGTCATTTATCTAGCTTTGCCTTCCCTC31550.15427231630959104No Hit
GTGGAGTAGGGGCTGGTGGGAGAAGTGTGTGATGTGGACCATGGTAAAAG29550.14449277169408606No Hit
GTAGAAACAAGGTGGAGTAGGGGCTGGTGGGAGAAGTGTGTGATGTGGAC29430.14390599901715578No Hit
GTTATTATACAGGGAAACCATCCATTCCAGGCCCTGGAGCTGGTAATGCT28790.14077654474019416No Hit
CAGGGAGACAGGGAGGGAATCTGGGACTGAGAGGGGGAGGGGAATGTTTG26340.12879660258620057No Hit
GTATAATAACAATCTGAACGGAATCTTAGCTGATGAAATGGGGCTAGGCA26270.12845431852465788No Hit
GAGACAGGGAGGGAATCTGGGACTGAGAGGGGGAGGGGAATGTTTGGAGG25820.12625392098616928No Hit
TCCTTGACCTCTCCTCCCCTCCCTTTCTTTCCCATCCTCCCTCCTTATCT25120.12283108037074253No Hit
GAGCGAAAGCAGGCCCAAGCAGCCAAAGAGAAGAAGAAGAGGAGGAGGAG24740.1209729668937966No Hit
AAAGCAGGGAGACAGGGAGGGAATCTGGGACTGAGAGGGGGAGGGGAATG23780.11627878547835421No Hit
GAGCAAAAGCAGGCAAAAGTGTCAGAGACAGCCTCTGCTCCCGCTGTTAG23100.11295374030908252No Hit
GAGCAAAAGCAGGAGGGATCAAGTAAGAGGGAGATGGAGGGAGAAATTAT23060.1127581494167724No Hit
GTACTGCATGCTGTATTCGTCGTCCACGTCCTGCTTCGCAGTCTCAATGA22050.1078194793859424No Hit
TTATTATACAGGGAAACCATCCATTCCAGGCCCTGGAGCTGGTAATGCTT21910.10713491126285704No Hit
GGTAAAAGCTACAGTAATGGGCCCTGCTCCTTTGTGAGACAAAGGTGAGT21850.1068415249243919No Hit
GATGTGGACCATGGTAAAAGCTACAGTAATGGGCCCTGCTCCTTTGTGAG21680.10601026363207397No Hit
CCTTATCTCCCTCCATGTGTCTTCCCCTCCCTCACTCTCAGCCCCATCTT21430.10478782055513584No Hit
GTGGGAGAAGTGTGTGATGTGGACCATGGTAAAAGCTACAGTAATGGGCC20780.10160946855509673No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA25800.0117.22871
GAGCAAA108700.0113.765991
AGCAAAA118400.0104.236362
CAAAAGC133800.092.4016344
GCAAAAG139500.088.730043
AAAAGCA149400.083.044495
AGCGAAA42650.070.387042
AAAGCAG227500.067.667486
GCGAAAG45450.066.0507663
AAGCAGG241600.064.396057
GCAGGCC29750.064.334429
CGAAAGC47550.063.286194
AGCAGGC80550.058.3224648
AGTAGAA21650.050.9217451
AGCAGGT36200.048.4655578
AGCAGGG158900.047.9974638
GCAGGTG30050.043.667729
GTAGAAA26150.042.1486132
GGAGCGA3500.041.4457051
AACAAGG42750.040.1997762
Produced by FastQC (version 0.11.5)