FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l02_n01_DI244G4_P3CW_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l02_n01_DI244G4_P3CW_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3523643
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG182540.5180433999698608No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG140760.39947293184922533No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC137610.39053332020298315No Hit
AATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA136330.38690071610546245No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT129280.36689301384958695No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA127950.3631185111545069No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAA104520.2966248283381716No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT97550.2768441638383911No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA95920.27221826955795464No Hit
TCCTCATTCTGGGCAAAGAGGACAAGAGATATGGGCCAGCACTAAGCATC91530.25975957269223926No Hit
GAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATA90020.25547423504594535No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT85230.24188034939975475No Hit
AGCGAAAGCAGGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAAG82680.2346435209242253No Hit
GATGTAAGGCTTGCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATC80850.22945003225355123No Hit
AGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGC79490.2255903903999355No Hit
GTAGAAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTT78350.22235510237558118No Hit
TGATAACACAGTTCGAGTCTCTGAAACTCTACAGAGATTCGCTTGGAGAA77530.220027965375607No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCC76580.21733189202197842No Hit
ATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAAAACCACCGTG72560.20592324477820256No Hit
GGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATG71440.202744716192872No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT70990.20146762881483737No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT68750.19511057164417622No Hit
CCTCAGCAGTATGTCCTGGAAGAGAAGGCAATGGTGAAATTTCGCCAACA67960.19286857380273767No Hit
GACATAATGGATCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCT67210.19074009483934667No Hit
AAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCCGAAT66090.1875615662540161No Hit
GGTCCATGCTCATACCCAAGCAGAAAGTGGCAGGCCCTCTTTGTATCAGA65370.18551822644916072No Hit
CTACAGAGATTCGCTTGGAGAAGCAGTAATGAGAATGGGAGACCTCCACT64460.18293567197357963No Hit
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT62000.1759542609736571No Hit
TCAATGAACTGAGCAACCTTGCGAAAGGAGAGAAGGCTAATGTGCTAATT61010.17314466874198098No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACATCCCCTTGC60640.1720946191200414No Hit
GATACAAAGAGGGCCTGCCACTTTCTGCTTGGGTATGAGCATGGACCAGT59890.16996614015665037No Hit
TCTCTGTAGAGTTTCAGAGACTCGAACTGTGTTATCATTCCATTCAAGTC59510.16888771081519893No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA57490.16315500747379913No Hit
CTCTTGCTCCACTTCAAGCAATAGATGTAAGGCTTGCATAAATGTTATTT56790.16116842710796753No Hit
GAATGGACCAGGCGATCATGGATAAGAACATCATACTGAAAGCGAACTTC55940.15875615094945772No Hit
TCTTATTTCTTCAAACTTCTGACCTAATTGTTCCCGCCATTTCTCGTTTC55920.15869939151043394No Hit
GTTCTTATCTCTTGCTCCACTTCAAGCAATAGATGTAAGGCTTGCATAAA55760.15824531599824387No Hit
GAGTGGAGGTCTCCCATTCTCATTACTGCTTCTCCAAGCGAATCTCTGTA54050.15339238396171237No Hit
GTTTTGGAGTGAGTGGAGGTCTCCCATTCTCATTACTGCTTCTCCAAGCG54010.1532788650836648No Hit
CTGCTGAGGATGTCAAAAATGCAGTTGGAGTCCTCATCGGGGGACTTGAA53930.15305182732756978No Hit
GATAAGAACATCATACTGAAAGCGAACTTCAGTGTGATTTTTGACCGGCT53070.15061117144954808No Hit
GAATGAGGAATCCCCTCAGTCTTCTCCTGTCTTCCTGATGTGTACTTCTT52430.14879486940078776No Hit
CTTTGTATCAGAATGGACCAGGCGATCATGGATAAGAACATCATACTGAA51790.14697856735202744No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA50910.14448115203498196No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT50540.1434311024130424No Hit
CTCCAAAACAGAAACGAGAAATGGCGGGAACAATTAGGTCAGAAGTTTGA49870.14152966120574642No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA49810.14135938288867517No Hit
ACCTAACTGACATGACTCTTGAGGAAATGTCAAGGGACTGGTCCATGCTC49160.13951470112040296No Hit
AAACTATTCTCTGTTATCTTCAGTTTGTGTCTCACTTCTTCAATCAACCA48690.13818085430334456No Hit
ATGTAAGGCTTGCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATCT48670.1381240948643208No Hit
GGTACAGAGGCCATGGTCATTTTAAGTGCCTCATCGGATTCTTCTTTCAG47520.1348604271204546No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG46920.1331576439497418No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG46900.13310088451071803No Hit
TCTCATTACTGCTTCTCCAAGCGAATCTCTGTAGAGTTTCAGAGACTCGA46310.1314264810595171No Hit
GGAGAAGACTGAGGGGATTCCTCATTCTGGGCAAAGAGGACAAGAGATAT45500.1291277237790548No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACAT45450.1289858251814954No Hit
GGTCCATTCTGATACAAAGAGGGCCTGCCACTTTCTGCTTGGGTATGAGC45320.12861688882784097No Hit
CTGTACCTGCGTCGCGTTACCTAACTGACATGACTCTTGAGGAAATGTCA44820.12719790285224694No Hit
TCCTAGCTCCAGTGCTGGTCTGAAAAATGATCTTCTTGAAAATTTGCAGG44820.12719790285224694No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA44480.126232992388843No Hit
GTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCCGCAAACGAGT44210.12546673996202226No Hit
CCTCCACTCACTCCAAAACAGAAACGAGAAATGGCGGGAACAATTAGGTC43960.12475724697422527No Hit
GTTCTTATCCATGATCGCCTGGTCCATTCTGATACAAAGAGGGCCTGCCA43820.12435993090105894No Hit
CTTCCAGGACATACTGCTGAGGATGTCAAAAATGCAGTTGGAGTCCTCAT43740.12413289314496388No Hit
GTCTCGCACCCGCGAGATACTCACAAAAACCACCGTGGACCATATGGCCA43480.12339502043765502No Hit
GAACTAGGTGATGCCCCATTCCTTGATCGGCTTCGCCGAGATCAGAAATC43400.12316798268155996No Hit
ATCAAGAAGTACACATCAGGAAGACAGGAGAAGACTGAGGGGATTCCTCA43290.12285580576692928No Hit
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCTGCTGT43160.12248686941327486No Hit
AGTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATC42440.12044352960841948No Hit
GCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGCT42280.11998945409622938No Hit
GCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATTTCACCAT41840.11874074643770667No Hit
GTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGTATGATGTT41640.11817315204746906No Hit
CTTAAAATGACCATGGCCTCTGTACCTGCGTCGCGTTACCTAACTGACAT41360.11737851990113642No Hit
CATTACTGCTTCTCCAAGCGAATCTCTGTAGAGTTTCAGAGACTCGAACT40900.11607305280358993No Hit
GTCGTACTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA40890.11604467308407804No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT40730.11559059757188797No Hit
ATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTC40710.11553383813286419No Hit
ACCTAATTGTTCCCGCCATTTCTCGTTTCTGTTTTGGAGTGAGTGGAGGT40450.11479596542555533No Hit
GTCTTCTCCTGTCTTCCTGATGTGTACTTCTTGATTATGGCCATATGGTC40420.11471082626701967No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCCCCGTTCCC40370.11456892766946027No Hit
CTTCTGACCTAATTGTTCCCGCCATTTCTCGTTTCTGTTTTGGAGTGAGT40280.11431351019385336No Hit
ATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTC39400.11181609487680788No Hit
GTTCATTGATGCTTAGTGCTGGCCCATATCTCTTGTCCTCTTTGCCCAGA38950.1105390074987733No Hit
GGTATGTGCAACCTGTGAACAGATTGCTGACTCCCAGCATCGGTCTCATA38920.11045386834023765No Hit
GTGCTGGCCCATATCTCTTGTCCTCTTTGCCCAGAATGAGGAATCCCCTC38920.11045386834023765No Hit
CTGTTATCTTCAGTTTGTGTCTCACTTCTTCAATCAACCATCTTATTTCT38440.1090916418036674No Hit
CTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAAT38100.10812673134026347No Hit
TCCTGGAAGAGAAGGCAATGGTGAAATTTCGCCAACAATTGCTCCCTCTT38040.10795645302319218No Hit
GCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATCTTCAGTTTGTGT37980.10778617470612092No Hit
TTCTGATACAAAGAGGGCCTGCCACTTTCTGCTTGGGTATGAGCATGGAC37490.1063955684500388No Hit
GCATGGACCAGTCCCTTGACATTTCCTCAAGAGTCATGTCAGTTAGGTAA37430.1062252901329675No Hit
GTCCATGCTCATACCCAAGCAGAAAGTGGCAGGCCCTCTTTGTATCAGAA37390.10611177125491998No Hit
GTTATCTTCAGTTTGTGTCTCACTTCTTCAATCAACCATCTTATTTCTTC37340.10596987265736058No Hit
CCCTAAGAGGAAGGGGCAGCACCCTCGGTCTGGACATCGAGACAGCCACA36840.10455088668176657No Hit
GTCCTGGAAGAGAAGGCAATGGTGAAATTTCGCCAACAATTGCTCCCTCT36660.10404005173055274No Hit
GAGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG36510.10361435593787452No Hit
AGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGC36260.10290486295007752No Hit
ATGATGTTCTTATCCATGATCGCCTGGTCCATTCTGATACAAAGAGGGCC36030.10225212940130428No Hit
CCTGTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGAT35440.10057772595010335No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACGG100.007092143144.9964145
GAGCGAA11350.0138.669891
AGCAAAA53150.096.865372
GCAAAAG54050.095.252453
AGCGAAA33400.094.2457051
ACTAATA3450.092.461478
CTAGGGG259.382249E-487.0151143
ATTAGTA4150.078.64711
TACTAAT4200.075.950497
TCACGCA300.0019363472.4982145
ACACGGT406.66634E-572.4982145
ATTAATA300.0019363472.4982145
AAGGGTG35150.066.63325
GCAGGGG7200.066.456689
CTACTAA4850.065.78376
ACAAGGG40100.065.641083
AGGGTGT36050.064.9687656
GCAGGGT48000.064.493199
CAAGGGT41150.063.966164
AGCAGGG57000.062.8317688
Produced by FastQC (version 0.11.5)