FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l02_n01_DI222G4_P2CW_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l02_n01_DI222G4_P2CW_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4119949
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA463491.124989653998144No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG458051.1117856070548446No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA437761.0625374246137513No Hit
AGCGAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAG248120.6022404646271107No Hit
GAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATA234840.5700070559125853No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT217520.5279677005710508No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCC194480.47204467822295854No Hit
ATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAAAACCACCGTG176800.42913152565723506No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT175220.4252965267288503No Hit
AAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCCGAAT171980.41743235171114984No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG165980.4028690646413342No Hit
TCAATGAACTGAGCAACCTTGCGAAAGGAGAGAAGGCTAATGTGCTAATT163740.3974321041352697No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTTGC162190.3936699216422339No Hit
GGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATG137620.3340332610913388No Hit
ATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACCCAGCACTTAGGAT133270.3234748779657224No Hit
GAGCGAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA127620.3097611159749793No Hit
GTTCATTGATGCTTAGTGCTGGCCCATATTTCCGTTATCCTCTTGTCTGC124420.30199402953774424No Hit
ATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTC123340.2993726378651774No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA121030.2937657723432984No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG120170.2916783678632915No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGT119270.2894938748028192No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA116400.282527769154424No Hit
GTCTCGCACCCGCGAGATACTCACAAAAACCACCGTGGACCATATGGCCA113920.27650827716556686No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT113790.2761927392790542No Hit
GCTTAGTGCTGGCCCATATTTCCGTTATCCTCTTGTCTGCTGTAATTGGA109000.26456638176831804No Hit
AGTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATC108200.26262461015900923No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC106460.2584012569087627No Hit
GCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAGA106410.25827989618318087No Hit
GCACTAAGCATCAATGAACTGAGCAACCTTGCGAAAGGAGAGAAGGCTAA101740.24694480441384103No Hit
GTGCTGGCCCATATTTCCGTTATCCTCTTGTCTGCTGTAATTGGATATTT98730.23963888873381683No Hit
TCGCAAGGTTGCTCAGTTCATTGATGCTTAGTGCTGGCCCATATTTCCGT98110.23813401573660256No Hit
ATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACC94710.22988148639704037No Hit
AGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAG89700.21772114169374426No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT87580.21257544692907604No Hit
ATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTTGCCC86140.20908025803232033No Hit
ATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTC85730.20808510008254957No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT85320.20708994213277884No Hit
GAAATATCCAATTACAGCAGACAAGAGGATAACGGAAATATGGGCCAGCA84400.20485690478207375No Hit
ATCCTCTTGTCTGCTGTAATTGGATATTTCATTGCCATCATCCATTTCAT83880.20359475323602308No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA83740.20325494320439402No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA82490.20022092506484912No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA81810.19857041919693666No Hit
GGCCAGCACTAAGCATCAATGAACTGAGCAACCTTGCGAAAGGAGAGAAG81650.1981820648750749No Hit
AATTAGCACATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGA79630.1932790915615703No Hit
CCATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACC78820.1913130478071452No Hit
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT77740.18869165613457836No Hit
CATCCATTTCATCCTAAGTGCTGGGTTCTTCTCCTGTCTTCCTGATGTGT74470.18075466468152884No Hit
GTACACATCAGGAAGACAGGAGAAGAACCCAGCACTTAGGATGAAATGGA74310.18036631035966708No Hit
ACCCAGCACTTAGGATGAAATGGATGATGGCAATGAAATATCCAATTACA74260.18024494963408527No Hit
GATGTAAGGCTTGCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATC72690.17643422285081684No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA72690.17643422285081684No Hit
GGATATTTCATTGCCATCATCCATTTCATCCTAAGTGCTGGGTTCTTCTC72280.1754390649010461No Hit
TGATAACACAGTTCGAGTCTCTGAAACTCTACAGAGATTCGCTTGGAGAA70770.17177397098847583No Hit
CATCAGGAAGACAGGAGAAGAACCCAGCACTTAGGATGAAATGGATGATG69800.16941957291218898No Hit
GTAGAAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTT69360.16835159852706916No Hit
CAATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCG69000.1674778013028802No Hit
GTGCTGGGTTCTTCTCCTGTCTTCCTGATGTGTACTTCTTGATTATGGCC68190.1655117575484551No Hit
AGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGC66110.16046315136425232No Hit
GCCATATGGTCCACGGTGGTTTTTGTGAGTATCTCGCGGGTGCGAGACTG65070.15793884827215093No Hit
CTTCTTGATTATGGCCATATGGTCCACGGTGGTTTTTGTGAGTATCTCGC63320.15369122287678805No Hit
GGTCCATGCTCATACCCAAGCAGAAAGTGGCAGGCCCTCTTTGTATCAGA62600.15194362842841017No Hit
CATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGATGCTTAGT62590.1519193562832938No Hit
CCTCAGCAGTATGTCCTGGAAGAGAAGGCAATGGTGAAATTTCGCCAACA61200.14854552811211982No Hit
AAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTT60700.14733192085630187No Hit
GTGCTAATTGGGCAAGGAGACGTGGTGTTGGTAATGAAACGGAAACGGGA60340.14645812363211294No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT59960.14553578211769128No Hit
GAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAA58270.14143378959302652No Hit
GGATGAAATGGATGATGGCAATGAAATATCCAATTACAGCAGACAAGAGG58050.14089980240046662No Hit
TCCTAGCTCCAGTGCTGGTCTGAAAAATGATCTTCTTGAAAATTTGCAGG57960.14068135309441937No Hit
CTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGG57440.13941920154836868No Hit
CACCACGTCTCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTCGCAA57000.13835122716324885No Hit
TAATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACCCAGCACTTAGG56740.13772015139022353No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC56680.13757451851952537No Hit
CTAGCATACTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGGCC56190.13638518340882375No Hit
GTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAA55830.1355113861846348No Hit
ACCAACACCACGTCTCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTT55300.13422496249346777No Hit
TTACCAACACCACGTCTCCTTGCCCAATTAGCACATTAGCCTTCTCTCCT54860.13315698810834795No Hit
GATGTGTACTTCTTGATTATGGCCATATGGTCCACGGTGGTTTTTGTGAG54740.13286572236695163No Hit
CTACAGAGATTCGCTTGGAGAAGCAGTAATGAGAATGGGAGACCTCCACT54660.13267154520602076No Hit
TCGCAGTCTCGCACCCGCGAGATACTCACAAAAACCACCGTGGACCATAT54410.13206474157811177No Hit
TTCCTGATGTGTACTTCTTGATTATGGCCATATGGTCCACGGTGGTTTTT54040.13116667220880646No Hit
GTAATTGGATATTTCATTGCCATCATCCATTTCATCCTAAGTGCTGGGTT53700.13034141927485024No Hit
TCCAATTACAGCAGACAAGAGGATAACGGAAATATGGGCCAGCACTAAGC53570.13002588138833757No Hit
ATTTCATCCTAAGTGCTGGGTTCTTCTCCTGTCTTCCTGATGTGTACTTC53340.1294676220506613No Hit
TTTCATTGCCATCATCCATTTCATCCTAAGTGCTGGGTTCTTCTCCTGTC53100.12888509056786868No Hit
AAATGGATGATGGCAATGAAATATCCAATTACAGCAGACAAGAGGATAAC53040.12873945769717052No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCCCCGTTCCC53040.12873945769717052No Hit
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCTGCTGT52250.12682195823297812No Hit
GACATAATGGATCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCT52200.12670059750739632No Hit
GAGTGGAGGTCTCCCATTCTCATTACTGCTTCTCCAAGCGAATCTCTGTA51720.12553553454181107No Hit
GAATGGACCAGGCGATCATGGATAAGAACATCATACTGAAAGCGAACTTC51620.1252928130906475No Hit
CTCTTGCTCCACTTCAAGCAATAGATGTAAGGCTTGCATAAATGTTATTT51140.12412775012506222No Hit
GATACAAAGAGGGCCTGCCACTTTCTGCTTGGGTATGAGCATGGACCAGT51110.12405493368971315No Hit
CCTCTTGTCTGCTGTAATTGGATATTTCATTGCCATCATCCATTTCATCC50960.12369085151296776No Hit
GAAGTACACATCAGGAAGACAGGAGAAGAACCCAGCACTTAGGATGAAAT50370.12225879495110256No Hit
TCTCTGTAGAGTTTCAGAGACTCGAACTGTGTTATCATTCCATTCAAGTC50280.12204034564505532No Hit
ATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACCCA49940.1212150927110991No Hit
CTGCTGAGGATGTCAAAAATGCAGTTGGAGTCCTCATCGGGGGACTTGAA49640.12048692835760831No Hit
GAGAAGAACCCAGCACTTAGGATGAAATGGATGATGGCAATGAAATATCC49620.12043838406737559No Hit
ATATTTCCGTTATCCTCTTGTCTGCTGTAATTGGATATTTCATTGCCATC49340.11975876400411752No Hit
AAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAAAACCA48910.11871506176411407No Hit
ACCTTGCGAAAGGAGAGAAGGCTAATGTGCTAATTGGGCAAGGAGACGTG48490.11769563166922697No Hit
GATAAGAACATCATACTGAAAGCGAACTTCAGTGTGATTTTTGACCGGCT48430.11754999879852882No Hit
GACAAGAGGATAACGGAAATATGGGCCAGCACTAAGCATCAATGAACTGA48210.11701601160596892No Hit
CTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGGCCATCAATTA47960.11640920797805993No Hit
GGTATGTGCAACCTGTGAACAGATTGCTGACTCCCAGCATCGGTCTCATA47560.11543832217340555No Hit
CTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACC47420.11509851214177652No Hit
GGAGAAGAACCCAGCACTTAGGATGAAATGGATGATGGCAATGAAATATC47190.11454025280410024No Hit
TACCAACACCACGTCTCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTT46710.11337518983851498No Hit
GTTCTTATCTCTTGCTCCACTTCAAGCAATAGATGTAAGGCTTGCATAAA46180.11208876614734795No Hit
ATTAGCACATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGAT46020.11170041182548618No Hit
GAAATGGATGATGGCAATGAAATATCCAATTACAGCAGACAAGAGGATAA45840.11126351321339172No Hit
GGTACAGAGGCCATGGTCATTTTAAGTGCCTCATCGGATTCTTCTTTCAG45010.10924892516873388No Hit
TCTTATTTCTTCAAACTTCTGACCTAATTGTTCCCGCCATTTCTCGTTTC45000.10922465302361753No Hit
CTTTGTATCAGAATGGACCAGGCGATCATGGATAAGAACATCATACTGAA44780.1086906658310576No Hit
AAACTATTCTCTGTTATCTTCAGTTTGTGTCTCACTTCTTCAATCAACCA44680.10844794437989402No Hit
ACCGTGGACCATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGA44610.10827803936407951No Hit
ATGTAAGGCTTGCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATCT44160.10718579283384333No Hit
GTTTTGGAGTGAGTGGAGGTCTCCCATTCTCATTACTGCTTCTCCAAGCG44110.10706443210826153No Hit
ACCTAACTGACATGACTCTTGAGGAAATGTCAAGGGACTGGTCCATGCTC43430.1054139262403491No Hit
TCTCATTACTGCTTCTCCAAGCGAATCTCTGTAGAGTTTCAGAGACTCGA42980.10432167971011293No Hit
CTCCAAAACAGAAACGAGAAATGGCGGGAACAATTAGGTCAGAAGTTTGA42900.10412750254918204No Hit
GCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGCT42840.10398186967848388No Hit
CAGGAAGACAGGAGAAGAACCCAGCACTTAGGATGAAATGGATGATGGCA42650.10352069892127305No Hit
AGGTTGCTCAGTTCATTGATGCTTAGTGCTGGCCCATATTTCCGTTATCC42500.10315661674452767No Hit
CTTCCAGGACATACTGCTGAGGATGTCAAAAATGCAGTTGGAGTCCTCAT42350.10279253456778228No Hit
CAAGAGGATAACGGAAATATGGGCCAGCACTAAGCATCAATGAACTGAGC42330.10274399027754955No Hit
GAGCAACCTTGCGAAAGGAGAGAAGGCTAATGTGCTAATTGGGCAAGGAG42270.10259835740685139No Hit
GGTCCATTCTGATACAAAGAGGGCCTGCCACTTTCTGCTTGGGTATGAGC41340.10034104791102998No Hit
TCTTGATTATGGCCATATGGTCCACGGTGGTTTTTGTGAGTATCTCGCGG41250.10012259860498274No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA21300.0139.614211
AGCAAAA101750.0111.741822
GCAAAAG115800.097.996353
AGCGAAA59000.091.9549261
CAAAAGC142750.079.6986544
TATTAGT2800.077.71211
CGAAAGC72650.074.8565443
ATTAAGT300.001935003672.511044
AAGTAGG300.001936308372.498727
ACTAATA5950.071.889498
GCAGGGT52850.070.646819
AGTAGAA39550.070.0555041
AGCAGGG59000.069.67258
AAGCAGG209100.068.511477
AGCAGGC5750.068.0857548
GCAGGGG5200.066.921899
GCAGGTC124700.066.917429
TACTAAT6400.066.8347557
ATTAGTA5650.066.754461
AAAAGCA173150.065.8315665
Produced by FastQC (version 0.11.5)