FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10513127
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA20400.069.710411
CGAAAGC39600.060.6451843
GAGCAAA139000.050.8934171
GCAAAAG139400.050.4341243
AGCAAAA168200.041.754432
CAAAAGC184350.038.7271424
TATCGTG13350.037.499112
AGCAGGG112250.031.398348
GCAGGTA59400.031.3782169
ATCGTGT16050.029.3833853
AAAGCAG289050.029.2295536
ATATCGT16450.029.110511
AAGCAGG290250.029.1077377
AGCGAAA87700.028.2942561
AAAAGCA256100.028.045845
GGTAGTT16800.024.6048267
CACGAGC7050.023.6589538
ACAAGGT36400.023.3210433
TCTCGGG33950.023.059542145
GCAGGGT92550.022.4115149
Produced by FastQC (version 0.11.5)