FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n02_SAMPLE_6_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n02_SAMPLE_6_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3029787
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG48920.1614634956186689No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA46830.15456532092850092No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT43580.14383849425718706No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT35790.11812711586656091No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAAACCTACT35700.11783006528181685No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA35270.1164108235991507No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC34080.11248315475642348No Hit
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT33940.1120210760690438No Hit
TTCTTACACTTTCCATGCATTCATTGTCACACTTGTGGTAGAACTCAAAA33800.11155899738166412No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA32370.10683919364628602No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA5250.0125.761111
GCAAAAG36050.097.408573
AGCGAAA13300.086.192541
GCAGGGT19450.070.097369
GTCAATT5150.069.031292
AAGGGTG13550.066.927765
GCAGGGG15250.066.57669
AGGGTGT13550.065.8297356
ACAAGGG16000.065.297963
CAAGGGT16500.065.07814
ACGGCCC451.1951754E-464.43433145
ACTGGCC902.8376235E-1064.43433145
TCTCGGG8450.061.765457145
TAAACTA7300.060.623725
CGGTGGG11900.057.869076145
AGCAAAA62800.056.3789862
CTATTCG8000.056.203739
CCTGTTT1301.8189894E-1255.760483145
TTTTTAA8050.054.9791761
AGGTGTT3300.054.91563145
Produced by FastQC (version 0.11.5)