FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n02_SAMPLE_4_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n02_SAMPLE_4_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3535459
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG56110.1587064084182563No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT54180.15324742841028563No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA52630.14886327348160452No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA43950.12431200588099028No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAAACCTACT41850.11837218307439006No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC39260.11104640161291647No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT39150.11073526803733263No Hit
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT38540.10900989093636781No Hit
TTCTTACACTTTCCATGCATTCATTGTCACACTTGTGGTAGAACTCAAAA37580.10629454336763629No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT35990.10179724895692469No Hit
GAGTAGAGGCTTTGGGTCCGGCATCATCACCTCAAACGCATCAATGCATG35490.10038300543154367No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGGG151.2309443E-4144.97997145
GAGCGAA5650.0134.828411
GCAAAAG39750.094.723663
AGCGAAA15150.086.198651
TTCCGCC353.440842E-582.8457145
CCTCGCC703.092282E-1182.8457145
GTCAATT5900.072.5464252
GCAGGGG17750.071.501369
TGACGCC5800.069.99033145
GCAGGGT20800.069.036139
TCTCGGG9400.067.0918145
AAGGGTG12200.065.4107065
ACAAGGG14900.064.758033
AGGGTGT12550.063.5622146
CAAGGGT15350.063.3322144
ACGGCCC350.003569204162.134274145
GACGCTC1450.059.99171145
GTCGCCC5150.059.118046145
TAAACTA8050.058.5778545
AGCAGGC3600.058.416198
Produced by FastQC (version 0.11.5)