FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n02_SAMPLE_3_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n02_SAMPLE_3_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2733409
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG57340.2097746806277436No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA57040.20867715003499293No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT39160.14326432670705336No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAAACCTACT38760.1418009525833858No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT34820.12738671746526042No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT32590.11922840672581381No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA32340.11831379789852159No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC31510.11527729659191142No Hit
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT29350.10737507632410663No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA28990.10605803961280585No Hit
GGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATG28090.10276544783455385No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT28020.10250935736291203No Hit
CTCCTATTGTGACTGGGTGTATATTCTGGTAAGGGAGACTGCTGTTTATA27750.10152157982943642No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTGA100.0070940927144.98248145
GAGCGAA7700.0128.132581
GCAAAAG41350.0101.4004443
GCAGGGG18450.083.725839
GCAGGGT20700.077.077679
AGCAGGC3100.074.851578
ACTAATA1850.070.5526668
TACTAAT1850.070.5526667
CCTCGCC955.456968E-1268.67591145
AGCGAAA14650.067.34122
AGCAGGG48450.064.804748
AGCAAAA65300.064.764192
AAGCAGG81650.062.9655047
GTCAATT5600.059.5867042
TCTCGGG7500.058.95954145
CAAAAGC73500.057.638554
AGGGTGT10700.056.930886
CTACTAA2300.056.7540786
CAAGGGT13650.056.332724
ACAAGGG13650.056.332723
Produced by FastQC (version 0.11.5)