FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n02_SAMPLE_2_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n02_SAMPLE_2_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3177582
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG58250.18331548957666555No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA56100.17654933845924353No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT42160.13267950284209817No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAAACCTACT41760.13142068402955454No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA39020.12279777516363072No Hit
TTCTTACACTTTCCATGCATTCATTGTCACACTTGTGGTAGAACTCAAAA37790.11892690731505906No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT36950.11628338780871744No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA36510.11489868711491945No Hit
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT35640.11216075619763707No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC34360.10813253599749748No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT34030.10709401047714898No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG33730.10614989636774125No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA6550.0132.91231
GCAAAAG44400.0100.158783
AGCGAAA16350.084.750211
AGCAGGC3100.077.1944358
TACTAAT1750.074.587877
TAAACTA8100.073.4389955
GCAGGGG19750.073.072499
TGACGCC5000.072.490875145
GCAGGGT24300.071.029489
CTACTAA1800.068.489486
CTATTCG8700.067.52029
TTTTTAA9100.065.3728941
AAGGGTG14600.063.599695
ACAAGGG17550.063.2449533
GTCAATT5300.062.963182
AGGGTGT14900.062.7842756
ATTAGTA1850.062.7441831
CGTCGGG350.00356899662.13504145
CAAGGGT17950.061.8355944
AGCAAAA73600.061.110882
Produced by FastQC (version 0.11.5)