FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n02_SAMPLE_1_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n02_SAMPLE_1_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences720797
Sequences flagged as poor quality0
Sequence length151
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGAAAGCAGGGCCCGCTGGAGCCAGCAGAAGACTTAAGCCAGACATGC23230.32228214046395864No Hit
CTGCTAGATTGCCCTGTTGAATAATGCAGTTGGGGGAGTGTTCGTGTAGA20750.2878757819469282No Hit
AGCGAAAGCAGGGGCTTTTGGAGGAGGGTGCTTGTGTGTGTGTGTGTGTG19560.2713662792714176No Hit
ATGTATAATTGATGACTGCTAGATTGCCCTGTTGAATAATGCAGTTGGGG18960.26304216027536187No Hit
TATATGAACAGCTTGATAAACATGGTAGCCAAAAAAAAGTTTCCAAAGTT18500.2566603357117191No Hit
GTTTTGAGTTGGGTGGAGAGGTTGATCATCAGCGCCAAGGACAACAAGAA17180.23834727392039642No Hit
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT16190.22461247757690445No Hit
ATAAAAAGCCTTTTAACGAACTTCCTGAAAAGAAGAGCATATATGTTCAT15910.22072788871207843No Hit
AGCGAAAGCAGGTGGCACGTCACACCCCACCCCACAGCTCTTCAACTATA15360.21309744629902733No Hit
GCTTGATAAACATGGTAGCCAAAAAAAAGTTTCCAAAGTTTTAACACAGT15230.21129388718321523No Hit
GATAAACATGGTAGCCAAAAAAAAGTTTCCAAAGTTTTAACACAGTTTTG15170.21046147528360967No Hit
ACTCAAAACTGTGTTAAAACTTTGGAAACTTTTTTTTGGCTACCATGTTT14360.1992239146389344No Hit
GTCTACTGCATCATTTTGAGCTTTCTAAGGGAAAAAAACAAGACCACAGA14350.19908517932233347No Hit
GTTCATATAATAAAAAGCCTTTTAACGAACTTCCTGAAAAGAAGAGCATA13790.19131600159268142No Hit
GAATAATGCAGTTGGGGGAGTGTTCGTGTAGATTCTGCTTCTTGTTGTCC13690.18992864842667215No Hit
AGCGAAAGCAGGGGGTCTGGGGGGGGAAGTCCCATCATATTGCGAGGGCC13150.18243694133022195No Hit
TTATCAAGCTGTTCATATAATAAAAAGCCTTTTAACGAACTTCCTGAAAA12690.17605511676657923No Hit
GTTTGTATGTGTGTCAGTGCCGACTGAGCGTGTTGGCATGTTTGGCGTGT11790.16356893827249558No Hit
TATATGTTCATGGCACAGGGTTCCTGCTCGAGTGTCGAGTGCTTGTTTCC11770.16329146763929372No Hit
AGTAGAAACAAGGTAGTTTTTTACTCCAGCTCTATGCTGACAAAATGACC11610.16107170257367887No Hit
GTTGGGGGAGTGTTCGTGTAGATTCTGCTTCTTGTTGTCCTTGGCGCTGA11570.16051676130727513No Hit
GTTGAATAATGCAGTTGGGGGAGTGTTCGTGTAGATTCTGCTTCTTGTTG11250.1560772311760454No Hit
AGCGAAAGCAGGGAAGGGGGTTCGAATGCTGGGTGACTTCAATACCTGTG11150.1546898780100361No Hit
ATTTAGAGAAACTAGACGAAAACATGAAAACAATGAGTGTAACAGTATGC10750.14914046534599895No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT10750.14914046534599895No Hit
GCTTTTTATTATATGAACAGCTTGATAAACATGGTAGCCAAAAAAAAGTT10250.1422036995159525No Hit
TCCTAGCTCCAGTGCTGGTCTGAAAAATGATCTTCTTGAAAATTTGCAGG10190.1413712876163469No Hit
GGGAAGGGGGTTCGAATGCTGGGTGACTTCAATACCTGTGCCAGGCCACA10160.1409550816665441No Hit
AAGCAGGTGTGAGTGAGGCGCTAGTCATGCGTTGAGATGCCTATAAGCTA9980.1384578459677274No Hit
CACAGAGACACACTGAAACGTCAAGACACATCACGTCAACATCCACGGGA9670.1341570511530986No Hit
GGTCTGGGGGGGGAAGTCCCATCATATTGCGAGGGCCTCTGGATGCAGTG9650.13387958051989673No Hit
GGTGTGAGTGAGGCGCTAGTCATGCGTTGAGATGCCTATAAGCTAAACGC9590.13304716862029115No Hit
GTATAATTGATGACTGCTAGATTGCCCTGTTGAATAATGCAGTTGGGGGA9430.13082740355467629No Hit
GTTTTAACACAGTTTTGAGTTGGGTGGAGAGGTTGATCATCAGCGCCAAG9220.12791396190605678No Hit
GTATTGGTATTTTCTCTGTGAATTTGCATGTTAGGATGTTCCTACCTTCA9180.12735902063965304No Hit
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCTGCTGT8860.12291949050842331No Hit
TTCTAAGGGAAAAAAACAAGACCACAGACCAAGGATACAACTCACAGTGT8830.12250328455862053No Hit
CATATATGCTCTTCTTTTCAGGAAGTTCGTTAAAAGGCTTTTTATTATAT8770.12167087265901495No Hit
CTTATGACACCTGACATGAACTGAAACGGAGACATCCTGCCCCCAAATGT8600.11931237227679917No Hit
ATTTTATGTTGGTTTGCATTTTCCCGTGGATGTTGACGTGATGTGTCTTG8460.11737007784438615No Hit
GGCTTTTTATTATATGAACAGCTTGATAAACATGGTAGCCAAAAAAAAGT8370.11612145999497778No Hit
GATAGGAGCAGTATCTTTTCTCAGTGCACACAGAGACACACTGAAACGTC8360.11598272467837685No Hit
GTATGCAATATGAGGTACATGAAAAAACACACAGATTAAAGCATACTGTT8190.11362422429616105No Hit
TACCAATACTGACGCAATGTATGCAATATGAGGTACATGAAAAAACACAC8050.11168192986374804No Hit
AATCTGGACGGCCGGTTCACTGGTGAACAAAAGAAAACGACTGTGCATTT8040.11154319454714712No Hit
GTTCCTGCTCGAGTGTCGAGTGCTTGTTTCCTGTGCCGGATGTCCTTGTT7920.10987837074793597No Hit
GGTAGGAACATCCTAACATGCAAATTCACAGAGAAAATACCAATACTGAC7900.10960090011473411No Hit
ACATAAAATACACGCCAAACATGCCAACACGCTCAGTCGGCACTGACACA7890.10946216479813319No Hit
ATATGAGGTACATGAAAAAACACACAGATTAAAGCATACTGTTACACTCA7830.10862975289852761No Hit
TTCATATACCGGCGATTTTATTCAAGGTTGAAACCGGAAATGCACAGTCG7760.10765860568232109No Hit
ATACTATTTAGAGAAACTAGACGAAAACATGAAAACAATGAGTGTAACAG7750.10751987036572017No Hit
GTAGAAACAAGGTAGTTTTTTACTCCAGCTCTATGCTGACAAAATGACCA7630.10585504656650901No Hit
ACCTCATATTGCATACATTGCGTCAGTATTGGTATTTTCTCTGTGAATTT7560.10488389935030251No Hit
GAACATATATGCTCTTCTTTTCAGGAAGTTCGTTAAAAGGCTTTTTATTA7490.10391275213409601No Hit
GGTATGTGCAACCTGTGAACAGATTGCTGACTCCCAGCATCGGTCTCATA7450.1033578108676923No Hit
GCATATATGTTCATGGCACAGGGTTCCTGCTCGAGTGTCGAGTGCTTGTT7450.1033578108676923No Hit
TTGTTGTCCTTGGCGCTGATGATCAACCTCTCCACCCAACTCAAAACTGT7390.10252539896808671No Hit
ATCTAGCAGTCATCAATTATACATAGATGAAAGTGGAAGTAGGACGTTAA7380.10238666365148577No Hit
TCCTTGGTCTGTGGTCTTGTTTTTTTCCCTTAGAAAGCTCAAAATGATGC7340.10183172238508206No Hit
GGTGAGAGGTGTGAAACTAAAGCTGTGAGTAAGCAGAAACATTTGGGGGC7340.10183172238508206No Hit
GCAGTAGACTGACCATAAATACCACATATACTATTTAGAGAAACTAGACG7340.10183172238508206No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGGAA100.0070923404144.98709145
CCGTAGG100.0070923404144.98709145
AGCGAAA20150.0116.638131
GCGAAAG22850.0103.79392
CGAATAA4000.0103.30331145
CGAAAGC23700.0100.0713353
CAACGTA259.3889964E-486.992256145
GAAAGCA30300.079.470464
CAGGGGC4900.076.953289
TATTAGT300.001931413972.538831
CATCCAA300.001936205472.49355145
TAGTAGA1500.067.693512
AAAGCAG40200.064.0490345
CATCAGG350.003560498862.1675152
AGCAGGT8950.058.3350227
AGCAGGC5550.057.4883167
GCAGGGG12050.057.1684578
AGGCGTG1051.1041266E-955.2600145
AGCAAAA2850.053.4496731
CAGGCGT1101.6680133E-952.7481964
Produced by FastQC (version 0.11.5)