FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n02_PR8_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n02_PR8_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1812665
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT61050.3367969260729368No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA51850.2860429257474492No Hit
GTTAAGGACAGAAGCCCTTATAGGGCCTTAATGAGCTGCCCTGTCGGTGA37800.2085327404677643No Hit
GTATATAGCCCACCCACGGATGGGACAAAGAGATGAATTGCCGGTTAATA36780.20290566651863418No Hit
ATATACAGCAAAGACAATAGCATAAGAATTGGTTCCAAAGGAGACGTTTT35880.1979406012694017No Hit
GTCCTTAACAGTCCCATTTGAATGCTTGTCATTCAGTAAGGCACCTTGGG35800.19749926213613656No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT33730.18607961206290186No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG32680.1802870359387973No Hit
GTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCGCTATACCCTG32130.17725282939759968No Hit
GTATTAAAATACAACGGCATAATAACTGAAACCATAAAAAGTTGGAGGAA28640.15799940970890924No Hit
GGTATGGTAATGGTGTTTGGATAGGAAGGACCAAAAGTCACAGTTCCAGA28390.1566202249174558No Hit
TTTTTGAACAGACTACTTGTCAATGCTGAATGGCAACTCAGCACCGTCTG27840.15358601837625815No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA27710.15286884228470235No Hit
GCCACTGCTCCATTATCTGGACCTGAAATTCCGATTGTTAGCCAGCCCAT26290.14503507266924667No Hit
CAATATGGATTAGCCATTCAATTCAAACTGGAAGTCAAAACCATACTGGA26200.14453856614432342No Hit
GGATTAGCCATTCAATTCAAACTGGAAGTCAAAACCATACTGGAATATGC25990.14338005091950248No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT25170.13885632480353513No Hit
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT24240.13372575737932824No Hit
TACCATACCTATATGAAAATCCCTTTACTCCGTTTGCTCCATCAACATAC23910.13190523345460967No Hit
GCTGTATATAGCCCACCCACGGATGGGACAAAGAGATGAATTGCCGGTTA22640.12489897471402603No Hit
GGGTAAAGGACACAACTTCAGTGATATTAACCGGCAATTCATCTCTTTGT21690.11965807250650284No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAAACCTACT21450.11833405510670751No Hit
GACTACTTGTCAATGCTGAATGGCAACTCAGCACCGTCTGGCCAAGACCA21360.11783754858178427No Hit
GTGTAAATGGTTCATGTTTTACTATAATGACTGATGGCCCGAGTGATGGG20950.11557568552380058No Hit
CTATTGATTTAGTAACCTTCCCCTTTTCGATCTTGAAAATTTTGTACGAG20090.11083128984120066No Hit
GCCTCATACAGTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCG19970.11016928114130299No Hit
ATATAGCCCACCCACGGATGGGACAAAGAGATGAATTGCCGGTTAATATC19940.11000377896632858No Hit
GCCTAATATTGCAAATAGGGAATATAATCTCAATATGGATTAGCCATTCA18920.10437670501719844No Hit
GGTTTAAAATGAATCCAAATCAGAAAATAACAACCATTGGATCAATCTGT18760.10349402675066822No Hit
GCATCCACAGCACTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTT18370.10134249847600081No Hit
GGTATGTGCAACCTGTGAACAGATTGCTGACTCCCAGCATCGGTCTCATA18340.10117699630102638No Hit
ATCAAATAGGATACATCTGCAGTGGGGTTTTCGGTGACAACCCGCGTCCC18240.100625322384445No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTGCA100.0070773815145.095555
GAGCGAA5550.0138.563541
TCGGTTT256.469967E-6115.98678145
GCAAAAG29900.096.0886463
TGACGCC3200.088.349304145
GCAGGGT12850.083.520079
AGCAGGC2050.074.282388
GCAGGGG18350.073.503699
AGCAAAA40100.073.0925452
ACGGCCC1100.072.49174145
TACGTAA300.001936872572.49174145
AGCGAAA11100.069.281771
AGGGTGT6000.068.889956
AAGGGTG6100.067.790545
AGCAGGG36000.067.276648
ACAAGGG7500.065.780283
CAAGGGT7600.064.914764
CTGCCGG902.8194336E-1064.4371145
AAGCAGG57450.063.3627477
CGTGGGG350.00356858262.135777145
Produced by FastQC (version 0.11.5)