FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n02_POSITIVE_FLUB_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n02_POSITIVE_FLUB_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3339173
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT76880.23023664841564065No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGGCAACAAGC71580.21436445491144063No Hit
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGTACTTCCTT49720.1488991435903441No Hit
GTAATATTCAGTGAATCAAAGGTGGGGAGAGAAAATTCTCCTGCATCAAA43660.13075093743271163No Hit
TTATAGATATTTCTTCACAAGAGCTGAATTTCCCATGGAGCTCTGCTTTA43480.1302118818042671No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACCA43210.1294032983616003No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAA41680.12482132551982182No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT41320.12374321426293278No Hit
GTATAAACACTGGGACAATTGACAAACCACCGGAAGAAATAACTTCTGGA39840.11931097909572219No Hit
CTTATAGACAGATGGAGCAAGAAACATTGTCTCTGGAGACCATATAAACA37000.11080587918026409No Hit
GTATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT35820.10727207006046109No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACGC10500.0114.020371
CACGCAC10900.0111.165823
GCACGCA10900.0110.49852
GTAACAA31750.0103.294214
CGGTGCA1300.0100.4624942
CACGATG259.394406E-486.98666145
GAAACAC11550.086.063684
GAGCAGA3050.085.642721
AAACACG12000.082.830085
GCTGCCC3400.081.01698145
ACGTGCA6550.080.864212
AACACGT3300.079.116696
CAACGCA7800.075.3139
TAACAAG43850.075.116455
TAGTAAC44600.074.9964752
CGCACTT17950.070.328865
ACGCACT17900.070.1252444
TGACGCC4750.068.673676145
TCTGACC3150.066.735794145
ACACGTG3950.066.095517
Produced by FastQC (version 0.11.5)