FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n02_PLASMID_CONTROL_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n02_PLASMID_CONTROL_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2433611
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATACATCATGGTCATAAGTTCCATTTCTGATTGACCCTATGCAGGCAT129160.5307339587140262No Hit
GATGTATACAGAGATGAAGCATTAAACAACCGGTTCCAGATCAAAGGTGT88530.36378040697547803No Hit
GTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAG71360.2932268139813635No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTCCAAAATAA65870.2706677443519116No Hit
ATCTGGGACAGGCCCCATATGTGATCCTGTTTACATTTTGAAATGGTTTG61560.25295743650073904No Hit
GTATTATCCCGTGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACA58250.23935624880065057No Hit
TCTAATAACAGTTTCTTTAGTAGATTGAATTGGTTGACCCACTTAAAATT54490.2239059570325742No Hit
ATTGTATGTTGGTTCTCCAGGGCAACAAGAAGCTCCGCGTTGTATGACCA53850.22127612013588038No Hit
CCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTT52270.21478371029716747No Hit
ACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAAT52120.21416734227450485No Hit
GTTATTAGATCTCCTTTTGCAAGCAGAGCTTGTTCCATTTTGAGTGACTC49370.2028672618590235No Hit
GGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAA48290.19842941209585263No Hit
CTAAAATAGATCTCTGGTCATACAACGCGGAGCTTCTTGTTGCCCTGGAG47840.19658030802786478No Hit
TCATAAGGGTAACAGTTGCTGTGGGCTTTGCTGCGTTCAACAAAAAGGTC46430.19078644861483612No Hit
CCCTTACCCTGGGTCTAGATCCGATATTCGGGATTACAGTTTGTTGGCTT44550.18306130273079796No Hit
CTATTGGACAATAGTAAAACCGGGAGACATACTTTTGATTAACAGCACAG43930.18051364823712582No Hit
CATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATC43180.1774318081238127No Hit
CATATGGGGCCTGTCCCAGATATGTTAAGCAAAACACTCTGAAATTGGCA43150.1773085345192802No Hit
ATTTTAGTGTCCTCAACATATTTCTCGAGGTCCTGAATTCTCCCTTCTAC43090.17706198731021516No Hit
GTCCAATAGATGCTTATTCTGCTGGGGATATCCCTTACCCTGGGTCTAGA42950.17648671048906336No Hit
CTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCT40340.1657619068947338No Hit
ACATACAATTGATCTAACTGACTCAGAAATGAACAAACTGTTTGAAAGAA39610.16276224918444238No Hit
TCTCAAAAGCACTCAAGCAGCAATCAACCAAATCAATGGGAAGCTGAATA38040.1563109305472403No Hit
TAGTAAAACCGGGAGACATACTTTTGATTAACAGCACAGGGAATCTAATT37920.1558178361291102No Hit
GTCCCAGATATGTTAAGCAAAACACTCTGAAATTGGCAACAGGGATGCGA37260.15310581682939467No Hit
TCTCTGTATACATCATGGTCATAAGTTCCATTTCTGATTGACCCTATGCA36780.1511334391568743No Hit
GTCCGTACCCGGGTGGTGAACTCCCCAAATGTACAATTTGTCAAAATTTT36400.14957197349946233No Hit
CTATTTTAGTGTCCTCAACATATTTCTCGAGGTCCTGAATTCTCCCTTCT36400.14957197349946233No Hit
CTGTATACATCATGGTCATAAGTTCCATTTCTGATTGACCCTATGCAGGC36380.14948979109644064No Hit
GCATTAATGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGC35850.14731195741636605No Hit
CTATTGGGAGACCCTCAGTGTGATGGCTTCCAAAATAAGAAATGGGACCT35530.14599703896801913No Hit
ACATTCTATGTCTGGTTTTCGCTCAAAAACTTCCCGGAAATGACAACAGC35410.14550394454988905No Hit
GAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGG35260.1448875765272264No Hit
TCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATAC34680.14250428683959762No Hit
ATGCAGGCATTGTCACATTTGTGGTATATTTTGAAACAACCATTGCCCAT34290.1409017299806748No Hit
GATGAGGACTGTCGCATATTCCACCTGTTGAGGAACTCTGAACCAGCTCA33550.13786098106887257No Hit
CTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGT33470.13753225145678583No Hit
GTTTTACTATTGTCCAATAGATGCTTATTCTGCTGGGGATATCCCTTACC33270.136710427426569No Hit
ATCTATTAGTGTGCAGTTTTCTCCATCAAGGATCTGATGAGGACTGTCGC32790.1347380497540486No Hit
CCCATATCCTCAGCATTTTCCCTCAGTTGCTTCTTTGTTCTTTCAAACAG32700.13436822894045106No Hit
GTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAATGTTGCCTTT32160.1321493040588656No Hit
GTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACT31770.1305467471999428No Hit
ATACAGAGATGAAGCATTAAACAACCGGTTCCAGATCAAAGGTGTTGAGC31430.1291496463485742No Hit
GGTACGGACAATGACCAAATCAGCCTATATGCTCAAGCATCAGGAAGAAT30680.1260678062352611No Hit
GTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCT30580.1256568942201527No Hit
ATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAAT30350.12471179658540334No Hit
ACCCTATGCAGGCATTGTCACATTTGTGGTATATTTTGAAACAACCATTG29900.12286269251741548No Hit
TTCTTTAGTAGATTGAATTGGTTGACCCACTTAAAATTCAAATACCCAGC29870.12273941891288295No Hit
TGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATC29430.12093140604640593No Hit
GTCCTGGGGTTGACCAGAGGGCCCCGGGCGCTCCGTGTGTGGCTGCGATG28800.11834266035122293No Hit
ATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTG28590.11747974511949527No Hit
CCCTTATGATGTGCCGGATTATGCCTCCCTTAGGTCACTAGTTGCCTCAT28590.11747974511949527No Hit
TTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGT28550.11731538031345191No Hit
ACTAAAGAAACTGTTATTAGATCTCCTTTTGCAAGCAGAGCTTGTTCCAT28130.11558954984999656No Hit
GATCTAGACCCAGGGTAAGGGATATCCCCAGCAGAATAAGCATCTATTGG28030.11517863783488816No Hit
ATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGC27700.11382262818503039No Hit
GGTATATCTTTCGCTCCGAGTCGGCATTTTGGGCCGCCGGGTTATTAGTA27290.1121378889230859No Hit
CTATTAACCATGAAGACTATCTTTGCTTTGAGCTACATTCTATGTCTGGT27210.11180915931099918No Hit
ATGTGATCCTGTTTACATTTTGAAATGGTTTGTCATTGGGAATGCTTCCA26970.11082297047473899No Hit
GGGTAAGGGATATCCCCAGCAGAATAAGCATCTATTGGACAATAGTAAAA26780.11004223764603299No Hit
TACTAATGCTACTGAGCTGGTTCAGAGTTCCTCAACAGGTGGAATATGCG26660.1095491432279029No Hit
TTCTATGTCTGGTTTTCGCTCAAAAACTTCCCGGAAATGACAACAGCACG26660.1095491432279029No Hit
GCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAG26570.10917932241430532No Hit
CAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCG26540.1090560488097728No Hit
CACTAAAATAGATCTCTGGTCATACAACGCGGAGCTTCTTGTTGCCCTGG26400.10848077198862102No Hit
ACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATA26380.10839858958559934No Hit
AACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAG26240.10782331276444755No Hit
ATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTT26220.10774113036142588No Hit
ATTGGGAGACCCTCAGTGTGATGGCTTCCAAAATAAGAAATGGGACCTTT26170.10753567435387167No Hit
GTATACAGAGATGAAGCATTAAACAACCGGTTCCAGATCAAAGGTGTTGA26130.10737130954782831No Hit
GTCCTATTCCCTCAGAATTTTGATGCCTGAAACCGTACCAACCATCCACC25990.10679603272667652No Hit
TTTGGGGAGTTCACCACCCGGGTACGGACAATGACCAAATCAGCCTATAT25880.10634402951005728No Hit
GTCATTGGGAATGCTTCCATTTGGAGTGATGCATTCAGAATTGCATTTGC25850.10622075590552475No Hit
GTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATA25600.10519347586775372No Hit
CTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGA25330.10408401342696101No Hit
GTCCTGAATTCTCCCTTCTACTTCTGAGAATTCTTTTTCAATCTGATGGA25310.10400183102393931No Hit
GAATAGGACAAGCAGCAGATCTCAAAAGCACTCAAGCAGCAATCAACCAA25250.10375528381487427No Hit
TATGGGGCCTGTCCCAGATATGTTAAGCAAAACACTCTGAAATTGGCAAC25170.10342655420278754No Hit
ATTAGATTCCCTGTGCTGTTAATCAAAAGTATGTCTCCCGGTTTTACTAT24910.10235818296350568No Hit
GTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCAT24880.10223490935897316No Hit
GATCTAACTGACTCAGAAATGAACAAACTGTTTGAAAGAACAAAGAAGCA24780.10182399734386474No Hit
TTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCC24770.10178290614235389No Hit
TTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATT24610.10112544691818044No Hit
GGATATGGGCAATGGTTGTTTCAAAATATACCACAAATGTGACAATGCCT24610.10112544691818044No Hit
CTACCAAAAGAAGCCAACAAACTGTAATCCCGAATATCGGATCTAGACCC24410.1003036228879636No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGGAC3550.071.5340353
TTTCGGA3600.070.5405042
TCGGACA4000.063.486454
CTTTCGG4050.062.7039571
CGGACAT4050.062.701385
CTTCCCC2150.060.688347145
GGACATC4350.058.349556
GCTGCCC3650.057.59389145
AAGCCCC2850.055.956318145
AGAGTGG2400.054.4158445
GTACCCC953.1457603E-853.41284145
TGCAGCC2450.053.257126145
TCTGACC3200.052.10137145
GACATCT5000.050.764117
ACCCGAA604.9502373E-448.32591145
CCTTTCC2200.046.129276145
GAGTGGC2850.045.8022046
TACACAA2700.045.641136145
ATAGGGG803.7396327E-545.305542145
GTCCAGA2900.045.0356521
Produced by FastQC (version 0.11.5)