FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n02_NEGATIVE_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n02_NEGATIVE_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9588
Sequences flagged as poor quality0
Sequence length151
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTCTGCTTCTGCTTCTGCTTCTGCTTCTGCTTCTGCTTCTGCTTCTGC520.5423445974134334No Hit
AGCAGAAGCAGAAGCAGAAGCAGAAGCAGAAGCAGAAGCAGAAGCAGAAG360.37546933667083854No Hit
GTAGTAACAAGAGCCGGGTTATTAGTAGTAACAAGAGCCGGGTTATTAGT150.15644555694618273No Hit
GTAGTAACAAGAGGATTGGGGGGAGCAGAAGCAGAGGATTGGGGGGAGCA150.15644555694618273No Hit
TCCCCACACCCACCAAAGTCATGAGGCGATCATCAAGCGCCTCAAACGAG140.14601585314977056No Hit
GTAGTGGGCGGTGCGATGGCGCAGTGAATGCGACAACGCCACTCATTGAG130.13558614935335836No Hit
GTGTGGGGATGGCTGTGTTCGTGTTCGTGCTCACTCATGACTTGAGCCTC120.1251564455569462No Hit
GCAGAAGCAGAAGCAGAAGCAGAAGCAGAAGCAGAAGCAGAAGCAGAAGC110.114726741760534No Hit
CTAATAACCCGGGCTCTTGTTACTACTAATAACCCGGCTCTTGTTACTAC110.114726741760534No Hit
GTAGTAACAAGAGCCGGGTTATTAGTAGTAACAAGAGCCCGGGTTATTAG110.114726741760534No Hit
CTGCTTCTGCTTCTGCTTCTGCTTCTGCTTCTGCTTCTGCTTCTGCTTCT110.114726741760534No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTGTG100.0068764617145.736852
ATATGGC100.0068764617145.736852
AATATGG100.0068764617145.736851
TATGGCG100.0068764617145.736853
CGAGAGC100.0069858143144.973828
ACGTGCC202.025119E-6144.973829
ACACGTG301.520715E-596.6492167
CACGTGC301.520715E-596.6492168
CCAAAGC258.919661E-487.442113
AACACGT353.268532E-582.8421866
AAGAGCG350.003461803562.1316389
AAACACG901.7060302E-856.6754385
GAAACAC1003.9173756E-851.0078934
CAGAGGA750.001375857638.863164
CAAGAGC1156.4375217E-637.819268
AACAAGA1208.621466E-636.2434546
ACAAGAG1208.621466E-636.2434547
TAGTAAC1301.4388448E-533.6315772
GTAGTAA1351.8631135E-532.3859631
TAGAAAC1601.5687092E-631.8799342
Produced by FastQC (version 0.11.5)