FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n02_CM554145_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n02_CM554145_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences921027
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA3600.072.5381
GCGAAAG6300.067.932422
GAGCAAA19000.065.665981
AGCGAAA6650.064.3570251
CGAAAGC6800.062.944223
CAAAAGC23600.054.1020164
GCAAAAG24100.051.474473
AGCAAAA25450.049.023722
GGCGCGA450.00961896248.3586651
GCCGACC2700.045.64232145
AAAAGCA27850.045.5829125
AAAGCAG36000.042.304646
TGACGCC2800.041.423286145
ACATCGA750.001481486338.676438
AAGCAGG39650.037.8594937
ATATCGT3350.036.810331
TCTCGGG5950.036.549957145
TCGTGTA3200.036.2729454
ACGTGTT3300.035.147026145
ATCGTGT3350.034.648783
Produced by FastQC (version 0.11.5)