FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n02_CM554007_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n02_CM554007_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2263414
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA43160.19068539825237452No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT32320.14279314345497554No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA31230.137977409347119No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG30420.1343987445513724No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT30070.13285240791123498No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC29720.13130607127109756No Hit
TCCTTATTCTGGGTCTAGATCCGATATTCGGGATTACAGCTTGTTGGCTT26870.11871447291569284No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG25680.1134569283392256No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT24350.10758084910670342No Hit
ATTATATTCAGTATGGAGAGAATAAAAGAACTACGGAATCTGATGTCGCA22920.10126295940557052No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTTA1350.069.8529054
ACGACGG754.838512E-967.703594
TAAACTA4550.063.769635
ATTACGA808.052666E-963.4777261
TACGACG808.058123E-963.472113
TGACGCC5550.062.69672145
CGAAAGC13500.061.7929573
TACTAAT2150.060.709327
CGACGGG851.3009412E-859.7371445
GAGCGAA10300.057.0507161
GCAAAAG52500.055.6827473
AGCGAAA15550.053.184831
GTCGCCC4000.052.557487145
CACGTTT1700.051.20443
ATTAGTA2000.050.782181
AAGCAGG70200.050.7184227
GCCACGT1750.049.745811
AGCAAAA61550.048.4382742
CGTTTAT1950.048.3586435
CCGCCGG450.009644330548.328724145
Produced by FastQC (version 0.11.5)