FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n02_CM553981_2_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n02_CM553981_2_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2069956
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACCATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA46980.22696134603827325No Hit
ATATCGTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCC37750.1823710262440361No Hit
GAGCAAAAGCAGGTGGGGAGGTAAGAGAGGAGTAGGGGAGGGCCAGAGAC33670.16266046234799195No Hit
ATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCAAGGCA27550.1330946165039257No Hit
TCCCACCTGCAGTCTGGATTCTGTCCCAATCACACACTAAAGAAGAGCCC25240.12193495900395951No Hit
GAGCAAAAGCAGGACAGGCAAGGCTGGCAAGAAGGAACCAGTTAAGAGGC25090.12121030591954612No Hit
CTAGAAAAAGAAGGGGCAGCCTCTGGGAAGGGAGGCAAAGGCAGCCAGGT24780.11971268954509177No Hit
GTTAAGATACTTGTGGATAGTCGCACAGCTAGAAGATAACAGAGCTAGGA23080.11149995458840671No Hit
GGACCAATACATGGGTACCACACTGGGTGCTGAGACTTGGCCTTCTTCAC22220.10734527690443661No Hit
AAACATGGCTACAGTCATGTTAAGGGCAGAGGCTCACTGGGGTAAGTGGC20940.10116157058410905No Hit
GAACCAGTTAAGAGGCTGTTTTTGATCTGGGACAGAGAGAGGGTGATGAC20930.10111326037848148No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA38500.0103.824731
GAGCGAA9600.095.215861
AGCAAAA54150.073.146512
AAAGCAG74400.066.276116
AGCGAAA15800.065.658191
CAAAAGC60550.065.654714
GCAAAAG61550.064.9416053
GCGAAAG16000.064.83592
AAAAGCA63950.062.8416635
CGAAAGC16750.061.9327933
TACGACG1900.057.271313
ACGACGG1900.057.271314
TGACGCC5100.054.01427145
CGACGGG2200.049.4591945
ATATCGT18100.049.2988171
ACGATCC5000.049.295128145
ATCGTGT18350.048.625993
TATCGTG18600.047.972422
CATACCG3200.047.589529
ATTACGA2300.047.3122251
Produced by FastQC (version 0.11.5)