FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n02_CM553980_3_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n02_CM553980_3_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4110834
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA89770.21837417905952905No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG60790.14787753531278566No Hit
TCCTTATTCTGGGTCTAGATCCGATATTCGGGATTACAGCTTGTTGGCTT60370.14685584482370242No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC58790.1430123425076274No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA58540.14240419340698263No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT57930.14092030960140936No Hit
GTATATACATTGAAGTTTTACATTTGACTCAAGGAACGTGTTGGGAACAA57500.13987429314830033No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT57370.13955805561596502No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG50250.12223796922960159No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG46620.11340764428823932No Hit
ATTATATTCAGTATGGAGAGAATAAAAGAACTACGGAATCTGATGTCGCA45700.11116965559786651No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT45430.11051285456917014No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC45070.10963711986424166No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC44190.10749643502997203No Hit
TTCTTAGACAGAACCCGACAGAAGAACAAGCTGTGGATATATGCAAGGCT42840.1042124298864902No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG42260.10280152397299429No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT42150.10253393836871058No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATAA10500.090.44104145
GCTGACC2450.088.76437145
GCTGCCC3300.081.27768145
TGACGCC4850.073.23824145
GCAGGGG10500.073.211589
ATTAGTA2600.072.550041
GCAGGGT17400.066.685889
AGGGTGT11700.063.222586
TCACGCC1300.061.33846145
GTCGACC502.0122301E-457.992725145
CGAAAGC22850.056.5160033
CGCATGC400.006055223854.36818145
GTAACGA400.006055223854.36818145
TAAACTA8100.052.843165
TTTAAAC8200.052.2006383
CTTATAA3000.050.743637145
AAGCAGG115800.049.6612057
GCAAAAG98950.048.977693
TACATTG22000.048.7861986
ACGGTAG450.00964603348.32727145
Produced by FastQC (version 0.11.5)