FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n02_CM553980_2_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n02_CM553980_2_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2503845
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA55470.22153927259874312No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG39100.1561598261873239No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT37750.15076811863354161No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT37290.1489309442078084No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA36390.14533647250528686No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC36310.1450169639095072No Hit
TCCTTATTCTGGGTCTAGATCCGATATTCGGGATTACAGCTTGTTGGCTT34100.13619053895109323No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG31390.12536718526905619No Hit
GTATATACATTGAAGTTTTACATTTGACTCAAGGAACGTGTTGGGAACAA30810.12305074794965343No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT29280.11694014605536683No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG27400.10943169405454412No Hit
ATTATATTCAGTATGGAGAGAATAAAAGAACTACGGAATCTGATGTCGCA26880.10735488818197612No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC26820.10711525673514136No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT26250.10483875799021106No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC25780.10296164499000537No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCCC259.392416E-486.99065145
GCTGCCC4600.080.371796145
GCTGACC1100.072.49221145
AGACGCC608.042662E-872.49221145
CCGACCC300.001936908972.49221145
GCAGGGG5750.071.889129
GCAGGGT10950.065.565789
GTCGCCC3400.061.831593145
TAAACTA4450.060.3165865
CGAAAGC13150.058.4783
TTTAAAC4750.056.5093733
CCTCGCC651.0948188E-555.76324145
GCAAAAG56600.054.7297973
CAGTAGG400.006054516454.36916145
AAGCAGG69100.052.7840547
AGCGTAA553.2216476E-452.721607145
CAGGGGG553.2216476E-452.721607145
TGACGCC4300.052.261826145
TATATTC8750.051.40393
AGGGTGT7650.050.2414256
Produced by FastQC (version 0.11.5)