FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n02_CM553698_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n02_CM553698_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1456276
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACCATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA24110.1655592758515556No Hit
ATATCGTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCC23940.16439191471946252No Hit
GAGCAAAAGCAGGTGGGGAGGTAAGAGAGGAGTAGGGGAGGGCCAGAGAC19660.13500188151147172No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA6200.078.3936541
CAAAAGC37700.066.575994
GAGCAAA38750.066.271891
GCAAAAG38300.065.154223
CGAAAGC10600.063.6443673
AGCGAAA10500.062.8709341
TGACGCC3100.060.798965145
GCGAAAG11600.058.783132
AGCAAAA44200.057.113642
TACGAAC400.00603795554.4056663
AAAGCAG57400.051.4198846
ATTACGA1501.0913936E-1148.362251
ACGACGG1501.0913936E-1148.360594
CGACGGG1501.0913936E-1148.3589325
TACGACG1551.4551915E-1146.8005753
AAGCAGG65800.044.8541037
CCGACGC1851.8189894E-1243.102806145
AAAAGCA60750.041.911075
ACGTACA700.001053872441.4519352
ATATCGT10950.041.074791
Produced by FastQC (version 0.11.5)