FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n02_CM553445_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n02_CM553445_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1933996
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC15200.068.7292563
AGCGAAA15350.067.588511
GCGAAAG16500.062.8745422
GAGCGAA9250.059.609721
GAGCAAA38450.057.7391361
GCAAAAG38200.057.1644333
TGACGCC5450.054.533314145
AGCAAAA40600.053.7852482
CGTACTC2900.050.032793
TCGTACT2900.050.032792
CAAAAGC49300.046.353914
GTCGCCC5200.043.214832145
TCGCCGG14900.038.43398145
AAAGCAG72500.038.1092076
TCTCGGG11000.037.562687145
AAGCAGG73550.037.4665687
TCGCGGG800.002038547336.244698145
AAAAGCA68950.033.8791925
CGCCGTT3050.033.273823145
TACGACG1801.2860437E-732.243353
Produced by FastQC (version 0.11.5)