FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n02_CM552690_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n02_CM552690_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1908070
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACCATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA29990.15717452713998964No Hit
ATATCGTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCC28330.14847463667475513No Hit
GAGCAAAAGCAGGTGGGGAGGTAAGAGAGGAGTAGGGGAGGGCCAGAGAC25710.13474348425372235No Hit
GAGCAAAAGCAGGACAGGCAAGGCTGGCAAGAAGGAACCAGTTAAGAGGC20250.1061281818801197No Hit
ATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCAAGGCA19250.10088728400949651No Hit
GTTAAGATACTTGTGGATAGTCGCACAGCTAGAAGATAACAGAGCTAGGA19230.10078246605208403No Hit
ATCTTGGACAGAGAGAGGGTGATGACTGATCTGGGGTTGGAGAAGAAAGC19130.10025837626502174No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA9600.082.398841
GAGCAAA38500.078.603371
TGACGCC3800.064.85578145
AGCAAAA46500.064.764672
AGCGAAA13700.060.917651
GCGAAAG14400.058.457272
CAAAAGC52350.057.6674964
GCAAAAG52750.056.9550633
CGAAAGC15000.056.1204573
ATCGACC400.00605639154.364407145
CGACGGG2100.051.8313145
AAAGCAG73200.051.2278446
CGTGTAG13300.048.0121655
ATATCGT13750.047.5013161
GACGAAA9050.046.455032145
TACGACG2350.046.320993
AAAAGCA65900.046.0268365
TCGTGTA13900.045.9433144
ATCGTGT13950.045.778643
TATCGTG13950.045.7774432
Produced by FastQC (version 0.11.5)