FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n02_CM552654_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n02_CM552654_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3049928
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCGTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCC36860.12085531199425037No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA12600.070.256081
TGACGCC6050.065.89942145
GAGCAAA64850.061.3147851
CGACGAA8200.057.461086145
GCAAAAG70150.056.3701483
CGAAAGC19700.056.351513
AGCAAAA70750.056.097212
AGCGAAA20000.055.1452641
CAAAAGC75500.052.8562164
GCGAAAG21900.051.0219352
GGTCGTT1053.818497E-641.441527
AAAGCAG119150.040.415486
TAGAAAC19950.038.9153752
ACGATAA7700.038.598236145
AAGCAGG128850.037.372957
AAAAGCA111900.036.698855
CGTGTAG19800.034.4452065
GTCGCCC8550.033.913155145
TATCGTG20400.033.788882
ACGTTTA7950.033.768754
Produced by FastQC (version 0.11.5)