FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n02_CM552549_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n02_CM552549_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2173201
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG144800.6662982393253086No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTGATGTCGCA142400.6552546220989223No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC116580.5364437067717159No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC92370.4250412180005439No Hit
TTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCG90710.4174027160856267No Hit
ATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT66370.30540203138135863No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT58590.2696023055391563No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC56820.2614576378346964No Hit
GTATGGAAAGAATAAAAGAACTACGGAATCTGATGTCGCAGTCTCGCACT53940.24820529716303277No Hit
TAATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGG50520.23246814261543228No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG50060.23035144931370818No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT49650.2284648313708672No Hit
GAATAAAAGAACTACGGAATCTGATGTCGCAGTCTCGCACTCGCGAGATA47470.2184335457235663No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTGATGGCCATCC44470.2046290241905834No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA42540.1957481153376977No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC42460.19537999476348483No Hit
TCAATGAACTGAGTAACCTTGCAAAAGGGGAAAAGGCTAATGTGCTAATC40370.1857628447621734No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA40240.1851646488290775No Hit
TCTTAATTATGGCCATATGGTCCACTGTGGTTTTTGTCAGTATCTCGCGA40220.18507261868552427No Hit
GCATTAAGCATCAATGAACTGAGTAACCTTGCAAAAGGGGAAAAGGCTAA39800.18313998567090667No Hit
AGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACG39030.17959682514410769No Hit
ATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGGAT38320.1763297550479684No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCCATAACTATTTTAA37960.17467321246401046No Hit
TTCTTAATCTGTGTCTCACTTCTTCAATTAGCCATCTTATCTCTTCAAAC35920.16528613782158208No Hit
GTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTTTT35060.16132884164879363No Hit
GAGTAACCTTGCAAAAGGGGAAAAGGCTAATGTGCTAATCGGGCAAGGAG34380.1581998167679842No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA33810.15557695767671745No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT33410.15373635480565304No Hit
AAACAAGGTCGTTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAAT33150.1525399629394612No Hit
ATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGCCC32220.14826056126423648No Hit
TAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATC31990.14720221461337446No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG31520.1450395062398738No Hit
CTAGTATACTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGGCC30920.14227860193327724No Hit
AGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTCAACCC30910.14223258686150062No Hit
GAAATATCCAATCACTGCTGACAAAAGATAGGTAAGGAAGACAGAAGATA29460.1355604014538922No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA29390.13523829595145595No Hit
GAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC29220.13445603973125358No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA28150.12953242705115633No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTTCTTA27570.126863552888113No Hit
GCTTAATGCTGGTCCGTATCTTCTGTCTTCCTTACCTATCTTTTGTCAGC26980.12414866365329301No Hit
GGATATTTCATTGCCATCATCCATTTCATCCTAAGTGACGGGTTCTTTTC26950.12401061843796317No Hit
GGACAAGCTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTAGAA26040.11982324690629167No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT25750.11848880982476999No Hit
GCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACGG25550.11756850838923781No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC25210.1160039959488331No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG25110.11554384523106698No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAA24960.11485361915441784No Hit
ATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCATTGAGCAAA24880.11448549858020496No Hit
GTACGGATAACAAATAGTAGCACTGCCATAACTATTTTAATGCATGTGTC24680.11356519714467277No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT24430.11241482035025752No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT24360.11209271484782127No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT24240.11154053398650195No Hit
AGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTGATGGCCATC23990.1103901571920867No Hit
GTTCATTGATGCTTAATGCTGGTCCGTATCTTCTGTCTTCCTTACCTATC23860.10979196125899078No Hit
GGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTGATG23170.1066169213064047No Hit
CAATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTGATGTCG23120.10638684594752165No Hit
GTGGTAGCCTGTATGCATCACCACAATTGGAAGGATTTTCAGCGGAGTCA22980.10574263494264911No Hit
AAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTC22660.1042701526457976No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTACC22000.10123315790854137No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT21790.10026684140123257No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTAA203.8690938E-4108.7329145
GTCTAGT259.360665E-487.064441
TGACGCC3950.078.91164145
GCAGGGG5700.077.607669
CAAAAGC73650.076.339364
CACGTGG300.001937257472.4886145
GTCGCCC3100.070.15025145
CTTATAA1700.068.22456145
GCAGGGT15800.066.55199
GCAGGTC36400.066.5418249
CAAGCTA7500.064.808674
CGAAAGC18300.063.825523
AAGCAGG102500.063.6012237
GAGCGAA15150.063.215111
AGCAGGG23700.062.7241978
TACAGCG350.003569289362.133087145
AGCAAAA92250.061.2619322
GAGCAAA93850.060.454981
CGACGAA12950.060.453808145
TACTCCC8400.060.40716145
Produced by FastQC (version 0.11.5)