FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n02_CM552492_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n02_CM552492_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2538099
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC32330.12737879806894845No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT31340.12347824099847957No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT27260.10740321792018356No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC26560.1046452482743975No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA25710.10129628513308583No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG25700.10125688556671747No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA10900.068.55621
CGAAAGC18100.064.129863
AGCGAAA18000.064.0856251
TTACGGG350.003569136462.13406145
GCAAAAG56850.061.891383
TGACGCC5950.059.69743145
GAGCAAA63750.056.4465681
CAAAAGC64000.056.2238544
AGCAAAA63100.055.9910352
GCGAAAG21050.054.79792
CGACGAA14400.052.857098145
CGACGGG1600.049.8765
CGCGAAA450.00964622748.32649145
ACGACGG1751.8189894E-1245.6009144
TATCGTG11650.044.8358572
ATATCGT11550.044.5976941
TACGACG1903.6379788E-1242.000843
AAAAGCA88450.041.9123465
CGTGTAG13050.040.025885
TTACGAC2005.456968E-1239.90082
Produced by FastQC (version 0.11.5)