FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n02_CM551963_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n02_CM551963_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2199580
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACCATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA30310.1377990343611053No Hit
ATATCGTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCC26130.11879540639576647No Hit
GAGCAAAAGCAGGTGGGGAGGTAAGAGAGGAGTAGGGGAGGGCCAGAGAC24140.10974822466107165No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCC3900.074.35326145
GAGCAAA52950.066.727291
CGAAAGC15450.066.6807253
GAGCGAA10150.066.474851
AGCGAAA16600.061.624221
GCAAAAG56650.061.4726223
GCGAAAG17600.058.945952
AGCAAAA62300.056.012842
CAAAAGC63600.056.0099034
TACGACG1402.7102942E-1046.639613
TTACGAC1402.7102942E-1046.6385462
CGACGGG1402.7102942E-1046.637495
AAAGCAG92050.045.2190176
ACGACGG1453.8198777E-1045.031354
GACGAAA10000.044.94655145
AAGCAGG99350.042.3324477
ATATCGT10800.042.3211251
AAAAGCA85600.042.290955
TATCGTG11000.041.550712
ATTACGA1601.0040822E-940.809661
Produced by FastQC (version 0.11.5)