FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n02_CM551457_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n02_CM551457_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28329
Sequences flagged as poor quality0
Sequence length151
%GC51

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGCAGGGAGAGCGCTTGCATGGCATGCAAGAGGTCAGCGGTTCGATCC1090.3847647287232165No Hit
CCCCTGTACTCACCGTGAAAGGGTGATGTCCTAGGCCTCTAGACGAAGGG990.3494652123265911No Hit
AGCAGGGAGAGCGCTTGCATGGCATGCAAGAGGTCAGCGGTTCGATCCCG990.3494652123265911No Hit
GGTCTGCACGCGCTCACGCGCAATCTCGCGATTGAACTCGCCGATGCGGG970.34240530904726607No Hit
CTTCTACGAAACGCCGCTGAATCTGGCGTCCCCTAGGGGATTCGAACCCC960.3388753574076035No Hit
TATTACTACCGCTGCTTCTCAAATCAATCCAGCTTGTTCTGTTGGTGGAG860.3035758410109782No Hit
TCTCAAATCAATCCAGCTTGTTCTGTTGGTGGAGGTAAGCGGGATCGAAC850.3000458893713156No Hit
TCGTAGAAGTGTCGCGAGGCTTGCAGGAACGTAACCGACGTCCTGCAGGT840.2965159377316531No Hit
GTCTAGAGGCCTAGGACATCACCCTTTCACGGTGAGTACAGGGGTTCGAA840.2965159377316531No Hit
TTTCAGATCCGTCTGACTTTCGCTATTACTTCCGGTCAACAGCGAAGGCC800.28239613117300294No Hit
CCCCTAGGGGATTCGAACCCCTGTACTCACCGTGAAAGGGTGATGTCCTA790.27886617953334036No Hit
CTTCTACGAAACGCCGCTAAATCTGGCGTCCCCTAGGGGATTCGAACCCC770.2718062762540153No Hit
GTCTTCGGGCGTACCGACGCGTCCGAGCGGATGAAACGAATCGAGCGCTT730.2576864696953652No Hit
AAGCAGGAGAGGGGGAGCAGGAGAAGGAGAAGGAGAAGGGGAAGGAGAAG700.2470966147763776No Hit
AGCGAAAGCAGGGAGAGCGCTTGCATGGCATGCAAGAGGTCAGCGGTTCG690.24356666313671504No Hit
AAGCAGGTCTGCACGCGCTCACGCGCAATCTCGCGATTGAACTCGCCGAT690.24356666313671504No Hit
GTAGTAATAAATGCTTCACAAAATGATTCAAAGCAGTTAGAATTTCGGCC680.2400367114970525No Hit
CCCTAGGGGATTCGAACCCCTGTACTCACCGTGAAAGGGTGATGTCCTAG620.21885700165907726No Hit
CTTTAATACAAAAGCCCGCTCAAAAACGTCTTGAACGGGCTTCGTTCTGG610.21532705001941474No Hit
GATTTGAGAAGCAGCGGTAGTAATAAATGCTTCACAAAATGATTCAAAGC580.2047371951004271No Hit
GCCTAGGACATCACCCTTTCACGGTGAGTACAGGGGTTCGAATCCCCTAG550.1941473401814395No Hit
AGCGAAAGCAGGTCTGCACGCGCTCACGCGCAATCTCGCGATTGAACTCG550.1941473401814395No Hit
GTCCTAGGCCTCTAGACGAAGGGGACATGATCTTTTCAGATCCGTCTGAC530.18708743690211443No Hit
CTCAAAAACGTCTTGAACGGGCTTCGTTCTGGCGGAGCGGACGGGACTCG520.1835574852624519No Hit
CTGTACGATCGCCGGCGAGACAGCGTTCACCCGGATACCCGCATCGGCGA520.1835574852624519No Hit
CTCCCAGGGAGGGAGATTAGCATGATGGAGAGACATTGAGAAAATGAAAT520.1835574852624519No Hit
ATTCTAACCGACTGAACTACCGCTCCAACTTTCTGCACCGTTACCTGCAG520.1835574852624519No Hit
CTTAGATACCGCCTTTCCCATCGAGATCAAACACTCTCCTTCTCTCATCC510.18002753362278937No Hit
CAGGAAGACAATCGTGTTCGCAACGTCTTCGGGCGTACCGACGCGTCCGA500.17649758198312684No Hit
GCTTACATCTTTAATACAAAAGCCCGCTCAAAAACGTCTTGAACGGGCTT500.17649758198312684No Hit
TTTATTACTACCGCTGCTTCTCAAATCAATCCAGCTTGTTCTGTTGGTGG500.17649758198312684No Hit
CTCACGCGCAATCTCGCGATTGAACTCGCCGATGCGGGTATCCGGGTGAA470.16590772706413923No Hit
ATCTAAGAAGGGCATCAAGTGATGTTCTAGTGAAGCGTTTAGGAGGCCGT470.16590772706413923No Hit
GTTCCTGCCTGCGTTGCGACACTTCTACGAAACGCCGCTGAATCTGGCGT470.16590772706413923No Hit
ATTCAAAGCAGTTAGAATTTCGGCCTTCGCTGTTGACCGGAAGTAATAGC460.16237777542447668No Hit
GTATTAAAGATGTAAGCAGTACGTTGTCCCCTTCGTCTAGAGGCCTAGGA460.16237777542447668No Hit
GAAGACAATCGTGTTCGCAACGTCTTCGGGCGTACCGACGCGTCCGAGCG460.16237777542447668No Hit
AGCGAAAGCAGGAAGACAATCGTGTTCGCAACGTCTTCGGGCGTACCGAC450.15884782378481416No Hit
GCATGCAAGAGGTCAGCGGTTCGATCCCGCTTACCTCCACCAACAGAACA440.1553178721451516No Hit
ATACAAAAGCCCGCTCAAAAACGTCTTGAACGGGCTTCGTTCTGGCGGAG430.1517879205054891No Hit
AGCGAAAGCAGGGGCCCTCCACTGAGTGAGTGCATTAAACAAAGCAGACA430.1517879205054891No Hit
GGATATCGTCACGTTCCTGCCTGCGTTGCGACACTTCTACGAAACGCCGC430.1517879205054891No Hit
CCTGTACTCACCGTGAAAGGGTGATGTCCTAGGCCTCTAGACGAAGGGGA420.14825796886582654No Hit
CCCCTAGGGGACGCCAGATTTAGCGGCGTTTCGTAGAAGTGTCGCGAGGC420.14825796886582654No Hit
CTATTACTTCCGGTCAACAGCGAAGGCCGAAATTCTAACTGCTTTGAATC420.14825796886582654No Hit
ACAAAAGCCCGCTCAAAAACGTCTTGAACGGGCTTCGTTCTGGCGGAGCG410.14472801722616402No Hit
GGACATGGAGAGCCAGAGAGATATGAGAGGAAGAGGATGGATGGATGGAT410.14472801722616402No Hit
GTTTAGGAGGCCGTGGGAGAGAGAACACTGGAGTGTTTTACTCTCTTCCT410.14472801722616402No Hit
ATCGTACAGACGTCGATTTATGAAGGCTTCATGGCGAAGGAAGACATTGC410.14472801722616402No Hit
GTAATAAATGCTTCACAAAATGATTCAAAGCAGTTAGAATTTCGGCCTTC410.14472801722616402No Hit
TCTTAGATACCGCCTTTCCCATCGAGATCAAACACTCTCCTTCTCTCATC400.14119806558650147No Hit
GTTACCTGCAGGACGTCGGTTACGTTCCTGCAAGCCTCGCGACACTTCTA400.14119806558650147No Hit
GTCTTCCTTCGCCATGAAGCCTTCATAAATCGACGTCTGTACGATCGCCG400.14119806558650147No Hit
TTTCTGCACCGTTACCTGCAGGACGTCGGTTACGTTCCTGCAAGCCTCGC400.14119806558650147No Hit
GTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTCCGCCAGAACGAA400.14119806558650147No Hit
GTCCTAGGCCTCTAGACGAAGGGGACAACGTACTGCTTACATCTTTAATA390.13766811394683892No Hit
GAGTACAGGGGTTCGAATCCCCTAGGGGACGCCAGATTTAGCGGCGTTTC390.13766811394683892No Hit
TCCCGGAATCGGGCACGACGGTGATCGACTGCTGCGGCTGCCCGCGCGTC390.13766811394683892No Hit
CCCTAGGGGACGCCAGATTTAGCGGCGTTTCGTAGAAGTGTCGCGAGGCT390.13766811394683892No Hit
AGCGAAAGCAGGCAGGATATCGTCACGTTCCTGCCTGCGTTGCGACACTT380.1341381623071764No Hit
GTTAGAATTTCGGCCTTCGCTGTTGACCGGAAGTAATAGCGAAAGTCAGA380.1341381623071764No Hit
CTTGCATGGCATGCAAGAGGTCAGCGGTTCGATCCCGCTTACCTCCACCA380.1341381623071764No Hit
GATCTGAAAAGATCATGTCCCCTTCGTCTAGAGGCCTAGGACATCACCCT380.1341381623071764No Hit
TTCTTAGATACCGCCTTTCCCATCGAGATCAAACACTCTCCTTCTCTCAT370.13060821066751385No Hit
GTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCC370.13060821066751385No Hit
AGCGAAAGCAGGCGGCCGCGAGCGTCGAGCAACTGACGGCGGCCGTGCGC360.1270782590278513No Hit
GCAGGTAACGGTGCAGAAAGTTGGAGCGGTAGTTCAGTCGGTTAGAATAC360.1270782590278513No Hit
GTATTCTAACCGACTGAACTACCGCTCCAACTTTCTGCACCGTTACCTGC350.1235483073881888No Hit
TTGCAGGTGCGATGAAAGCGCTCGATTCGTTTCATCCGCTCGGACGCGTC350.1235483073881888No Hit
ATGTTGAAGACTGAAAAAGAAAGGATGAGAGGATGAGGATGAGAGGATGA350.1235483073881888No Hit
ATTTATTACTACCGCTGCTTCTCAAATCAATCCAGCTTGTTCTGTTGGTG340.12001835574852625No Hit
AGACAATCGTGTTCGCAACGTCTTCGGGCGTACCGACGCGTCCGAGCGGA340.12001835574852625No Hit
GTACAGGGGTTCGAATCCCCTAGGGGACGCCAGATTTAGCGGCGTTTCGT340.12001835574852625No Hit
CCCTGGGAGATGCTATCAAATACTCTCCTCCTTCTCCTTCCCCTTCTCCT340.12001835574852625No Hit
AGCGAAAGCAGGACATGGAGAGCCAGAGAGATATGAGAGGAAGAGGATGG330.1164884041088637No Hit
GAGTAAAACACTCCAGTGTTCTCTCTCCCACGGCCTCCTAAACGCTTCAC330.1164884041088637No Hit
GCAGGAAGACAATCGTGTTCGCAACGTCTTCGGGCGTACCGACGCGTCCG330.1164884041088637No Hit
GGCTTCGTTCTGGCGGAGCGGACGGGACTCGAACCCGCGACCCCCGGCGT320.11295845246920118No Hit
AATAAATGCTTCACAAAATGATTCAAAGCAGTTAGAATTTCGGCCTTCGC320.11295845246920118No Hit
GATGAAACGAATCGAGCGCTTTCATCGCACCTGCAATGTCTTCCTTCGCC320.11295845246920118No Hit
GATCTTTTCAGATCCGTCTGACTTTCGCTATTACTTCCGGTCAACAGCGA320.11295845246920118No Hit
GCTTTGAATCATTTTGTGAAGCATTTATTACTACCGCTGCTTCTCAAATC320.11295845246920118No Hit
GGCCTAGGACATCACCCTTTCACGGTGAGTACAGGGGTTCGAATCCCCTA320.11295845246920118No Hit
AGCGAAAGCAGGGCGAGAGGAGAGGAGAGGAGAGGAGAGGAGAGGAGAGG320.11295845246920118No Hit
GTACAGGGGTTCGAATCCCCTAGGGGACGCCAGATTCAGCGGCGTTTCGT310.10942850082953863No Hit
GTAATAGCGAAAGTCAGACGGATCTGAAAAGATCATGTCCCCTTCGTCTA310.10942850082953863No Hit
GAAGTAATAGCGAAAGTCAGACGGATCTGAAAAGATCATGTCCCCTTCGT310.10942850082953863No Hit
TCCTAGGCCTCTAGACGAAGGGGACAACGTACTGCTTACATCTTTAATAC300.1058985491898761No Hit
AGTAATAAATGCTTCACAAAATGATTCAAAGCAGTTAGAATTTCGGCCTT300.1058985491898761No Hit
CAGGGAGAGCGCTTGCATGGCATGCAAGAGGTCAGCGGTTCGATCCCGCT300.1058985491898761No Hit
TCATAAATCGACGTCTGTACGATCGCCGGCGAGACAGCGTTCACCCGGAT300.1058985491898761No Hit
CGTCTAGAGGCCTAGGACATCACCCTTTCACGGTGAGTACAGGGGTTCGA300.1058985491898761No Hit
AGCGAAAGCAGGGTCAAGGTTCAGAGGTCAAAGGTCACAGGTCAAGGGTC300.1058985491898761No Hit
GGCAGGATATCGTCACGTTCCTGCCTGCGTTGCGACACTTCTACGAAACG300.1058985491898761No Hit
GGAGAGGGGGAGCAGGAGAAGGAGAAGGAGAAGGGGAAGGAGAAGGAGGA290.10236859755021355No Hit
AAATGATTCAAAGCAGTTAGAATTTCGGCCTTCGCTGTTGACCGGAAGTA290.10236859755021355No Hit
TCGCTGTTGACCGGAAGTAATAGCGAAAGTCAGACGGATCTGAAAAGATC290.10236859755021355No Hit
CTTTCATCGCACCTGCAATGTCTTCCTTCGCCATGAAGCCTTCATAAATC290.10236859755021355No Hit
GCATTTATTACTACCGCTGCTTCTCAAATCAATCCAGCTTGTTCTGTTGG290.10236859755021355No Hit
AATCAATCCAGCTTGTTCTGTTGGTGGAGGTAAGCGGGATCGAACCGCTG290.10236859755021355No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCATAG100.0070284954145.180533
GTAAACA100.0070284954145.180534
GGTCTGC458.0035534E-11112.918191
TGCACGC553.929017E-1092.387615
GTCTGCA553.929017E-1092.387612
CTGCACG553.929017E-1092.387614
ACGCGCT553.929017E-1092.387618
AAGGGTC259.2461135E-487.1083155
GAGCGAA502.2321092E-672.590261
GGGTCAA300.001906825572.590267
GGTCAAG300.001906825572.590261
CGGTGGG300.001920284972.46201145
GCACGCG754.5856723E-967.7509166
CGCGCTC754.5856723E-967.7509169
CGAAAGC1009.094947E-1265.331243
GCAGGTC350.003513381162.2202268
TCTCGGG350.003538146462.110294145
TCTGCAC1006.693881E-1058.0722123
GAAAGCA1205.4569682E-1154.44274
GTCAAGG450.00949640248.393512
Produced by FastQC (version 0.11.5)