FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n02_CM551087_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n02_CM551087_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1201481
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACCATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA18850.15688970528872284No Hit
ATATCGTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCC16030.13341867245507835No Hit
GAGCAAAAGCAGGTGGGGAGGTAAGAGAGGAGTAGGGGAGGGCCAGAGAC14870.12376392136038772No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTACGG755.638867E-1177.32581145
GAGCAAA28100.074.6064761
GAGCGAA5650.074.467121
GCAAAAG32450.063.487563
AGCAAAA33000.062.4294362
AGCGAAA8850.059.8362851
CAAAAGC35200.059.5580184
CGAAAGC9250.058.0329973
CGACGGG953.1270247E-853.4492235
ATATCGT8200.051.309661
GCGAAAG10500.051.124312
ATTACGA1004.683352E-850.7788731
TACGACG1004.683352E-850.7788733
TATCGTG8300.050.6914752
AAAGCAG50550.049.2054676
CATACCG901.3212721E-648.346749
TTACGAC1109.91713E-846.1626172
ACGACGG1151.4066063E-744.1555444
AAGCAGG56550.043.856497
ATCGTGT9600.043.8270033
Produced by FastQC (version 0.11.5)