FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n02_CM550443_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n02_CM550443_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2323568
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA25850.0113.669641
CGAAAGC30250.096.656193
GCGAAAG35750.082.5962
GAGCGAA6700.080.132171
TACGCCC1405.456968E-1251.82075
TGACGCC6300.050.626602145
ATTACGC1602.0008883E-1145.3450623
GAAAGCA70700.042.895024
GCAAAAG36850.042.330263
GACGAAA13750.039.011772145
GAGCAAA40500.038.8735961
TCGACAG750.001484989438.660313145
CAAAAGC41600.037.148074
AGCAAAA43650.035.4026682
GATTACG2154.838512E-1033.744442
AAAGCAG110400.033.3830455
CAGTACG1801.2899E-732.2328839
TAGAAAC19600.032.2035562
CTATTCG7300.031.7913369
TTACGCC2351.2551027E-930.8732364
Produced by FastQC (version 0.11.5)