FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n02_CM550292_2_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n02_CM550292_2_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1632641
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA18340.11233332986247437No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG17430.10675953868609205No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG16990.10406451877663246No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT16620.1017982520345869No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA6800.070.4253541
TGACGCC3350.067.078766145
CGAAAGC10200.065.449813
GAGCAAA39800.060.89161
GCGAAAG11650.057.3001752
GCAAAAG41400.057.1396983
AGCAAAA42100.056.5308882
AGCGAAA12000.056.233581
CAAAAGC42900.056.156684
CGACGAA10050.053.374504145
TCTCGGG7200.046.312145
AAGCAGG64950.043.9944737
CATACCG1002.7284423E-643.5156639
ATATCGT6900.042.0634541
AAAAGCA59800.041.741175
CGTGTAG7000.041.4638185
TATCGTG7000.041.4625472
GTCGCCC4900.041.421913145
GACGCTC1252.723791E-740.593475145
AAAGCAG71600.040.3147936
Produced by FastQC (version 0.11.5)