FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n02_CM550292_1_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n02_CM550292_1_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences423821
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCGTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCC8620.20338775096090095No Hit
ACACCATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA8140.19206221494451667No Hit
GAGCAAAAGCAGGTGGGGAGGTAAGAGAGGAGTAGGGGAGGGCCAGAGAC5860.13826591886669135No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA5250.12387305017920303No Hit
GGACCAATACATGGGTACCACACTGGGTGCTGAGACTTGGCCTTCTTCAC5120.12080571750809894No Hit
ATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCAAGGCA4310.10169387548045047No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA1950.078.0984041
GAGCAAA10850.074.190911
AGCAAAA11200.071.2249452
GCAAAAG11200.067.987453
CAAAAGC12150.062.6715554
CCGATAC350.003562867962.152629
GGACCGA350.003562867962.152626
GGGACCG502.0061866E-458.0159575
CGAAAGC3100.056.1444743
GTCGCCC1301.8189894E-1255.76483145
TGACGCC400.006050379554.37071145
AGCGAAA3250.053.5531921
GCGAAAG3400.053.3234862
TACCGGG553.2125151E-452.741781
AAAGCAG18200.050.5986066
AAAAGCA16100.047.7460565
ATATCGT3000.043.511971
AAGCAGG21250.043.3362167
AATTAAC855.3276082E-542.658792
CCGACGC855.33875E-542.643692145
Produced by FastQC (version 0.11.5)